Identification of novel biomarker candidates by proteomic analysis of cerebrospinal fluid from patients with moyamoya disease using SELDI-TOF-MS (original) (raw)

The aim of this study was the identification of novel biomarker candidates for the diagnosis of cholangiocellular carcinoma (CCC) and its immunohistochemical differentiation from benign liver and bile duct cells. CCC is a primary cancer that arises from the epithelial cells of bile ducts and is characterized by high mortality rates due to its late clinical presentation and limited treatment options. Tumorous tissue and adjacent non-tumorous liver tissue from eight CCC patients were analyzed by means of twodimensional differential in-gel electrophoresis and massspectrometry-based label-free proteomics. After data analysis and statistical evaluation of the proteins found to be differentially regulated between the two experimental groups (fold change > 1.5; p value < 0.05), 14 candidate proteins were chosen for determination of the cell-typespecific expression profile via immunohistochemistry in a cohort of 14 patients. This confirmed the significant upregulation of serpin H1, 14-3-3 protein sigma, and stressinduced phosphoprotein 1 in tumorous cholangiocytes relative to normal hepatocytes and non-tumorous cholan-From the ‡Medizinisches Proteom-Center, Ruhr-Universitä t Bochum, 44801 Bochum, Germany; ¶Institut fü r Pathologie, Universitä tsklinikum Essen, Universitä t Duisburg-Essen, 45141 Essen, Germany; ʈKlinik fü r Gastroenterologie und Hepatologie, Universitä tsklinikum Essen, 45141 Essen, Universitä t Duisburg-Essen,