Characterization of the xylanolytic enzyme system of the extreme thermophilic anaerobic bacteria Thermotoga maritima, T. neapolitana, and T. thermarum (original) (raw)

1997, Comparative Biochemistry and Physiology - A Physiology

The extreme thermophilic anaerobic bacteria Thermotoga maritime, T. neapolitana, and T. thermarum when grown on xylan produce extremely thermoactive xylanases, /%xylosidases and u-arabinofuranosidases. Most of these enzymes are active over a broad temperature and pH range, namely between 40 and 110°C and pH 4 to 9. The xylanases are active towards soluble, as well as insoluble xylan. Unlike the enzyme system of T. mmitima and T. themrum, the xylanase activity of T. neapolitana is activated (up to 211%) in the presence of Ca*+, Mg", Co*+, and Mn*+. The xylanolytic enzyme system of T. themurrum is inactive towards cellulose. Xylan hydrolysis experiments indicate the presence of endoxylanases in the enzyme preparation of all strains investigated, with the main products being xylobiose, xylotriose, and larger xylooligosaccharides. COMP RIOCHEM PHYSIOL 118A:3:453-461, 1997. 0 1997 Elsevier Science Inc.

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