Phylogeny of the true water bugs (Nepomorpha: Hemiptera-Heteroptera) based on 16S and 28S rDNA and morphology (original) (raw)

2004, Systematic Entomology

Morphological characters and molecular sequence data were for the first time analysed separately and combined for the true water bugs (Hemiptera-Heteroptera, infraorder Nepomorpha). Data from forty species representing all families were included, together with two outgroup species representing the infraorders Gerromorpha and Leptopodomorpha. The morphological data matrix consisted of sixty-five characters obtained from literature sources. Molecular data included approximately 960 bp from the mitochondrial gene 16S and the nuclear gene 28S for all forty-two terminal taxa. The morphological dataset was analysed using maximum parsimony and the combined morphological and molecular (16S รพ 28S rDNA) dataset was analysed using direct optimization. A sensitivity analysis of sixteen different sets of parameters (various combinations of insertion-deletion cost and transversion costs) was undertaken.

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