Isolation of plasmids present in thermophilic strains from hot springs in Jordan (original) (raw)
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Characterization of Thermophilic Bacteria Isolated from two Hot Springs in Jazan, Saudi Arabia
2017
The capability of thermophilic microorganisms to flourish at high temperatures makes their enzyme systems ideal for various biotechnological applications. Based on the proteolytic and amylolytic activities, two thermophilic bacteria were isolated from hot springs in Jazan, Saudi Arabia. The antibiotic and heavy metals susceptibility patterns of the bacterial isolates were performed. According to the metabolic fingerprint, the bacterial isolates were identified as Brevibacterium linens and Bacillus subtilis. Moreover, the impact of temperature, substrate concentration, and some metal ions on the production of proteases and amylases from the bacterial strains was investigated. The maximum protease production was achieved at 50°C while the greatest amylase production was recorded at 30°C for both strains at a constant pH of 7.5. The highest enzyme production was recorded at 5% skimmed milk for protease of B. linens and 10% for B. subtilis while 0.6% starch was the optimum substrate concentration of amylase production of the two strains. Furthermore, Ca 2+ showed a simulative influence on protease production from the two strains whereas Mg 2+ and Mn 2+ demonstrated minor effect. On the other hand, Ca 2+ , Mg 2+ and Mn 2+ demonstrated a positive effect on the amylase production from both strains.
Characterization and 16S rDNA Identification of Thermo-tolerant Bacteria Isolated from Hot Springs
2007
High water temperature exerts selection pressure on microbial species leading to specific flora that survives and tolerates heat stress. A total of 229 bacterial cultures were isolated from ten different hot springs at Siwa, Matrouh, Egypt. The number and percentage of heat tolerant bacteria were assessed. Only 13 (5.6%) of bacterial isolates were able to tolerate and survive 65°C. These bacterial isolates were genetically diverse according to RAPD and Box-PCR analyses using different primers. RAPD, Box-PCR and 16S rRNA sequence analysis confirmed the abundance of bacterial genotypes and that they were closely related to Bacillus licheniformis and Bacillus pumilus, based on 100% similarity in their 16S rDNA gene sequences. Bacillus licheniformis responded to one hour of thermal stress at elevated temperature from 30°C to 65°C by synthesizing different heat shock proteins (HSPs) with molecular weights ranging between .30-120 kDa.
Plasmid, 1999
We have screened Thermotoga strains, isolated from hydrothermal vents near the Kuril Islands, for the presence of plasmid DNA. The miniplasmid pMC24 was isolated from the extreme thermophilic eubacteria Thermotoga maritima and sequenced, showing it to be a plasmid of 846 bp. It was found, from a search of the databases, to be closely related to the previously described Thermotoga miniplasmid pRQ7, isolated from a strain found on the Azore Islands, and was distinguished by only two point mutations. These changes resulted in two consecutive frameshifts altering a region encoding 9 amino acids in the Rep-coding region. We have also shown that pMC24, as with pRQ7, is negatively supercoiled. It seems that negatively supercoiled miniplasmids related to pRQ7 are spread worldwide and strongly maintained among Thermotoga strains.
Physical characterization of a plasmid (pTT1) isolated from Thermus thermophilus
Plasmid, 1981
A small circular supercoiled DNA molecule species with a molecular weight of about 5.4 x IO6 has been isolated from the extreme thermophile Thermus thermophilus HB8. This plasmid (pTT1) has a G plus C content of 68%, similar to that of the host chromosome. The superhelix density is the same as that of bacteriophage PM2 DNA. A physical map of the plasmid has been obtained using restriction endonucleases.
Thermophilic bacteria that tolerate a wide temperature and pH range colonize the Soldhar (95 °C) and Ringigad (80 °C) hot springs of Uttarakhand, India Your article is protected by copyright and all rights are held exclusively by Springer-Verlag Berlin Heidelberg and the University of Milan. This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to selfarchive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".
Thermophilic bacteria from the hot springs of Gilgit (Pakistan)
Journal of Animal and Plant Sciences, 2012
The importance of thermostable biomolecules in the field of biotechnology has spurred research into organisms capable of growth at high temperatures. Three thermophilic bacterial strains GCTP-1, GCMB-1 and GCDP-1 were isolated from the hot springs of Tatta Pani, Murtazabad & Darkut Pass respectively in the surroundings of Gilgit. All isolates have entire and slimy colonies while the cells were small rods, gram-negative non-motile and semi aerobic. Strains GCTP-1 showed positive results of ortho nitrophenyl-β-D-galactopyranosidase (ONPG) and gelatin hydrolysis (GEL) tests other isolates gave negative results in all tests such as ortho nitrophenyl-βD-galactopyranosidase, arginine dihydrolases, lysine decarboxlase, ornithine decarboxylases, citrate utilization, H2S production, urease, tryptophan deaminases, indole production, acetoin production, gelatin hydrolysis, Fermentation/oxidation (glucose, mannitol, inositol, sorbitol, rhamnose, sucrose, melibiose, amygdalin, arabinose) and cyt...
Annals of Microbiology, 2014
Thermophilic bacteria that tolerate a wide temperature and pH range colonize the Soldhar (95 °C) and Ringigad (80 °C) hot springs of Uttarakhand, India Your article is protected by copyright and all rights are held exclusively by Springer-Verlag Berlin Heidelberg and the University of Milan. This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to selfarchive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".
Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils
Brazilian Journal of Microbiology, 2015
The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5%) represented by B. licheniformis (119), B. aerius (44), B. sonorensis (33), B. subtilis (subsp. spizizenii (2) and subsp. inaquosurum (6)), B. amyloliquefaciens (subsp. amyloliquefaciens (4) and subsp. plantarum (4)), B. tequilensis (3), B. pumilus (3) and Bacillus sp. (19). Only six isolates (2.5%) belonged to the genus Aeribacillus represented by A. pallidus (4) and Aeribacillus sp. (2). In this study, B. aerius and B. tequilensis are described for the first time as thermophilic bacteria. Moreover, 71.25%, 50.41% and 5.41% of total strains exhibited high amylolytic, proteolytic or cellulolytic activity respectively.
Brazilian Journal of Microbiology, 2020
This study aims to determine the diversity of culturable thermophilic bacteria isolated from eight terrestrial hot springs in Northeastern of Algeria using the conventional methods, SDS-PAGE fingerprinting of whole-cell proteins and 16S rRNA gene sequencing. In addition, their hydrolytic enzyme activities were also investigated. A total of 293 strains were isolated from the hot springs' water and sediment using different culture media. Overall, five distinct bacterial groups were characterized by whole-cell protein pattern analysis. Based on the 16S rRNA gene sequencing of 100 selected strains, the isolates were assigned to the following three major phyla: Firmicutes (93%), Deinococcus-Thermus (5%), and Actinobacteria (2%), which included 27 distinct species belonging to 12 different phylotypes, Aeribacillus, Aneurinibacillus, Anoxybacillus, Bacillus, Brevibacillus, Geobacillus, Laceyella, Meiothermus, Saccharomonospora, Thermoactinomyces, Thermobifida, and Thermus. The screening for nine extracellular enzymes showed that 65.87% of the isolates presented at least five types of enzyme activities, and 6.48% of strains combined all tested enzymes (amylase, cellulase, pectinase, esculinase, protease, gelatinase, lipase, lecithinase, and nuclease). It was found that Bacillus, Anoxybacillus, Aeribacillus, and Aneurinibacillus were the genera showing the highest activities. Likewise, the study showed an abundant and diverse thermophilic community with novel taxa presenting a promising source of thermozymes with important biotechnological applications. This study showed that a combined identification method using SDS-PAGE profiles of whole-cell proteins and subsequent 16S rRNA gene sequence analysis could successfully differentiate thermophilic bacteria from Algerian hot springs.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Microbiology journal is one of the series issued twice by the Egyptian Academic Journal of Biological Sciences, and is devoted to publication of original papers related to the research across the whole spectrum of the subject. These including bacteriology, virology, mycology and parasitology. In addition, the journal promotes research on the impact of living organisms on their environment with emphasis on subjects such a resource, depletion, pollution, biodiversity, ecosystem…..etc www.eajbs.eg.net Provided for non-commercial research and education use. Not for reproduction, distribution or commercial use.