Characterization of novel microsatellite loci in the great leaf-nosed bat, Hipposideros armiger and cross-amplification in other related species (original) (raw)
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Conservation Genetics, 2008
Dendrocincla turdina has a poor ability to survive in fragmented areas and inhabits the depleted Brazilian Atlantic Forest. For the assessment of genetic diversity in this species, nineteen microsatellite loci were isolated and characterized, 11 of which were polymorphic for the population studied. Observed and expected heterozigosity ranged from 0.07 to 0.80 and 0.08 to 0.91, respectively. Cross-amplification was successfully obtained with two other species from the family Dendrocolaptidae: Xiphorhynchus fuscus and Sittasomus griseicapillus. The newly developed primers reported here constitute a useful tool for genetic population analyses on D. turdina and, potentially, other related species.
Conservation Genetics Resources, 2014
We isolated and characterized 16 polymorphic microsatellite loci for Lytocarpia brevirostris (Aglaopheniidae), a hydrozoan common in the tropical Indo-Pacific region. Four to 34 alleles per locus were detected at the Indo-Pacific scale. At the population level, observed (H o) and expected (H e) heterozygosities ranged across 0.100-0.625 and 0.097-0.597, respectively. Three markers showed significant deviation from Hardy-Weinberg equilibrium, all of them presenting null alleles. Linkage disequilibrium was detected in three pairs of loci among 120. These primers provide powerful tools for studying population genetic diversity and the implication of life cycle strategies on population differentiation in tropical hydrozoans. This will be valuable for the conservation of coral reefs biodiversity and the design of marine protected areas.
Heredity, 2012
It has been postulated that obligate asexual lineages may persist in the long term if they escape from negative interactions with either sexual lineages or biological enemies; and thus, parthenogenetic populations will be more likely to occur in places that are difficult for sexuals to colonize, or those in which biological interactions are rare, such as islands or island-like habitats. Ischnura hastata is the only known example of natural parthenogenesis within the insect order Odonata, and it represents also a typical example of geographic parthenogenesis, as sexual populations are widely distributed in North America, whereas parthenogenetic populations of this species have only been found at the Azores archipelago. In order to gain insight in the origin and distribution of parthenogenetic I. hastata lineages, we have used microsatellites, mitochondrial and nuclear DNA sequence data, to examine the population genetic structure of this species over a wide geographic area. Our resul...
Conservation Genetics Resources, 2012
In this study we used Roche's 454 sequencing to develop genetic markers for Coenagrion scitulum, a Mediterranean damselfly currently expanding its range northward. With a modest amount of sequencing we detected 6,318 potential microsatellite markers and 832 putative single nucleotide polymorphisms (SNPs). From the potential microsatellite markers we developed thirteen novel microsatellite markers. Among other applications, these markers can be used to unravel the micro-evolutionary consequences of range expansion in this species.
Molecular Ecology …, 2002
The Bang's leaf-nose bat, Hipposideros turpis, is an endangered cave-dwelling species inhabiting the southwesternmost islands of Japan. We isolated six dinucleotide microsatellite markers from the partial genomic library of the bat, and examined their allelic variation using a sample (N = 33) from the largest colony in Japan. All the loci showed a moderate allelic variation ranging from two to eight alleles, with the observed heterozygosities from 0.33 to 0.73, and conformed to Hardy–Weinberg expectations. The present microsatellite markers will be useful in assessing population genetic structure and gene flow among populations of this species.
Journal of Genetics, 2018
Pteronotus psilotis, a mormoopid bat, is an insectivorous, gregarious and strict cave-dwelling species that is found areas between the sea level and an elevation of about 1000 masl. This species is present in diverse habitats ranging from rain forest to dry deciduous forest. Nine microsatellite loci were developed for Wagner's mustached bat, Pteronotus psilotis using the next-generation sequencing approach, and their utility for population genetics studies was assessed. All loci were polymorphic (7-15 alleles) and characterized in 30 individuals from three P. psilotis populations, with the levels of observed and expected heterozygosity ranging from 0.280 to 0.867 and 0.584 to 0.842, respectively. One locus showed significant departures from Hardy-Weinberg expectations after Bonferroni correction. Cross-amplification in 11 other bat species was tested, for which eight microsatellites were successfully amplified, and of these seven were polymorphic. The development of these new microsatellite loci will contribute to investigations of genetic population structure, genetic diversity and gene flow in P. psilotis populations, as well as in other closely related bat species.
2008
Pistacia chinensis Bunge (Anacardiaceae) is a native, dioecious, perennial arbor, and an important bioenergy plant. Twelve microsatellite markers were developed for P. chinensis to evaluate genetic diversity and population genetic structure when this species was subject to landscape fragmentation. Twelve polymorphic microsatellite loci were developed in P. chinensis using a microsatellite-enriched genomic library based on magnetic beads. These loci were characterized in 24 individuals from three populations located on Thousand Island Lake, Zhejiang Province, China. The number of alleles per locus varied from three to sixteen. The observed and expected heterozygosity ranged from 0.1250 to 0.8750 and 0.2333 to 0.8917, respectively. These microsatellite loci will be applied in further studies on the population genetic diversity and genetic structure of P. chinensis. This study will improve understanding of the effects of landscape fragmentation, and help conserve and manage the species.
Molecular Ecology Notes, 2007
We isolated and characterized 10 polymorphic microsatellite loci from the azure damselfly Coenagrion puella (Zygoptera; Coenagrionidae) as part of a study assessing reproductive success and genetic structure in an isolated population of this species. Levels of genetic diversity were assessed in 50 individuals collected from Queen Elizabeth Country Park, Hampshire, UK. The number of alleles per microsatellite loci ranged from three to 22 and the observed and expected heterozygosities varied between 0.26 and 0.84 and between 0.23 and 0.91, respectively. Two loci showed significant ( P < 0.05) heterozygote deficits, likely because of null (non-amplifying) alleles; one pair of loci was in linkage disequilibrium.
2006
One of the most widely distributed dragonfly species in Africa is the red-veined-dropwing Trithemis arteriosa. It is an indicator for permanent water bodies, which are freshwater ecosystems of high environmental value especially in arid regions. For studies to determine population structures, assess species viability and monitor environmental changes, a panel of 10 polymorphic microsatellite loci was developed. The number of alleles per locus ranged from four to 12, with an observed heterozygosity ranging from 0.149 to 0.843.
Genetics and Molecular Research, 2011
Twelve polymorphic microsatellite loci were isolated from an (AC) n-and (AG) n-enriched DNA library for the endemic Chinese frog Pelophylax hubeiensis (Ranidae). The number of alleles per locus ranged from two to eight, with a mean of 5.17. The observed and expected heterozygosities ranged from 0.226 to 0.839 and from 0.204 to 0.826, with means of 0.568 and 0.656, respectively. No significant linkage disequilibrium was detected among these loci. However, two significant deviations from HWE were discovered at loci Pehu-11 and Pehu-12 (P < 0.05). MICRO-CHECKER tests showed that null alleles could be present at locus Pehu-12. These polymorphic microsatellite loci can be employed for exploring mating mechanisms, population genetic structure and other relevant genetic investigations of P. hubeiensis.