Engineered Heparan Sulfate-Collagen IV Surfaces Improve Human Mesenchymal Stem Cells Differentiation to Functional Hepatocyte-Like Cells (original) (raw)

Journal of Biomaterials and Tissue Engineering, 2014

Abstract

ABSTRACT Liver failure causes many deaths annually while patients awaiting on transplantation list. Poor functional hepatocytes for transplantation are unable to overcome the difficulty. An increasing number of studies on mesenchymal stem cells differentiation toward hepatocyte-like cells made it a promising alternative strategy. To meet the demand for human hepatocyte sourcing that has been a bottle-neck for many research and clinical areas, this study aimed to introduce a new matrix to mimic the liver microenvironment and improve hepatic differentiation. The matrix was composed of heparan sulfate and collagen type IV. Heparan Sulfate-collagen matrix significantly increased the yield of human mesenchymal stem cell-derived hepatocytes. Elevated CYP3A4 enzyme activity, alpha-fetoprotein, albumin and c-MET protein levels in this hepatocyte-like cells demonstrated improved hepatic differentiation and functionality.

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