Conventional mechanical ventilation of healthy lungs induced pro-inflammatory cytokine gene transcription (original) (raw)

2002, Respiratory Physiology & Neurobiology

We investigated the potential inflammatory reaction induced by mechanical ventilation (MV) using 10 ml/kg tidal volume and no positive end-expiratory pressure (PEEP) in control (C, n= 8), spontaneously breathing (SB, n =12) and mechanically ventilated (MV, n= 12) rabbits with normal lungs. After 6 h (MV and SB groups) or immediately (C group), lungs were removed for measurement of wet-to-dry (W/D) weight ratio and for bronchoalveolar lavage (BAL). Pulmonary mechanics were also studied. MV animals developed a modest but significant (P B 0.01) impairment of arterial blood oxygenation and had higher W/D lung weight ratio than C ones. In MV group, BAL macrophage count was greater (PB0.05) than in SB one. MV induced an upregulation of MCP-1, TNF-a, and IL-1b gene transcription (mRNAs), without significant elevation of the corresponding protein cytokines in the BAL supernatant, except for MCP-1 (PB 0.05). These data suggest that MV, even using moderate tidal volume, elicits a pro-inflammatory stimulus to the lungs.

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