Effect of varicocelectomy on sperm creatine kinase, HspA2 chaperone protein (creatine kinase-M type), LDH, LDH-X, and lipid peroxidation product levels in infertile men with varicocele (original) (raw)
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Mean Changes in Sperm Count in Adult Males after Varicocelectomy
Pakistan Journal of Medical and Health Sciences, 2021
Background: Varicocele, a bilateral vascular condition in which hypoxia can induce ischemic damage to both testes due to hydrostatic pressures in the venous drainage system, is a prominent cause of male infertility with a complex pathogenesis. varicocele make up 35% of primary infertility and 81% of secondary fertility. Methodology: A quasi-experimental study was conducted from January 2018 to September 2018 at urology department of Nawaz Sharif Kidney Hospital and Saidu Group of Teaching Hospitals, Swat. Sample size was 261. The study included all males between the ages of 20 and 60 who had clinically evident varicocele. Result: Results of our study P-value for pre-operative and post-operative sperm count was 0.00001 so statistically significant. According to the age distribution, 93 patients were in the age group 20-30 (35.63%), 70 patients in age group 31-40 years (27.01%), 60 patients aged 41-50 (22.98%), 38 patients aged 51 to 60 (14.26) %) are shown in Table 2. All cases had v...
Human Andrology, 2013
Background Varicocele is the most common cause of male infertility. Varicocele affects spermatogenesis negatively by increasing the levels of reactive oxygen species. Varicocelectomy may improve spermatogenesis and semen parameters. Aim To detect the efficacy of microsurgical varicocelectomy in decreasing seminal oxidative stress and improving sperm parameters and sperm ultrastructure in infertile male patients. Methods Twenty-five infertile patients with third-degree varicocele were included in this study. Assessment of the levels of lipid peroxidation in seminal plasma, semen analysis and transmission electron microscopy examination were carried out before and 4 months after microsurgical varicocelectomy. Results A significant decrease in the malondialdehyde level was found after varicocelectomy (Po0.01). All sperm parameters except abnormal tail and ultrastructural sperm abnormalities improved significantly after varicocelectomy. Limitations The small sample and the lack of a control group are the main limitations of this study. Conclusion Although there is no conclusive evidence that varicocele repair improves spontaneous pregnancy rates, it was found that microsurgical varicocelectomy improved sperm parameters and sperm ultrastructure.
Objectives:-To evaluate the efficacy of varicocelectomy in patients with clinical versus subclinical varicocole as regards the seminal parameters.Patients and Methods:-A total of 162 patients with varicocele (72 subclinical and 90 clinical) were included in the study. Semen analysis was done for all patients before and after 3 to 6 months of varicocelectomy. Improvements of semenial parameters were statistically significant in patients with clinical varicocele (P< 0.05). Bilateral and right side varicocele were more common in patients with subclinical varicocele. Also there incidence secondary infertility higher patients presented with subclinical varicocele compared with patients presented with clinical varicocler (P< 0.05).As regard, the effects of varicocelectomy in patients with subclinical varicocele and the clinical differences between these patients and patients with clinical varicocle, we recommend avoiding varicocelectomy in this group of patients unless no identifiab...
Spermogram changes and pregnancy rate in Egyptian infertile males before and after varicocelectomy
Journal of …, 2012
Background: Varicoceles are abnormally dilated testicular veins in the scrotum, secondary to internal spermatic vein reflux. Varicocele is found in approximately 15% of the general population, 35% of men with primary infertility and in 7581% of men with secondary infertility. Although there have been numerous theories regarding the pathophysiology of varicocele, some remains unknown about its role in management of male factor infertility. From clinical view, the main treatment options for infertile males with varicoceles have been varicocelectomy and assisted reproductive techniques (ART). Aim of the study: to assess the role of varicocelectomy in management of male infertility by observation of spermogram changes before and after varicocelectomy. Patients and methods: This study was carried out on 20 male patients attending General Surgery and Dermatology outpatient clinics at AlZahraa University Hospital with a history of primary and secondary infertility after taking informed consent. This study was conducted over a period of 2 years, from August 2010 to August 2012. All patients were subjected to complete history and thorough clinical examinations as well as laboratory investigations including complete blood count (CBC), hormonal profile and multiple semen analysis were done for all patients. Semen analysis for each patient was repeated after varicocelectomy for 3, 6, 9 and 12 months. Also, scrotal color Doppler ultrasound was done for all attendants for confirmation of the diagnosis. Results: Out of 20 patients with primary varicocele, 16 (86.6%) had corrected abnormal semen parameters and their wives get pregnant. 60% of those patients were complaining of primary infertility while the remaining (40%) patients were complaining of secondary infertility. Conclusion: varicocele repair for infertile men with clinically palpable varicocele and at least one or more abnormal semen parameters is recommended as easy and simple treatment of primary or secondary male infertility, and improving fertility rate. [Hamed A. ALBadawy; Doaa Abd El Maleek Pessar, Abeer M. Kamel, Hanan M.A. Darweesh. Spermogram changes and pregnancy rate in Egyptian infertile males before and after varicocelectomy. J Am Sci 2012;8(11):583586]. (ISSN: 15451003). http://www.jofamericanscience.org. 87 Keywords: (ART): assisted reproductive techniques, (CBC): complete blood count, (ROS): reactive oxygen species, (4-HNE): 4hydroxy2nonenal modified proteins, (WHO): world health organization, (GFR-a1): glial cell derived neurotropic factor specific receptor, (8-OHdG): 8hydroxy2deoxyguanosine, (FSH): follicular stimulating hormone, (LH): luteinizing hormone.
Bangladesh Medical Research Council Bulletin, 2018
Prevalence of varicocele is approximately 15.1% of the general population. Studies showed that surgical correction of clinically palpable varicocele could improve the semen parameters. However, there is scarce of study to demonstrate post-operative outcome of varicocelectomy among the patients with abnormal semen parameter. Therefore, this study tried to compare the microscopic changes in sperm concentration, sperm motility, and sperm morphology before and after varicocelectomy. This quasi-experimental study was done in Urology Department of Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh from November 2014 to April 2016. Forty five patients with clinically palpable varicocele were finally includedin the study. Semen parameters of these patients were assessed by following the World Health Organization′s 5th manual of semen parameter, 2010. Subinguinal varicocelectomy was performed in every case. Follow up was done three months and six months after surgery. Dat...
Improvement in semen parameters after varicocoelectomy
International Surgery Journal, 2020
Background: Varicoceles are associated with abnormal sperm parameters. The difference is more marked in cases with infertility and higher grades of varicocele. Following ligation of varicocele, there is a significant improvement in the sperm counts.Methods: The study was conducted in the department of general surgery at Maulana Azad Medical College and associated Lok Nayak Hospital from September 2012 to April 2014. The study was conducted on 40 numbers of patients. These were divided into 2 groups, group 1 (n=20) constituted of patients with varicocele who are symptomatic. Group 2 (n=20) constituted of patients with varicocele who are asymptomatic (infertile). All patients were evaluated by clinical examination and Doppler to grade the varicocele. All patients underwent detailed semen analysis. Patients of both the groups underwent FNAC of bilateral testes. The patients with clinical or Doppler detectable varicocele were taken up for low ligation of varicocele. Patients were follow...
Gynecology and Obstetrics Research - Open Journal, 2015
Varicocele is a disease characterized by abnormal dilatation of the testicular veins within the pampiniform plexus due to difficult blood return in this region. The prevalence of varicocele is 30 to 40% in infertile men, who frequently show seminal changes and a high rate of sperm DNA Fragmentation Index (DFI), one of the conditions responsible for failure of spermatogenesis. Several studies have demonstrated that varicocelectomy may improve the seminal parameters and even the quality of genetic material. However, published papers are predominantly retrospective rather than prospective. In this study, we show some effects of varicocelectomy on the seminal quality and DFI rate of men who were submitted compared to men who did not undergo the procedure, before and after nine months of the intervention. This preliminary data was a prospective, randomized and controlled clinical trial in which 15 men with varicocele and five men without varicocele (control group) were included. In the varicocele group, five patients were submitted to varicocelectomy and 10 were not. The DFI rate was analyzed using the Halosperm G2 ® kit and the seminal parameters were assessed according to the criteria established by the World Health Organization (WHO), 2010. A difference in DFI rate was detected between the varicocele (52% ± 0.28) and the control group (27% ± 0.07). In the varicocele group, no differences in DFI rate were observed between patients submitted to varicocelectomy and patients who were not submitted to the procedure, however, we could observe a sharp increase in the sperm concentration of patients who underwent surgery (28.33 x 10 6 /ml ± 26.91) compared with not submitted varicocelectomy (3.03 x 10 6 /ml ± 14.74). These preliminary results emphasize that men with varicocele have a higher DFI rate.
International Journal of Andrology, 2001
Several theories have been advanced to explain the mechanisms by which varicocele impairs male fertility. These theories include scrotal hyperthermia, retrograde¯ow of adrenal or renal metabolites, Leydig cell dysfunction and hypoxia. Varicocele is reported to be associated with elevated reactive oxygen species (ROS) production in spermatozoa and diminished seminal plasma antioxidant activity. The aim of this study was to investigate whether surgical correction of varicocele might reduce ROS or increase the antioxidant capacity of seminal plasma from infertile patients with varicocele. The study group consisted of 68 infertile males, selected from patients scheduled for varicocelectomy at Cairo University Hospital during the year 1999. Seminal plasma levels of two ROS [malondialdehyde (MDA), hydrogen peroxide (H 2 O 2)] and one ROS radical [nitric oxide (NO)] were estimated as well as six antioxidants [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), vitamin C (Vit C), vitamin E (Vit E), albumin) on the day prior to varicocelectomy. For comparison, the same parameters were measured again 3 and 6 months post-operatively. A statistically signi®cant reduction in the 3 month post-operative levels of MDA, H 2 O 2 and NO was observed when compared with the preoperative values. A further signi®cant reduction took place during the following 3 months. Four of the six antioxidants tested (SOD, CAT, GPx, and Vit C) showed a signi®cant increase in seminal levels when comparing 3-month post-operative with pre-operative values. A further signi®cant increase of the four antioxidant levels took place during the following 3 months. No signi®cant change in the level of seminal plasma albumen took place during the ®rst 3 months after varicocelectomy, however, a signi®cant increase was noted during the next 3 months. In contrast to other antioxidants, seminal plasma levels of Vit E showed a signi®cant decrease when comparing 3-month post-operative with preoperative values. A further signi®cant decrease took place during the following 3 months. It is concluded that varicocelectomy reduces ROS levels and increases antioxidant activity of seminal plasma from infertile men with varicocele.