Development of CD8alpha-Positive Dendritic Cells from a Common Myeloid Progenitor (original) (raw)
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Blood, 2000
Two dendritic cell (DC) subsets have been identified in the murine system on the basis of their differential CD8α expression. CD8α+ DCs and CD8α− DCs are considered as lymphoid- and myeloid-derived, respectively, because CD8α+ but not CD8α− splenic DCs were generated from lymphoid CD4low precursors, devoid of myeloid reconstitution potential. Although CD8α− DCs were first described as negative for CD4, our results demonstrate that approximately 70% of them are CD4+. Besides CD4− CD8α− and CD4+CD8α− DCs displayed a similar phenotype and T-cell stimulatory potential in mixed lymphocyte reaction (MLR), although among CD8α− DCs, the CD4+ subset appears to have a higher endocytic capacity. Finally, experiments of DC reconstitution after irradiation in which, in contrast to previous studies, donor-type DCs were analyzed without depleting CD4+ cells, revealed that both CD8α+ DCs and CD8α− DCs were generated after transfer of CD4low precursors. These data suggest that both CD8α+ and CD8α− D...
Blood, 2002
CD8α+ and CD8α− dendritic cells (DCs) have been considered as independent DC subpopulations both ontogenetically and functionally during recent years. However, it has been demonstrated that both DC subsets can be generated from a single precursor population, supporting the concept that they do not represent separate DC lineages. By using highly purified splenic CD8α− DCs, which were injected intravenously and traced by means of an Ly5.1/Ly5.2 transfer system, this study shows that CD8α− DCs acquired the phenotypic characteristics of CD8α+ DCs, by a differentiation process involving CD8α, DEC-205, and CD24 up-regulation, paralleled by the down-regulation of CD11b, F4/80, and CD4. These data demonstrate that CD8α+DCs derive from CD8α− DCs, and strongly support that CD8α− and CD8α+ DCs represent different maturation or differentiation stages of the same DC population. Therefore, CD8α+ DCs would represent the last stage of DC differentiation, playing an essential role in the induction o...
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