Aspergillus niger Van Tiegh., A Promising Bioprospecting Fungus For The Production of Exopolysaccharide (original) (raw)
Fermentative production of exopolysaccharide by bioprospecting fungus, Aspergillus niger was studied. Sucrose and maltose were used as carbon sources. Polysaccharide production was maximum in 8 & 10% of substrates. Polysaccharide production was increased with increase in growth of mycelium of A. niger in both the substrates. The optimum pH and incubation periods for the maximum production of polysaccharide were pH 7 and 15 days respectively. The polysaccharide produced by A. niger was the polyol of 3,4,6 tri-o-methyl D glucose with β -1-3 glycosidic linkage commonly known as glucan. The polysaccharide produced by this species may be composed of glucose and little amount of galactose and other oligosaccharides. Polarimetric studies revealed that the fractions containing glucose gave laevorotatory values of the optical rotation. Total acidity was maximum in pH 4 and total carbohydrate, reducing sugar was maximum in pH 7, protein and nitrogen values were varied as per experimental conditions.
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