Determination of N-acetylglucosamine in cosmetic formulations and skin test samples by hydrophilic interaction liquid chromatography and UV detection (original) (raw)

Journal of pharmaceutical and biomedical analysis, 2014

Abstract

N-Acetylglucosamine is an ingredient in pharmaceuticals, nutritional supplements and in cosmetics. N-Acetylglucosamine in cosmetics is expected to improve skin hydration, reparation, and to contribute as anti-wrinkle agent. This study reports on the validation and application of an HPLC method based on HILIC and UV detection for determining N-acetylglucosamine in cosmetics and in samples obtained after testing the skin exposed to cosmetics formulations. The chromatographic column used is a ZIC(®)-pHILIC (150mm×4.6mm, 5μm particle size) on which a mobile phase containing acetonitrile-aqueous KH2PO4 (70:30, v/v) 15mM was applied in isocratic elution mode injecting 20μl of sample at 0.5ml/min constant flow-rate and 10±1°C column temperature. Under these conditions the total run time was 10min and N-acetylglucosamine eluted baseline separated from all other compounds in the samples. Calibration in the range from 40 to 80μg/ml allowed to assess the method linearity (R(2)>0.999) in a c...

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