Malaysian Fruit Bats Phylogeny Inferred Using Ribosomal RNA (original) (raw)
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Phylogeny of fruit bats inferred using 12S and 16S rRNA. 2008. Scopus Index
2008
Fourteen species of the Malaysian fruit bats (Pteropodidae) were used in this DNA taxonomy using 1,334bp of the 12S ribosomal RNA (rRNA), transfer RNA (tRNA) valine and 16S rRNA gene segments. Previous studies using DNA found contradictions between morphology and molecular data in inferring the phylogeny of the fruit bats proposed by Andersen (1912). Our phylogenetic analysis using the neighbor-joining and the maximum parsimony methods did not support the monophyly of the subfamily Macroglossinae and the cynopterine group of the subfamily Pteropodinae as proposed by classical taxonomists. This is congruent with previous molecular studies. Here, we provide the first registered 12S rRNA, tRNA valine and 16S rRNA sequence records for Dyacopterus spadiceus in the GenBank database. This study represents the first attempt to infer the phylogenetic relationship of fruit bats from Malaysia using molecular methods.
The taxonomic relationships of the Old World fruit bat family, Pteropodinae, by Anderson (1912) using morphological characters have been challenged by several authors. Previous studies using mitochondrial DNA (mtDNA) found major disagreement between morphology and molecular data in inferring the phylogeny of the fruit bats. Fifty-eight samples from 14 species of the Malaysian fruit bats (Family: Pteropodidae) was used in this study to examine on the phylogenogenetic relationship between species of fruit bats using 395 base pairs of partial mtDNA cytochrome b (cyt b) gene. Our phylogenetic analysis using neighbour-joining and maximum parsimony methods failed to support the monophyly of both the rousettine and cynopterine groups of the Subfamily Pteropodinae.
… and fifty years …, 2005
The taxonomic relationships of the Old World fruit bat family, Pteropodinae, by Anderson (1912) using morphological characters have been challenged by several authors. Previous studies using mitochondrial DNA (mtDNA) found major disagreement between morphology and molecular data in inferring the phylogeny of the fruit bats. Fifty-eight samples from 14 species of the Malaysian fruit bats (Family: Pteropodidae) was used in this study to examine on the phylogenogenetic relationship between species of fruit bats using 395 base pairs of partial mtDNA cytochrome b (cyt b) gene. Our phylogenetic analysis using neighbour-joining and maximum parsimony methods failed to support the monophyly of both the rousettine and cynopterine groups of the Subfamily Pteropodinae.
Phylogenetic Relationships of Fruit Bats (Family: Pteropodidae). 2006
The taxonomic relationships of the Old World fruit bat family, Pteropodinae, by Anderson (1912) using morphological characters have been challenged by several authors. Previous studies using mitochondrial DNA (mtDNA) found major disagreement between morphology and molecular data in inferring the phylogeny of the fruit bats. Fifty-eight samples from 14 species of the Malaysian fruit bats (Family: Pteropodidae) was used in this study to examine on the phylogenogenetic relationship between species of fruit bats using 395 base pairs of partial mtDNA cytochrome b (cyt b) gene. Our phylogenetic analysis using neighbour-joining and maximum parsimony methods failed to support the monophyly of both the rousettine and cynopterine groups of the Subfamily Pteropodinae.
The first mitochondrial genome data of an old world fruit bat, Cynopterus sphinx from Malaysia
Mitochondrial DNA Part B
We assembled the complete mitogenome of Cynopterus sphinx (Vahl, 1797) of the family Pteropodidae originating from Malaysia. The total mitogenome size was 16,710bp which consists of 37 genes (13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one control region). A phylogenetic and BLASTn result showed the mitogenome sequence in this study varies by nearly 7% (93.48% similarity) from the same species in Cambodia. The next closest match of BLASTn was at 92% similarity to the C. brachyotis. This suggests the species-complex in Cynopterus sp. has given rise to the genetic variability.
American Museum Novitates
Old World fruit bats (Chiroptera: Yinpterochiroptera: Pteropodidae) are a diverse radiation endemic to the tropics of Africa, Asia, Australia, and nearby island archipelagos. Recent molecular analyses have provided considerable resolution of phylogenetic relationships within this group, but many points of uncertainty have remained including the position of several enigmatic taxa (e.g., Notopteris, Eidolon), relationships among species in more diverse subfamilies and genera (e.g., Pteropodinae, Pteropus, Epomophorus), and topology of the backbone of the tree. Here we provide a new, synthetic analysis including representatives of all 45 currently recognized genera and enhanced sampling in several speciose genera. Our matrix included four nuclear genes regions (vWF, RAG1, RAG2, and BRCA1) and four mitochondrial gene loci (Cytb, tRNA valine, 12S rRNA, and 16S rRNA) for a total of >8000 bp including new sequence data for 13 species. Maximum likelihood and Bayesian analyses resulted in trees supporting recognition of six main suprageneric clades similar in content to those identified in our previous studies. We did not recover strong support for relationships among the main clades along
2008
Approximately 24 species classified in three groups (Artibeus, Dermanura, and Koopmania) compose Subtribe Artibeina, an assemblage of New World leaf-nosed bats (Phyllostomidae) for which evolutionary relationships have proven difficult to resolve. We examined artibeine systematics through broad taxonomic sampling and phylogenetic analysis of DNA sequences for two mitochondrial genes. Analysis of 16S rRNA sequences offered an additional test of previous genealogical hypotheses, and facilitated knowledge about the congruence in variation between the well studied cytochrome-b gene and the evolutionary history of this complex of bats. Our results illustrate a high degree of congruence between these linked mitochondrial loci that in combination offers a well resolved gene tree and robust predictions to all but a few of the examined relationships. Highlights include: monophyly of Artibeina in contrast to previous hypotheses of polyphyly; two main lineages within Artibeina in accordance with monophyly of the smaller Dermanura species and larger Artibeus species; sister relationship between A. concolor and other Artibeus species rather than with Dermanura, contrasting the argument for recognizing A. concolor as a separate genus (Koopmania); reconfirmation of several species formerly considered subspecies (A. planirostris, A. schwartzi, D. bogotensis, D. rava, and D. rosenbergi); and further indication that A. intermedius and A. lituratus are conspecific.
Journal of Genetic Engineering and Biotechnology
Background Cynopterus brachyotis (Müller 1838) is a generalist and widespread fruit bat species which inhabits different types of habitats in Southeast Asia. This species plays an essential role as a seed disperser and pollinator. Morphological study and phylogenetic analysis using mtDNA markers (cyt-b and D-loop) revealed that this species had two different forms in peninsular Malaysia and Borneo and six lineages in Southeast Asia that lead to new species formation. In addition, this species is also reported to have high genetic diversity in Malaysia and Thailand based on the D-loop sequence. However, a phylogenetic and genetic variation study of C. brachyotis in Indonesia has not been conducted yet. These two studies are important as additional information for taxonomic and population genetic studies of this species. Thus, we performed the phylogenetic and genetic diversity analysis of the C. brachyotis population collected from seven habitats on Java island, including open-fragme...