DEVELOPMENT AND VALIDATION OF A HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD TO DETERMINE VANCOMYCIN CONCENTRATIONS IN PLASMA AND PIG PULMONARY TISSUE (original) (raw)

Journal of Liquid Chromatography & Related Technologies - J LIQ CHROMATOGR RELAT TECHNO, 2012

Abstract

Simple and specific analytical methods were developed and validated to quantify vancomycin in plasma and lung tissue, which were obtained from a model of pneumonia in mechanically ventilated piglets. Tissue and plasma samples were precipitated and centrifuged; 100 µL of the supernatant were injected into the chromatographic system. Ceftazidime was used as the internal standard. The stationary phase was a silica based column Symmetry300 C18 (150 × 4.6 mm) with pre-column. The mobile phase consisted of 20% ultrafiltered water and 80% of (A) 75 mM sodium acetate buffer (pH = 3) with (B) acetonitrile (92%/8%;v/v). Isocratic flow rate was set at 0.8 mL/min and 0.7 mL/min for plasma and tissue samples, respectively. UV absorbance detection was set at 230 nm. Standard curves showed good linearity for plasma and pulmonary tissue (r > 0.99). The lower limits of quantitation were 1.56 µg/mL and 3.13 µg/mL for plasma and tissue, respectively. The intra- and inter-day precisions and accuracy...

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