Callus induction and plant regeneration of tomato (Lycopersicon esculentum cv. IPA 5) via anther culture (original) (raw)
Related papers
2016
The study was conducted to develop an efficient regeneration protocol in tomato through callus induction for subsequent plantlet regeneration. Seeds were inoculated on MS medium where the germination rate was 78.4%. The leaves were used as explants. Different concentration and combination of plant growth regulators (PGRs) were added with MS medium to observe their efficacy on callus induction, shoot initiation and root formation. Leaf explants cultured on MS medium fortified with 3 mg/L BAP gave the highest number of shoots (3.5) at 45 DAC. Among the concentrations of PGRs, 0.25 mg/L IAA produced the highest length (5.149 cm) of plantlets, number (5.5) of leaves and fresh weight (0.781 g) of plantlets with the leaf explants at 45 DAC. The concentration of 0.5 mg/L IAA produced the highest number (25.25) of roots/plantlet, length (8.785 cm) of roots at 45 DAC, from the same explants. The highest survival rate of in vitro regenerated plantlets in the pot was 70.00 % with the leaf expl...
In vitro regeneration protocol development via callus formation from stem explant of tomato
2015
The experiment was conducted on in vitro regeneration of tomato at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University, Patuakhali. The objective was to develop an efficient regeneration protocol in tomato through callus induction for subsequent plantlet regeneration. Seeds were inoculated on MS medium where germination rate was 78.4%. The stems of in vitrocultured seedlings were used as explants. Different concentrations and combinations of growth regulators were added to MS medium to observe their efficacy on callus induction, shoot initiation and root formation. Stem explants cultured on MS medium fortified with 2 mg/L BAPgave the highest number of shoots (3.0) at 45 DAC. Among the concentrations of PGRs, 0.25 mg/L IAA produced the highest length (4.064 cm) of plantlets, number (5.0) of leaves and fresh weight (0.663 g) of plantlets with the stem explants at 45 DAC. The concentration of 0.5 mg/L IAA produced the highest number (21.00) of roots/plantlet, length (7.676 cm) of roots at 45 DAC, from the same explants. The highest survival rate of in vitro regenerated plantlets in the pot was 70.00 % with the stem explants. The results of the current study showed significant increase in the growth of callus of Solanumlycopersicon Mill. Indicating a good efficiency of the optimized media composition and the experimental model used in comparison to other studies of similar nature.
Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiĭskaia akademiia nauk
The influence of explant type as well as of the type of growth regulators and concentration on callus induction processes and somatic organogenesis of shoots was studied in vitro on four tomato genotypes of Russian breeding. Cytological study of callus tissue was conducted. It was established that tomato varieties possess a substantially greater ability to indirect shoot organogenesis compared with the F1 hybrid. The highest frequency of somatic organogenesis of shoots, as well as their number per explant, was observed for most of the genotypes studied during the cultivation of cotyledons on Murashige-Skoog culture medium containing 2 mg/l of zeatin in combination with 0.1 mg/l of 3-indoleacetic acid. An effective protocol of indirect somatic organogenesis of shoots from different explants of tomato varieties with a frequency of more than 80% was developed.
The Effect of Plant Growth Regulators on Callus Induction and Somatic Embryogenesis of Hybrid Tomato
2015
Efficient tissue culture system is important for transformation of important genes in hybrid tomato cultivars. The present study was undertaken to develop an efficient tissue culture system for hybrid tomato cultivar Peto-86. The young primary leaves and stems were inoculated into five different MS media having different concentrations of plant growth regulators in different combinations for callus induction, somatic embryogenesis and for both direct and indirect regeneration. Maximum callus induction frequency 90% was achieved with MS media containing 2,4-D 4 mg L and BAP 0.5 mg L. The direct somatic embryogenesis was found highest on MS media supplemented with 2,4-D 4 mg L and BAP 0.5 mg L. Maximum indirect regeneration frequency 87% was achieved from primary leaves explants with MS media containing IAA 0.5 mg L and BAP 3 mg L and highest direct regeneration frequency 77% was obtained from primary leaves explants with MS media containing NAA 1 mg L and BAP 3 mg L. The high concent...
… of the Crop Science Society of …, 2006
Sixteen tomato genotypes were evaluated for anther culture response at plant tissue culture laboratory, university of Jordan. Anthers containing prophase-I meiotic stage were transferred to MS basal medium supplemented with 20% sucrose, 7 g/l Agar (Bacto) and 0.25 mg/l Zeatin and 0.5 mg/l IAA. Incubation was made at 25 1 o C and 16 h photo period 40 mol/m 2 /s 2 provided by cool white fluorescent lamps. The experimented was conducted on donor plants grown during summer and autumn/winter growing seasons under natural condition. The result showed variability among genotypes in percentage of callus induction; percentage of swollen anthers and number of days to callus induction. Percentage of swollen anthers was well associated with percentage of callus induction. Out of the sixteen genotypes evaluated only eight genotypes gave callus in both seasons. The over all mean of all genotypes for each season indicated that donor plants grown during autumn/ winter growing season gave higher percentage of callus induction than the summer grown donor plants. The autumn/ winter grown donor plants experienced higher relative humidity and lower temperature. These environmental elements might have affect callus induction positively.
African Journal of …, 2010
In vitro culture response was assessed in tomato (Lycopersicon esculentum Mill. c. v. Omdurman) for optimum callus induction and plantlet regeneration. Callus induction was achieved within seven to ten days directly on the cut surfaces of both hypocotyls and cotyledon explants cultured on Murashige and Skoog (MS) basal medium supplemented with -naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), Thidiazuron (TDZ) and benzyl adenine (BA) alone or in different combinations, but not in hormone free-medium. The highest callusing index (5.3) was obtained on hypocotyls explants cultured on MS medium supplemented with NAA at 0.5 mg/l followed by an index of 5.2 obtained from the same explant by using 0.1 mg/l NAA in combination with BAP at 0.5 mg/l. However, for the cotyledon explants, the highest callusing index (4.7) was obtained on MS medium supplemented with NAA at either 2.0 or 3.0 mg/l. After 8 weeks of culture, organogenesis was observed only on the explants cultured on medium containing different concentrations of TDZ alone or in combination with BAP. The best shoot formation (93%) was obtained for cotyledon explant callus induced on MS medium containing TDZ in combination with BAP both at 0.5 mg/l. The highest number (6) of shoot per explant was obtained when cotyledon explant callus was sub cultured on MS medium supplemented with 3.0 mg/l TDZ. Plain half strength of MS was found to be the best rooting medium, however, addition of IAA at 1.0 mg/l and IBA at 2.0 mg/l were found essential to induce highest number of roots (22.1 ± 0.9) and longer roots (11.0 ± 0.3 cm), respectively. This protocol would be useful to create somaclonal variation and utilize transgenic approaches for varietal improvement of tomato.
Optimization of Callus Induction and Shoot Regeneration from Tomato Cotyledon Explants
Plants
Cultivated tomato (Solanum lycopersicum L.) is one of the most important horticultural crops in the world. The optimization of culture media for callus formation and tissue regeneration of different tomato genotypes presents numerous biotechnological applications. In this work, we have analyzed the effect of different concentrations of zeatin and indole-3-acetic acid on the regeneration of cotyledon explants in tomato cultivars M82 and Micro-Tom. We evaluated regeneration parameters such as the percentage of callus formation and the area of callus formed, as well as the initiation percentage and the number of adventitious shoots. The best hormone combination produced shoot-like structures after 2–3 weeks. We observed the formation of leaf primordia from these structures after about 3–4 weeks. Upon transferring the regenerating micro-stems to a defined growth medium, it was possible to obtain whole plantlets between 4 and 6 weeks. This hormone combination was applied to other genotyp...
Tissue culture studies in tomato (Lycopersicon esculentum) var. Moneymaker
Pak. J. Bot, 2010
A protocol was developed for callus induction and regeneration in tomato (Lycopersicon esculentum) var. Moneymaker. Hypocotyl and leaf disc explants of tomato were used as a starting material for callus induction. Explants were cultured on MS medium having different concentrations of hormones. Maximum callogenesis from hypocotyls was obtained on MS medium supplemented with IAA (2 mg/l), NAA (2 mg/l), BAP (5 mg/l) and Kin (4 mg/l). It was 65.2% for hypocotyls. For leaf discs maximum callogenesis was achieved ...
A new approach forin vitro regeneration of tomato plants devoid of exogenous plant growth hormones
Biotechnology Journal, 2006
Many available methodologies for in vitro regeneration of commercial tomato varieties promote not only the production of normal shoots but also individual leaves, shoots without apical meristems and vitrified structures. All these abnormal formations influence and diminish the regeneration efficiency. At the basis of this phenomenon lies callus development. We optimized an alternative procedure by which the regeneration occurs without abnormal shoot formation. The portion including the proximal part of hypocotyls and the radicle was cultured on medium consisting of Murashige and Skoog salts, 4 mg/L thiamine, 100 mg/L mio-inositol and 3% sucrose. After twothree weeks, 60% explants showed adventitious shoot formation. No changes in the morphological characteristics of regenerated plants and fruits were observed as compared with parents. Karyotypic analysis of regenerated plants showed no variations in chromosome number. The optimized procedure offers the advantage of tomato plant regeneration avoiding callus formation, which enables normal plant recovery with an efficiency ranging from 1.45 ± 0.05 to 2.57 ± 0.06 shoots per explant in Campbell-28, Amalia, Lignon, and Floradel cultivars.