Reverse genetics of negative-strand RNA viruses: Closing the circle (original) (raw)

This paper discusses the significant advances in reverse genetics techniques for manipulating negative-strand RNA viruses, which have traditionally been more challenging to engineer than their positive-strand counterparts. Key developments include the ability to recover designer viruses and the construction of gene sequences from historical strains, amid concerns regarding public health risks associated with engineered pathogens. The paper outlines various methods employed for rescuing negative-strand RNA viruses from plasmid DNA, emphasizing the need for responsible practices in this rapidly evolving field.