MMP-9 expression increases according to the grade of squamous intraepithelial lesion in cervical smears (original) (raw)
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MMP-2 AND MMP-9 PROTEIN DETERMINATION IN CERVICAL CANCER SAMPLES
Biolife, 2014
Cancer is characterized by uncontrolled division of cells and the ability of these cells to invade other tissues, either by direct growth into adjacent tissue through invasion or by implantation into distant sites by metastasis. Metastasis is defined as the stage in which cancer cells are transported through the blood stream or lymphatic system, where they continue to proliferate and give rise to secondary tumours'. Cervical cancer is a malignancy of the cervix. About 80% to 90% of cervical cancer and squamous cell carcinomas, which are composed of cells that resemble the flat, thin cells called squamous cells that cover the surface of the endocervix. The remaining 10% to 20% of cervical cancer are adenocarcinomas. Matrix metalloproteinases (MMPs) secreted by cervical and ovarian cancer, especially MMP-2 and MMP-9, play crucial roles in tumor invasion and metastasis. The protein concentrations were found to be 1.84 and 2.48 for normal sample and diseased sample respectively. The molecular weight of MMP-2 and MMP-9 of cancer subjects were determined as 62 KDa and 78 KDa approximately and in normal subjects as 52 KDa and 72 KDa by using SDS-PAGE.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 2015
Matrix metalloproteinases (MMPs) are important enzymes in the tumor microenvironment associated with progression of cervical intraepithelial neoplasia (CIN) towards squamous cell carcinoma (SCC) of the cervix. However, the role of MMPs in the inflammatory process associated with Chlamydia trachomatis infection concomitant with the carcinogenic process driven by HPV has not yet been addressed. In the present study we analyzed the estate of the MMP-9/RECK axis in cervical carcinogenesis. The levels of MMP-9 and RECK expression were analyzed by immunocytochemistry in liquid-based cytology samples from 136 women with high-grade cervical lesions (CIN2/CIN3) and cervical SCC diagnosed by LLETZ, and in 196 women without cervical neoplasia or CIN1. Real-Time qPCR was performed to analyze expression of MMP-9 and RECK in fifteen cervical samples. The presence of HPV-DNA and other genital pathogens was evaluated by PCR. We found a higher expression of MMP-9 (OR=4.2; 95%CI: 2.2-7.8) and lower e...
Uterine cervical neoplasia is a major health problem, and can be a leading cause of death. There is strong evidence that human papillomavirus (HPV) is the principal etiological agent in cervical neoplasia. The objective of this study was to investigate the correlation between HPV-16 and matrix metalloproteinase-9 (MMP-9) and uterine cervical neoplasia. Twenty six formalin fixed, paraffin embedded specimens from patients with uterine cervical neoplasia from teaching laboratories in Baghdad city, were included in this study. In addition fifteen apparently normal cervical tissue blocks have been obtained from patients undergoing hysterectomies for sustained uterine bleeding used as control group. In situ hybridization analysis was performed with cDNA probes to HPV-16 and MMP-9. The expression of HPV-16 and MMP-9 in uterine cervical neoplasia cases in the present study was 50% and 61% respectively, but relationship was not found between expression of HPV-16 and MMP-9 and uterine cervical neoplasia. In conclusion, HPV-16 and MMP-9 may have an essential role in progression of uterine cervical neoplasia.
2012
Uterine cervical neoplasia is a major health problem, and can be a leading cause of death. There is strong evidence that human papillomavirus (HPV) is the principal etiological agent in cervical neoplasia. The objective of this study was to investigate the correlation between HPV-16 and matrix metalloproteinase-9 (MMP-9) and uterine cervical neoplasia. Twenty six formalin fixed, paraffin embedded specimens from patients with uterine cervical neoplasia from teaching laboratories in Baghdad city, were included in this study. In addition fifteen apparently normal cervical tissue blocks have been obtained from patients undergoing hysterectomies for sustained uterine bleeding used as control group. In situ hybridization analysis was performed with cDNA probes to HPV-16 and MMP-9. The expression of HPV-16 and MMP-9 in uterine cervical neoplasia cases in the present study was 50% and 61% respectively, but relationship was not found between expression of HPV-16 and MMP-9 and uterine cervica...
2015
Affiliations: (1) Laboratory of Clinical Cytopathology, School of Pharmaceutical Sciences, University of São Paulo (USP), São Paulo, Brazil. (2) Clinical Cytology Laboratory, State University of Maringá (UEM), Maringá, Paraná, Brazil. (3) Department of Obstetrics and Gynecology, School of Medical Sciences, State University of Campinas, (UNICAMP), Campinas, São Paulo, Brazil. (4) School of Medicine, Santa Casa de São Paulo, INCT-HPV at Santa Casa Research Institute, São Paulo, Brazil. (5) LEMC Laboratory, Discipline of Infectology, UNIFESP, São Paulo, Brazil. Department of Pathology, Santo Amaro University, São Paulo, Brazil (6) Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. (7) Laboratory of Medical Investigation (LIM), Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil; Life and Health Sciences Research Institute, School of Health Sciences, University of Minho, Braga, Portugal; ICVS/3B’s...
Cervical cancer (CC) is the second most frequent neoplasm around the world; and the second cause of death by cancer in Mexican women . It is known that cervical precursor lesions called Low grade Squamous Intraepithelial Lesions (LSIL) or High grade (HSIL) practically present the same risk factors as for CC, by instance the HPV persistent infection, smoking, oral contraceptives consumption, multiparity, etc [2,3].
Cervical cancer (CC) is the fourth most common type of cancer among women and is responsible for about 8% of female cancer deaths worldwide. Understanding how the tumor microenvironment behaves is essential to realize the carcinogenic process, and thus infer possible prognostic biomarkers in the CC development. One of the prognostic factors that has aroused interest in recent years is the increased expression of metalloproteinases (MMPs) in tumor tissues, which is associated with tumor growth and metastasis, and recurrence of degradation of extracellular matrix (ECM) components in tissues of different tumors. Thus, our objective is to evaluate the in situ distribution of MMPs+ cells (MMP-2, -7, -9), as well as TIMP-2+, EphA2+ and EfrinA1+ cells in intraepithelial (IE) and marginal stroma (MS) areas using the technique of immunohistochemistry and immunoscore analysis in low- and high-grade squamous intraepithelial lesions (LSIL and HSIL, respectively) and CC. We observed in MS, a hig...
Cancer, 2000
BACKGROUND. Despite the enormous impact that Papanicolaou (Pap) smear screening has had on the incidence of cervical carcinoma in developed countries, there is still an unacceptably high frequency of occurrence of this cancer. In part, this is due to human error associated with cytologic diagnoses of Pap smears. Also, the use of new sampling devices, such as the cytobrush, has increased the complexity of diagnosing benign and neoplastic cervical cytology. This is particularly apparent in the diagnosis of atypical glandular cells of undetermined significance (AGUS). Approximately 40% of AGUS diagnoses have a corresponding significant lesion at biopsy follow-up, and 60% do not. There is clearly a need for an adjunct to cytologic diagnosis that can readily identify AGUS smears that are diagnostic of significant lesions. The authors have identified the MN/CA9 antigen as a strong candidate for an adjunct biomarker.
Matrix metalloproteinase 1 gene polymorphism as a prognostic predictor of invasive cervical cancer
Gynecologic Oncology, 2005
Objectives. Whereas human papillomavirus (HPV) infection is the major determinant of cervical carcinogenesis, host genetic factors may confer individual susceptibility and prognosis. Matrix metalloproteinase 1 (MMP-1) is an important modulator of carcinogenesis. A guanine insertion (2G) polymorphism at nucleotide À1607 of the MMP-1 gene promoter creates an Ets-1-binding site, which increases transcription activity. The present study investigates the association between MMP-1 polymorphism and cervical neoplasia, and their prognostic significance.