Stability Studies of Twenty-Four Analytes in Human Plasma and Serum (original) (raw)
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Stability of chemical and immunochemical analytes in uncentrifuged plasma samples
Annals of Clinical Biochemistry, 2009
Background: The stability of analyte concentrations in plasma after prolonged contact with blood cells in uncentrifuged lithium-heparin gel tubes was studied. Methods: To investigate the stability of concentrations of 26 chemistry and 15 immunochemistry analytes, the simultaneously drawn samples (n ¼ 50) were measured after 6 h storage at þ88C and þ228C in whole blood and after immediate separation of plasma. The analyte concentrations were measured with a Roche Modular PPEE analyser using reagents from Roche Diagnostics. Results: After prolonged contact with cells a clinically significant change was only observed for potassium where the mean value increased from 4.0 mmol/L to 4.8 mmol/L (P , 0.001) when stored at þ88C. Conclusion: Immediate separation of plasma from cells is recommended. However, when prolonged contact of plasma with cell is unavoidable, samples can be kept uncentrifuged for up to 6 h at þ88C or at þ228C. The stability of potassium, however, is temperature-dependent and cannot be measured from refrigerated blood samples.
Biochemia Medica, 2012
The collected and shipped blood samples are exposed to a various extra-analytical factors prior to analysis. The aim of the study was to determine the stability of analytes in serum gel tubes and plain tubes exposed to a range of storage temperatures and times after centrifugation. Materials and methods: Fifteen healthy volunteers were recruited and venous blood was collected into four tubes, two with and two without gel separator. Analyzing the baseline samples in 30 min, all were stored at 4ºC or 24ºC for 6, 12, 18, 24, 30, 36, 48 and 72 hours and 1 week. Sixteen biochemical anaytes were measured on each sample. Variations remained under the desirable bias considered as clinically insignifi cant. Results: On day three, most analytes remained stable including albumin, protein, creatinine, cholesterol, triglycerides, gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), alanine aminotransferase (ALT), creatine kinase (CK), lactate dehydrogenase (LD) regardless of tube types. Glucose concentration decreased markedly (P = 0.001) beginning from the fi rst hours of storage in plain serum. The stability maximized for the analytes including glucose, total bilirubin, urea nitrogen (BUN), uric acid stored at 4 ºC in gel tubes. Aspartate aminotransferase (AST) activity increased signifi cantly (P = 0.002) up to 48-h, however bias was not signifi cant clinically. High density lipoprotein (HDL) concentration was stable in gel tubes at 24 ºC, in plain tubes at 4 ºC stored up to 36-h. Conclusion: Serum gel or non-gel tubes might be used interchangeably for 11 analytes chilled or at 24 ºC, whereas some restrictions must be applied for glucose, AST, BUN, HDL, and uric acid.
Clinica Chimica Acta, 2008
Aim: With the availability of newer hematology analysers, novel measurement techniques are being introduced into daily routine. As analyte stability may differ according to the employed measurement technique, the aim of this study was to assess the stability of hematologic analytes on different hematology analysers. Methods: We investigated the effect of storage time and storage temperature on sample stability on three newer analytical systems (Advia 120, Bayer Diagnostics; XE 2100, Sysmex and LH 750, Beckman Coulter). Samples were obtained from 64 healthy volunteers and stored at room temperature as well at 4-8°C in order to conduct analysis at different timepoints up to 72 h after blood was drawn. Sample stability was assessed by the graphical truncated normal sequential test. A parameter was considered stable, when its average change was smaller than one coefficient of variation CV (%) of the assessed method, allowing a 5% risk of error.
Sample Management: Stability of Plasma and Serum on Different Storage Conditions
EJIFCC, 2020
Background and objective The analytes stability on serum and plasma are critical for clinical laboratory, especially if there is a delay in their processing or if they need to be stored for future research. The objective of this research was to determine the stability of K3EDTA-plasma and serum on different storage conditions. Materials and methods A total of thirty healthy adults were studied. The serum/plasma samples were centrifuged at 2000g for 10 minutes. Immediately after centrifugation, the serum/plasma analytes were assayed in primary tubes using a Cobas c501 analyzer (T0); the residual serum/plasma was stored at either 2-8°C or -20°C for 15 (T15) and 30 days (T30). Mean concentrations changes in respect of initial concentrations (T0) and the reference change values were calculated. For assessing statistical difference between samples, the Wilcoxon ranked-pairs test was applied. Results We evidenced instability for total bilirubin, uric acid, creatinine and glucose at T15 an...
Effect of delayed centrifugation of whole blood on serum samples stability
La Rivista Italiana della Medicina di Laboratorio - Italian Journal of Laboratory Medicine, 2017
Introduction: Preanalytical conditions are critical for blood sample integrity and poses challenge in surveys involving biochemical measurements. A cross sectional study was conducted to assess the stability of select biomarkers at conditions that mimic field situations in surveys. Material and methods: Blood from 420 volunteers was exposed to 2-8 °C, room temperature (RT), 22-30 °C and > 30 °C for 30 min, 6 hours, 12 hours and 24 hours prior to centrifugation. After different exposures, whole blood (N = 35) was used to assess stability of haemoglobin, HbA1c and erythrocyte folate; serum (N = 35) for assessing stability of ferritin, C-reactive protein (CRP), vitamins B12, A and D, zinc, soluble transferrin receptor (sTfR), total cholesterol, high density lipoprotein cholesterol (HDL), low density lipoprotein cholesterol (LDL), tryglicerides, albumin, total protein and creatinine; and plasma (N = 35) was used for glucose. The mean % deviation of the analytes was compared with the total change limit (TCL), computed from analytical and intra-individual imprecision. Values that were within the TCL were deemed to be stable. Result: Creatinine (mean % deviation 14.6, TCL 5.9), haemoglobin (16.4%, TCL 4.4) and folate (33.6%, TCL 22.6) were unstable after 12 hours at 22-30°C, a temperature at which other analytes were stable. Creatinine was unstable even at RT for 12 hours (mean % deviation: 10.4). Albumin, CRP, glucose, cholesterol, LDL, triglycerides, vitamins B12 and A, sTfR and HbA1c were stable at all studied conditions. Conclusion: All analytes other than creatinine, folate and haemoglobin can be reliably estimated in blood samples exposed to 22-30°C for 12 hours in community-based studies.
Stability of blood analytes after storage in BD SST™ tubes for 12 mo
Clinical Biochemistry, 2009
Objectives: We studied the stability of 33 analytes related to clinical chemistry, bone, and vitamin metabolism, after storage in serum separator tubes (SST™). Design and methods: Blood was collected from 6 subjects using SST tubes. Some serum remained in the tube in contact with the barrier gel and was stored at −80°C for 12 mo. Results: Clinically significant changes occurred only in 1,25-dihydroxyvitamin D and retinol-binding protein. Conclusions: Freezing SST tubes before sample analysis is a viable option for some analytes.
Effect of transport conditions on the stability of biochemical markers in blood
1989
We examined the stability of lipids, carotenoids, a-tocopheroi, and endogenous hormones in plasma prepared from whole blood that had been mailed to a central location for processing. Initially, to simulate transport conditions, wholeblood samples were stored in the laboratory, either at room temperature or cooled, for up to 72 h before processing. in the latter samples, lipid concentrations changed up to 1.4% per day, carotenoids up to -5.5%, and hormones up to 9.5%. In a second study, analyte concentrations in plasma from cooled whole blood mailed via overnight courier were compared with those from plasma that had been immediately separated, frozen, and maiied via overnight courier. Concentrations of cholesterol, high-density lipoprotein subfraction 3, apoiipoprotein B, and retinol were stable. Overall, for each marker except estradiol, the between-person variation was at ieast twice the within-person variation. In a third study, at least 340 zg of DNA was recovered from 30 mL of coolshipped whole blood. Our results indicate that shipping whole-blood samples by overnight courier is feasible for assay of several biochemical markers of interest in epidemiological research.
Stability of common biochemistry analytes in equine blood stored at room temperature
Equine Veterinary Journal, 2009
of blood samples and biochemical analysis of equine blood are unavoidably common in equine practice. The effect that delays may have on the accuracy of results of blood biochemical analyses is not well established. Hypothesis: Delays in processing of blood of up to 72 h results in alterations in measured levels of common biochemical analytes that are of potential clinical relevance. Separation of serum prior to storage is protective against the effects of time delays. Methods: Samples of clotted blood, separated serum and oxalate fluoride plasma from 20 horses were stored and analysed at 0, 24, 48 and 72 h. Graphical exploration of each analyte was undertaken. General linear models with fixed effects were fitted for the whole blood data. The mean bias and 95% limits of agreement were calculated, using bootstrapped data, to assess agreement between pairs of samples analysed at 0 h and other time points. Bland-Altman plots were used to explore general trends in the data. Paired t tests were used to compare the results from whole blood and separated serum. Results: Delays in processing equine blood resulted in significant increases in measured concentrations of aspartate aminotransferase, creatine kinase, lactate dehydrogenase, total bile acids and magnesium. A significant decrease in concentration was identified for glucose (serum and oxalate fluoride preserved plasma). Separation of serum immediately following clot formation resulted in nonsignificant increases in accuracy for some analytes. Conclusions and practical significance: Delays in processing of blood samples may result in biochemical changes of clinical relevance in individual cases; however, in the majority of cases, where delays are only a few days and a number of analytes are assessed concurrently, delays are unlikely to have an effect on the interpretation of results. Separation of serum following clot formation is of limited benefit. Clinical samples in which a delay in processing has occurred may be interpreted with reference to the data presented.