Emergence of Multi Drug Resistant Strains of Eschetichia coli Isolated from Urinary Tract Infection (original) (raw)
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Annali di igiene : medicina preventiva e di comunita, 2019
BACKGROUND Urinary tract infections (UTIs) are a highly prevalent infection among children and Escherichia coli is one of the most important pathogens causing pediatric UTIs. Production of extended spectrum b-lactamase (ESBL) enzymes is an important factor in the emergence of antibiotic resistance among these bacteria. This study aimed to determine the resistance patterns, the frequency of ESBL-encoding genes and the genetic diversity of E. coli strains isolated from children with UTIs who were admitted to children's referral hospital of Hazrat Masoumeh, Qom, Iran. METHODS A total of 102 consecutive non-duplicative strains of E.coli that were isolated from children with UTIs were included into the study. Antibiotic susceptibility of the isolates was determined by disk diffusion method according to the CLSI guidelines. The ability of the isolates to produce ESBLs was phenotypically determined by both combined disk test and double disk synergy test. The ESBL encoding genes (bla CT...
Journal of Coastal Life Medicine, 2016
The study protocol was performed according to the Helsinki declaration and approved by the Research and Ethics Committee of School of Biomedical Laboratory Sciences. Informed written consent was obtained from each subject. The journal implements double-blind peer review practiced by specially invited international editorial board members. Objective: To assess multidrug resistant (MDR) Escherichia coli (E. coli) isolates from patients with urinary tract infection. Methods: From February to June 2014, a cross sectional study was conducted among urinary tract infection patients at the University of Gondar Hospital. Culture and disk diffusion method were used for E. coli isolation and to determine the antibiotic susceptibility patterns. Data were entered and analyzed using SPSS version 20. P < 0.05 was considered as statistically significant. Results: A total of 112 E. coli isolates were identified and the rate of isolation was higher among female participants (28.7%; P = 0.03). Of the isolates, 104 (92.9%) were MDR E. coli; and the isolates showed high resistance rates towards ampicillin (99%), cotrimoxazole (69%), chloramphenicol (58.7%), gentamycin (56.7%) and ceftazidime (55.8%). However, comparative isolates showed low resistance rates to ciprofloxacin (1%), cefepime (8.7%), and ceftriaxone (11.5%). Moreover, resistance rates of MDR E. coli isolates were significantly higher than non-MDR strains for ceftazidime (55.8% versus 12.5%; P = 0.015), and ampicillin (99% versus 87.5%; P = 0.018). Conclusions: High prevalence of MDR E. coli isolates was observed in this study. Regular monitoring of antibiotic resistance rates is necessarily required to improve and revise empirical antibiotic therapy protocols.
IOSR Journals , 2019
This study was designed to investigate the distribution and antimicrobial resistance in urinary tract pathogens, primarily Escherichia coli, in two age groups, children 1-16 years and adults 17-55 y, over a period of 6 months at Al-Thawra Central Educational Hospital-El-Beida City. A total of 265 mid-stream urine samples were collected and cultured, 99 (37.4%) samples isolated with E.coli growth and the remaining 166 (62.6%) samples were negative(found be sterile or another bacteriuria). E. coli was the most common pathogen in both age groups. E. coli resistance to ampicillin and trimethoprim was higher in children than in adults. Resistance to Colistin Sulphate, tobramycin and Cefamandole were higher in children than in adults. Levofloxacin, Ciprofloxacin, Azithromycine and imipenem were found sensitivity in both groups. This data was described E.coli as the common causing of UTI and it showed the resistance of E.coli to several antibiotics. Depend on this study, the appropriate antibiotics for UTI are levofloxacin, ciprofloxacin.
Immunology and Infectious Diseases
Urinary tract infections (UTIs) are serious health affecting problems worldwide. Urinary tract infection (UTI) can be caused by a lot of bacteria. Among them, E. coli is one of the main causes of both nosocomial and community acquired Urinary tract infections in humans. Drug resistance of E. coli is becoming alarming with the passage of time worldwide in general and in third world countries in particular. The study was intended to collect and analyze the samples from the patients suffering from Urinary tract infection of two medical of southern region of Bangladesh to reveal the antibiotic resistance pattern of E. coli responsible for Urinary tract infection. 50 samples from each hospital were taken. 36 and 41 samples respectively were identified as E. coli. Antibiotic sensitivity testing was done on Mueller-Hinton agar by Kirby-Bauer disc diffusion method. All samples exhibited a different percentage of resistance where ciprofloxacin was showed evidence of higher resistance rate, which was 63.88% and 70.73% respectively. Higher resistance (63% & 75%) against Ampicillin was also found in patients of both medical. On the other hand, 16% resistance against ceftriaxone and 23.53% resistance against cefotaxime suggesting, they were comparatively more effective.
Journal of Pharmaceutical Research International, 2020
Background and Objectives: Urinary tract infection (UTI) is one of the most frequent infectious diseases which is caused by Gram-negative bacteria especially Escherichia coli. Multiple resistance to antimicrobial agents are increasing quickly in E. coli isolates and may complicate therapeutic strategies for UTI. The propose of this study was to determine the antibiotic resistance patterns and the multidrug-resistance (MDR) phenotypes in uropathogenic E. coli (UPEC). Materials and Methods: A total of 153 UPEC isolates were collected from both hospitalized patients (95 isolates) and outpatients (58 isolates) from March to October 2018. In order to determine the MDR among UPEC isolates, we have tested 15 antimicrobial agents on Muller Hinton agar by the disk diffusion method. Results: The percentage of MDR isolates (resistant to at least three drug classes such as fluoroquinolones, penicillins and cephalosporins) was 55.5% in the hospitalized patients and the outpatients. Antibiotic re...
Journal of entomology and zoology studies, 2019
Escherichia coli cause Urinary tract infection (UTIs) throughout the world, so to improve infection control measurement knowledge about the distribution and frequency of Urinary tract infection (UTI) is very important. A total of 70 patients were examined of different ages and sexes suffering from UTIs. The isolated bacteria were recognized by conventional biochemical test and the antimicrobial susceptibility tests are performed on Mueller-Hinton agar using disk diffusion (Kirby Bauer's) technique. Out of them UTI’s prevalence in female was 68% and male 32%, the maximum UTIs prevalence rate within the age of 21-40 was (30%), followed by above 80 (07%). E. coli was sensitive to antibiotics (TZP, FOS, F, IMP, AK and SCF) in males 61-80 (09 patients), followed by age 01-20 (09 patients) in the female. However, E. coli show resistant to antibiotic found in male between the age of 61-80 (09 patients), followed by 41-60 (04 patients), while in female, consequently, from the age of 21-...
Bioinformation, 2014
Drug resistance is becoming alarming with the passage of time worldwide in general and in third world countries in particular. Human urine specimens of patients of urinary tract infection at Sheikh Zayed hospital, Lahore, Pakistan were analyzed for drug resistance in Escherichia coli. A total of 69 Escherichia coli isolates from human urine specimens were obtained and screened for their antibiograms. A total of seven antibiotic resistance profiles were obtained with over 65% of the isolates showing multi-drug resistance. Very high resistance levels were detected against augmentin and gentamicin (87.5 &77.5 % respectively) while imipenem and tazocin recorded the least resistance levels (32.5% and 12.5% respectively) among the isolates.
International Journal of One Health, 2021
Background and Aim: Uropathogenic Escherichia coli (UPEC) is commonly involved in urinary tract infections (UTIs), which are generally treated with antibiotics. However, the emergence of multidrug-resistant (MDR) strains of UPEC has made the treatment difficult. There is thus a need to continuously assess their sensitivity to antibiotics. This study aimed to determine the antibiotic resistance patterns and MDR phenotypes of UPEC strains isolated from children diagnosed with UTIs at the Russian Children's Clinical Hospital in Moscow, Russia. Materials and Methods: Kirby–Bauer's disc diffusion method was used to study the sensitivity to antibiotics of 106 UPEC isolates from urine specimens from children (aged from 9 months to 18 years old) diagnosed with UTIs. The results were interpreted in accordance with the Clinical and Laboratory Standards Institute guidelines and the correlations of variables with the degree to which each antibiotic inhibited the UPEC strains in terms of diameter on the disc were determined using Spearman's rank correlation test. A t-test and principal component analysis were performed to visualize the correlations of the susceptibility of UPEC to antibiotics with the age and sex of the patients. Statistical significance was set at p≤0.05. Results: Among the 106 UPEC strains tested, none (0%) showed resistance to fosfomycin (FO), while 84 (79.2%) were resistant (R) to at least one antibiotic. The highest rates of resistance were observed to amoxicillin (69.8%), ampicillin (62.3%), cefazolin (39.6%), trimethoprim (TR) (37.7%), ceftriaxone (34.9%), and tetracycline (33.0%). Interestingly, 22 (20.8%) strains were R to imipenem. UPEC isolates from males aged 1-6 years were more R to antibiotics than those from the other groups, with the exception of TR, to which UPEC isolates from females aged 13-18 years old were less sensitive (S). The multidrug-resistance (MDR) index ranged between 0.00 and 0.75 and we found that more than a quarter of UPEC (31/106) had an MDR index ≥0.5 and only 22 (20.7%) strains were S to all antibiotics tested (MDR index=0). Finally, Spearman's rank correlation test showed that, with the exception of FO, there were correlations between the inhibition diameters of all other antibiotics. Conclusion: FO is the only antibiotic to which all UPECs were S and may be suggested as the first line of treatment for UPEC. Further research is needed to continue monitoring antibiotic resistance and to investigate the genetic features associated with such resistance observed in this study.
Pakistan Journal of Medical and Health Sciences, 2022
Multidrug-resistant Escherichia coli have become a main public health distress in Pakistan and many countries, causing failure in treatment with the resultingenormous health burden. The current study was aimed to find the prevalence of E. coli among clinical isolates and their antibiotics susceptibility pattern using standard procedures. The Purpose of the present study to investigate the Prevalence and antibiotic susceptibility pattern of E. coli detected from clinical patients visiting Khyber teaching hospital Peshawar. In this study a total 415 sample were isolated from the patient of Khyber teaching hospital (KTH) Peshawar. The collected samples were processed for identification including Gram staining and biochemical test. Furthermore, the antibiotic susceptibility pattern of all the positive strains of E. coli was tested using selected antibiotic discs by disc diffusion method as per CLSI guidelines 2019.A total of 415 samples, 112 clinical isolates yielded the growth of E. co...
The prevalence and antibiotic susceptibility pattern of E. coli was analyzed in urine samples of patients with acute and recurrent urinary tract infection visiting a diagnostic laboratory in Lahore. The prevalence of MDR strains of E. coli among the isolates was also determined. A total of 310 isolates (73.1%) of E. coli were obtained from 424 positive culture isolates. The highest percentage of the isolates was present in the age group of 51-75 years in both males and females (30.9% and 9.6% respectively). Females were more frequently affected (77.4%) as compared to males (22.5%, P < 0.02). E. coli showed highest sensitivity towards Imipenem (100%), Meropenem (99.3%), Piperacillin/Tazobactum (96.8%), Amikacin (89.7%), Cefoperazone/Sulbactum (86.8%), Ceftazidime (77.2%), Gentamicin (73.2%), and Aztreonam (67.1%). 65.5% of isolates were resistant to more than 8 drugs, belonging to 3 or more than 3 different classes of drugs.