Analysis of genetic diversity of Russian regional populations based on STR markers used in DNA identification (original) (raw)
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Y-chromosome STR variation in Ukrainian populations
Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology, 2015
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Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology, 2021
The object of the study was sires of the Black-and-White and Holstein breeds, which had STR profiles and passed the genotyping procedure for SNP markers. The subject of the study was the level of genomic inbreeding and homozygosity, as well as population-genetic characteristics based on them. With an increase in Fx based on pedigree data, an increase in the level of genomic inbreeding (FROH) calculated from SNP markers was also noted. The results for 9 STR markers record a wavelike increase in homozygosity from group I to group IV with a subsequent increase from group V to group VII inclusive. During the study of the average FROH values in accordance with the years of birth of sires, a significant difference was found between the last two groups (2009-2011; 2012-2014) from all the others in a pairwise comparison. The results of calculating the FROHand Ca9 of bulls from different countries of origin indicate a statistically significant differentiation of the group of animals from Rus...
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В данном сообщении вводятся в научный оборот Y-STR гаплотипы узбеков, уйгуров, таджиков, пуштунов, хазарейцев, моголов из базы данных Family Tree DNA. 37 гаплотипов были исследованы авторами статьи. Остальные 81 гаплотип публиковались ранее другими авторами, либо лежат в открытом доступе в базах данных Family Tree DNA. В статье исследованы также данные по роду барлас, разные части которого относятся к разным этносам (узбеки, моголы). Аргументирована мысль об изначальной принадлежности барласов к субкладу С2-F4002 (старкластер). Ниже, в Приложении 1, приведены гаплотипы, использованные в этой работе.
The application of microsatellite markers for estimation of genetic diversity of European spruce
Proceedings of the Saint Petersburg Forestry Research Institute, 2018
The review discusses trends in using of microsatellite markers for evaluation of the genetic diversity in European spruce populations. A brief historical outline of SSR marker appearance, development and using for population genetic studies of spruce species is given. The characteristics of groups of the most frequently used microsatellite markers of spruce are recited. The principles of the development of microsatellite multiplexes are described. The published multiplexes of microsatellite markers proposed for estimation of the genetic variability of European spruce are listed. Positive properties and disadvantages of a number of proposed multiplexes and individual loci are noted. Microsatellites from expressed sequence tags (EST-SSR) are highly-valued and they allow us to reveal the genetic diversity in functionally important parts of the genome. EST-SSRs and chloroplast microsatellites (cpSSRs) are characterized by a lower mutation rate than nuclear microsatellites (nSSR) from genomic libraries and they can be more easily applied for related tree species studies. Several kinds of multiplexes containing two or three pairs of WS EST-SSR primers are published. The loci WS0023.B03, WS0022.B15, WS0016.O09, WS0092.A19, WS0073.H08, WS00111.K13, WS0092.M15 developed by D. Rungis and co-authors are the most popular. Despite the fact that a large number of multiplexes of nuclear microsatellites have been published, the analysis of presented data shows that the SSR-marker panels are still in the testing stage and are not ready-made recommended tools for spruce population studies. The optimizing of the panels of microsatellite markers, specifying the composition and the number of loci suitable for assessing the genetic diversity of European spruce remains relevant at the moment.