Strategic manipulation of an industrial biocatalyst - evolution of a cephalosporin C acylase (original) (raw)
Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity
Elena Rosini
Biochemical Journal, 2013
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Mutational Analysis of a Key Residue in the Substrate Specificity of a Cephalosporin Acylase
Robbert Cool, Charles F Sio, Linda G Otten
Chembiochem, 2004
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Altering the substrate specificity of cephalosporin acylase by directed evolution of the-subunit
Robbert Cool
2002
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Altering the Substrate Specificity of Cephalosporin Acylase by Directed Evolution of the beta -Subunit
Robbert Cool, Charles F Sio, Linda G Otten
Journal of Biological Chemistry, 2002
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Evolution of an acylase active on cephalosporin C
Elena Rosini, Giorgia Letizia Marcone, Walter Cabri
Protein Science, 2005
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Immobilization of the acylase from Escherichia coli on glyoxyl-agarose gives efficient catalyst for the synthesis of cephalosporins
Andrés Alcántara
Enzyme and Microbial Technology, 2008
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Biosynthesis of Cephalosporin-C Acylase Enzyme: Optimal Media Design, Purification, and Characterization
Kumar Gaurav
Artificial Cells, Blood Substitutes, and Biotechnology, 2010
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Evaluation of Different Glutaryl Acylase Mutants to Improve the Hydolysis of Cephalosporin C in the Absence of Hydrogen Peroxide
Lorena Betancor
Advanced Synthesis & Catalysis, 2008
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Analysis of a substrate specificity switch residue of cephalosporin acylase
Robbert Cool, Charles F Sio, Linda G Otten
Biochemical and Biophysical Research Communications, 2003
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One-pot conversion of cephalosporin C by using an optimized two-enzyme process
Elena Rosini, Gianluca Conti
Catal. Sci. Technol., 2015
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Affinity labelling of cephalosporin C acylase from Pseudomonas sp. N176 with a substrate analogue, 7β-(6-bromohexanoylamido)cephalosporanic acid
Masami Niwa
Journal of Fermentation and Bioengineering, 1994
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Enzymatic synthesis of cephalosporins. The immobilized acylase from Arthrobacter viscosus : A new useful biocatalyst
Massimo Pregnolato, Teodora Bavaro
Applied Microbiology and Biotechnology, 2007
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A novel 7-β-(4-carboxybutanamido)-cephalosporanic acid acylase isolated from Pseudomonas strain C427 and its high-level production in Escherichia coli
Masami Niwa
Journal of Fermentation and Bioengineering, 1994
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A highly active adipyl-cephalosporin acylase obtained via rational randomization
Robbert Cool, Charles F Sio, Linda G Otten
Febs Journal, 2007
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Characterization and Study of the Orientation of Immobilized Enzymes by Tryptic Digestion and HPLC-MS: Design of an Efficient Catalyst for the Synthesis of Cephalosporins
Daniel Ubiali
Biomacromolecules, 2010
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Cephalosporin C acylase from Pseudomonas species: Production and enhancement of its activity by optimization of process parameters
Arpana Jobanputra
Biocatalysis and Agricultural Biotechnology, 2015
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Selection of multiple mutated cephalosporin acylases with modified substrate specificity
Robbert Cool, Charles F Sio, Linda G Otten
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The 2.0 Å Crystal Structure of Cephalosporin Acylase
Ki-Hong Yoon
Structure, 2000
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Cephalosporin C acylase: dream and(/or) reality
Elena Rosini
Applied Microbiology and Biotechnology, 2013
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One-Pot Chemoenzymatic Synthesis of 3‘Functionalized Cephalosporines (Cefazolin) by Three Consecutive Biotransformations in Fully Aqueous Medium
Massimo Pregnolato
Journal of Organic Chemistry, 1997
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Biotransformation of cephalosporin C to 7-aminocephalosporanic acid with coimmobilized biocatalyst in a batch bioreactor
Eva Michalkova
Bioprocess Engineering, 1996
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Catalytic Properties of d-Amino Acid Oxidase in Cephalosporin C Bioconversion: A Comparison between Proteins from Different Sources
Silvia Sacchi
Biotechnology Progress, 2008
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Overproduction and Purification of Glutaryl 7Amino Cephalosporanic Acid Acylase
Guoping ZHAO
Protein Expression and Purification, 1998
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Expression of GLUTARYL7-AMINOCEPHALOSPORANIC Acid Acylase in Escherichia Coli BL21(DE3) and Immobilization of Recombinant Enzyme on Nanoporous Materials
Le Gia Hy
Vietnam Journal of Science and Technology, 2018
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Comparative characterization of new glutaryl 7-ACA and cephalosporin C acylases
Hitoshi Kojo
Journal of Fermentation and Bioengineering, 1992
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Molecular cloning and characterization of the alkaline ceramidase from Pseudomonas aeruginosa PA01
Maarten Egmond
2003
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