Bio-Photonic Sensing Cells over transparent substrates for anti-gestrinone antibodies biosensing (original) (raw)

Label-free biosensing by means of periodic lattices of high aspect ratio SU-8 nano-pillars

Biosensors and Bioelectronics, 2010

We developed biophotonic sensing arrays of 60 × 60 m 2 made of periodic lattices of high aspect ratio SU-8 nano-pillars in order to demonstrate their capability for label-free molecule detection, as well as the sensitivity enhancement in comparison with a single layer of SU-8. The biophotonic sensing arrays, that we call BICELLs (Biophotonic sensing cells), are interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR). We monitored the surface immobilization of bovine serum albumin (BSA) antigen and anti-BSA antibody (aBSA) recognition. The bioassay exhibits a limit of detection (LOD) in the order of 2 ng/ml limited by the wavenumber uncertainty during the interrogation process. We also estimated and compared the theoretical biolayer thickness with previous results.

Review of Recent Advances in Biosensing with Photonic Crystal Surfaces

IEEE Sensors Journal, 2015

Photonic crystal surfaces that are designed to function as wavelength-selective optical resonators have become a widely adopted platform for label-free biosensing, and for enhancement of the output of photon-emitting tags used throughout life science research and in vitro diagnostics. While some applications, such as analysis of drug-protein interactions, require extremely high resolution and the ability to accurately correct for measurement artifacts, others require sensitivity that is high enough for detection of disease biomarkers in serum with concentrations less than 1 pg/ml. As the analysis of cells becomes increasingly important for studying the behavior of stem cells, cancer cells, and biofilms under a variety of conditions, approaches that enable high resolution imaging of live cells without cytotoxic stains or photobleachable fluorescent dyes are providing new tools to biologists who seek to observe individual cells over extended time periods. This paper will review several recent advances in photonic crystal biosensor detection instrumentation and device structures that are being applied towards direct detection of small molecules in the context of high throughput drug screening, photonic crystal fluorescence enhancement as utilized for high sensitivity multiplexed cancer biomarker detection, and label-free high resolution imaging of cells and individual nanoparticles as a new tool for life science research and single-molecule diagnostics.

Recent Advances in Biosensing With Photonic Crystal Surfaces: A Review

IEEE Sensors Journal, 2016

Photonic crystal surfaces that are designed to function as wavelength-selective optical resonators have become a widely adopted platform for label-free biosensing, and for enhancement of the output of photon-emitting tags used throughout life science research and in vitro diagnostics. While some applications, such as analysis of drug-protein interactions, require extremely high resolution and the ability to accurately correct for measurement artifacts, others require sensitivity that is high enough for detection of disease biomarkers in serum with concentrations less than 1 pg/ml. As the analysis of cells becomes increasingly important for studying the behavior of stem cells, cancer cells, and biofilms under a variety of conditions, approaches that enable high resolution imaging of live cells without cytotoxic stains or photobleachable fluorescent dyes are providing new tools to biologists who seek to observe individual cells over extended time periods. This paper will review several recent advances in photonic crystal biosensor detection instrumentation and device structures that are being applied towards direct detection of small molecules in the context of high throughput drug screening, photonic crystal fluorescence enhancement as utilized for high sensitivity multiplexed cancer biomarker detection, and label-free high resolution imaging of cells and individual nanoparticles as a new tool for life science research and single-molecule diagnostics.

Chapter 5 New Insights on Optical Biosensors : Techniques , Construction and Application

2017

Since Clark ́s enzymatic electrode in 1962s [1], biosensors have been proposed for a range of application and, aiming clinical analysis application, the amperometrical, potentiometrical and optical are the biosensors which have achieved most significant development. In con‐ cept, optical biosensors are those based on the detection of changes on absorption of UV/ visible/Infrared light when chemical reactions occur or on the quantity of light emitted by some luminescent process. Regarding to supramolecular nanostructures and their ability of enhancing the sensing activity when applied to biosensors construction, a very instigating work, presented by Jin Shi et al. [2] showed a way of turning carbon nanotubes into more water-soluble compounds and, consequently, more biocompatible by modifying their sur‐ face with a synthetic DNA sequence. In this way, the carbon nanotubes can overlay the bio‐ sensor electrode more efficiently, enhancing the biosensing activity. Lieden et al. [3] also ...

Ultrasensitive On-Chip Immunoassays with a Nanoparticle-Assembled Photonic Crystal

ACS Nano, 2012

Electrophoretic particle entrapment system (EPES) is employed to generate 2D array of nanoparticles coated with biological molecules (i.e. antibodies). Phase matching of the excitation and the emission in the 2D arrays with particles produces a highly enhanced fluorescence signal that was shown to improve the limit of detection in immunoassays. The phase matching is achieved when the particle are in the sub-100 nm range. A comparison between different size particles shows that the sensitivity of an immunoassay is extended to a range that is difficult to achieve with standard technology (e.g. Enzyme-linked immunosorbent assay-ELISA). The effectiveness of this novel configuration of particle-in-a well was demonstrated with an assay for human epidermal growth factor receptor 2 (HER2; breast cancer biomarker), with a detection limit as low as 10 aM in less than 10 μl of serum-based sample. The limit of detection of HER2 indicated far superior assay performance compared to the corresponding standard 96-well platebased ELISA. The particle-based photonic platform reduces the reagent volume and the time for performing an assay in comparison to competing methods. The simplicity of operation and the level of sensitivity demonstrated here can be used for rapid and early-stage detection of biomarkers.

Integrated optical devices for lab-on-a-chip biosensing applications

Laser & Photonics Reviews, 2012

The application of portable, easy-to-use and highly sensitive lab-on-a-chip biosensing devices for real-time diagnosis could offer significant advantages over current analytical methods. Integrated optics-based biosensors have become the most suitable technology for lab-on-chip integration due to their ability for miniaturization, their extreme sensitivity, robustness, reliability, and their potential for multiplexing and mass production at low cost. This review provides an extended overview of the state-of-the-art in integrated photonic biosensors technology including interferometers, grating couplers, microring resonators, photonic crystals and other novel nanophotonic transducers. Particular emphasis has been placed on describing their real biosensing applications and wherever possible a comparison of the sensing performances between each type of device is included. The way towards achieving operative lab-on-a-chip platform incorporating the photonic biosensors is also reviewed. Concluding remarks regarding the future prospects and potential impact of this technology are also provided.

Micro- and Nanotechnology in Biosensor Research

Chimia International Journal For Chemistry, 1999

Biosensor research is strongly interdisciplinary as it requires experience in chemistry, biochemistry, biology, material science, electronics and engineering. The recent progress in micro-and nanotechnology allows to miniaturize complex systems as well as to address problems at a molecular level. The architecture and even the function of single molecules on a sensor surface have been investigated and can to some extent even be predetermined. At present, microtechnology is well established in the production of micro-fluid transport systems and has a high potential for cell-culturing and monitoring devices in the future. Three different running projects are presented which illustrate the usefulness of micro-and nanotechnology for biosensor research: 1)Investigations on amperometric immunosensor devices, 2) the measurement of binding forces of individual antigen-antibody pairs, and 3) the fabrication of microchannels suitable for neuron-cell growth and recording. Big efforts, however, will be required to integrate the recognition element of a sensor into a device for an intended application