Magnetite Nanostructures as Novel Strategies for Anti-Infectious Therapy (original) (raw)
Related papers
Rasayan Journal of chemistry, 2020
In the present paper the synthesis of magnetite nanoparticles and their surface functionalization with protein has been described. Surface functionalization of these nanoparticles was confirmed using various techniques including Transmission Electron Microscopy, X-Ray Diffraction, Vibrating Scanning Magnetometry, Thermo-gravimetric studies and Fourier Transform Infrared Spectroscopy. Further, surface-functionalized magnetite nanoparticles were loaded with ciprofloxacin drug and then screened for their antibacterial activity against two gram-positive (Bacillus subtilis and Staphylococcus aureus) and two gram-negative (Pseudomonas aeruginosa and Escherichia coli) bacterial strains.
International Journal of Pharmaceutics, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Anaerobe, 2013
A new water-dispersible nanostructure based on magnetite (Fe 3 O 4 ) and usnic acid (UA) was prepared in a well-shaped spherical form by a precipitation method. Nanoparticles were well individualized and homogeneous in size. The presence of Fe 3 O 4 @UA was confirmed by transmission electron microscopy, Fourier transform-infrared spectroscopy, and X-ray diffraction. The UA was entrapped in the magnetic nanoparticles during preparation and the amount of entrapped UA was estimated by thermogravimetric analysis. Fabricated nanostructures were tested on planktonic cells growth (minimal inhibitory concentration assay) and biofilm development on Gram-positive Staphylococcus aureus (S. aureus), Enterococcus faecalis (E. faecalis) and Gram-negative Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa) reference strains. Concerning the influence of Fe 3 O 4 @UA on the planktonic bacterial cells, the functionalized magnetic nanoparticles exhibited a significantly improved antimicrobial activity against E. faecalis and E. coli, as compared with the Fe 3 O 4 control. The UA incorporated into the magnetic nanoparticles exhibited a very significant inhibitory effect on the biofilm formed by the S. aureus and E. faecalis, on a wide range of concentrations, while in case of the Gramnegative microbial strains, the UA-loaded nanoparticles inhibited the E. coli biofilm development, only at high concentrations, while for P. aeruginosa biofilms, no inhibitory effect was observed. The obtained results demonstrate that the new water-dispersible Fe 3 O 4 @UA nanosystem, combining the advantages of the intrinsic antimicrobial features of the UA with the higher surface to volume ratio provided by the magnetic nanocarrier dispersible in water, exhibits efficient antimicrobial activity against planktonic and adherent cells, especially on Gram-positive strains.
Biocompatible hydrodispersible magnetite nanoparticles used as antibiotic drug carriers
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2015
Here we report a newly synthesized vectorizing nanosystem, based on hydrodispersible magnetite nanoparticles (HMNPs) with an average size less than 10 nm, obtained by precipitation of Fe(II) and Fe(III) in basic solution of p-aminobenzoic acid (PABA), characterized by high-resolution transmission electron microscopy (HR-TEM), dynamic light scattering (DLS), X-ray diffraction (XRD), differential thermal analysis coupled with thermogravimetric analysis (DTA-TGA) and bioevaluated for cytotoxicity and antibiotic delivery in active forms. The obtained data demonstrate that HMNPs can be used as an efficient drug delivery system, for clinically relevant antimicrobial drugs. HMNPs antimicrobial activity depended on the loaded drug structure and the tested microbial strain, being more efficient against Pseudomonas aeruginosa, comparing with the Escherichia coli strain. The novel HMNPs demonstrated an acceptable biocompatibility level, being thus a very good candidate for biomedical applicati...
Journal of Molecular Liquids, 2018
Magnetite has shown some promise as a biomedical material and antibacterial agent; however the benefits are normally only realized when it is used in combination with other metals or drugs. Unfunctionalized magnetite may be a biocompatible alternative. This report discusses the synthesis and potent antibacterial activity, with low associated mammalian organ toxicity, of nanomagnetite particles. Magnetite (Fe3O4) nanoparticles were electrochemically prepared in a green surfactant-free, closed water loop system. These materials, characterized by X-ray diffraction, FTIR, and vibrational magnetometry, also appear contaminated with Fe-O-O-H functionalities. This physical characterization is accompanied by a computational investigation of truncated clusters showing that a magnetite-derived cluster of 7 iron atoms is a sufficient model to generate the vibrational frequencies experimentally observed in magnetite using DFT calculations. The nanoparticles, evaluated for antibiotic activity, were shown to have minimum inhibitory concentrations of 2.8 and 2.0 µg/mL against E. coli and S. aureus respectively. This is both a 100-fold lower concentration than the human cytotoxic dose determined by an MTT assay and is 3 also comparable to the effective dose of traditional antibiotics. A dose-dependent decrease in catalase activity and an increase in the levels of lipid peroxidation suggests that these nanoparticles act through damaging the anti-oxidant systems in cells. However, renal and hepatic damage was only observed at daily doses (2 weeks) of 100 g/mL and higher. This significant therapeutic window suggests that these materials might prove useful as potential complementary therapeutics in the future.
Water soluble magnetite nanoparticles for antimicrobial drugs delivery
Water-soluble magnetite has been prepared through precipitation approach. These nanoparticles coated with sulfanilic acid could be dispersed in hydrated aqueous systems. The product was characterized with X-ray powder diffraction (XRD), Dynamic Light Scattering (DLS) and the in vitro efficacy as antibiotic delivery vehicles as well as their influence on the eukariotic cells. The XRD pattern confirm the product to be Fe 3 O 4. The nanoparticles with average size 10.45 nanometers are not cytotoxic and do not influence the eukariotic HeLa cell cycle, representing potential tools for the delivery of drugs in a safe manner. Water soluble magnetite improves the activity of currently used antibiotics, representing potential as a nanocarrier for these antimicrobial substances, to achieve extracellular and intracellular targets.
Functionalized Magnetic Nanoparticles and Their Effect onEscherichia coliandStaphylococcus aureus
Journal of Nanomaterials, 2015
Magnetite (Fe3O4) nanoparticles were prepared using coprecipitation and subsequently surface-functionalized with 3-aminopropyltriethoxysilane (APTS), polyethylene glycol (PEG), and tetraethoxysilane (TEOS). Nanoparticle morphology was characterized using scanning electron microscopy, while structure and stability were assessed through infrared spectroscopy and zeta potential, respectively. Average size of the nanoparticles analysed by dynamic light scattering was 89 nm, 123 nm, 109 nm, and 130 nm for unmodified magnetite and APTS-, PEG-, and TEOS-modified magnetite nanoparticles, respectively. Biological effect was studied on two bacterial strains: Gram-negativeEscherichia coliCCM 3954 and Gram-positiveStaphylococcus aureusCCM 3953. Most of modified magnetite nanoparticles had a significant effect onS. aureusand not onE. coli, whereas PEG-magnetite nanoparticles displayed no significant effect on the growth rate of either bacteria.
Preparation and characterization of magnetic nanoparticles loaded with antimicrobial agent
Journal of Contemporary Pharmacy
Background: Bacterial infections are an important cause of serious health issues worldwide. Various antibacterial drugs have been developed but they have numerous side effects. Development of drug loaded magnetic nanoparticles will help to achieve targeted drug delivery while sustaining the release of drug. It will also enhance its antibacterial activity by using iron oxide. Method: Drug loaded iron oxide nanoparticles were developed to sustain and enhance the antibacterial activity of drug. Chitosan was used as a polymer. The method adopted to prepare magnetic nanoparticles was co-precipitation. Formulated magnetic nanoparticles were tested for drug release, surface morphology, antibacterial activity and FTIR. Results: It was observed from the findings that both formulations were effectively loaded with drugs. It was also found that the release of drug levofloxacin was sustained over a period of 48 hrs. The SEM results showed the semi spherical nanoparticles effectively loaded with...
Pharmaceutical Nanotechnology
Background and Objectives: The combination of nano-metals and antibacterial agents could improve the efficacy of antibiotics against pathogens. This study suggested a combination method for increasing the antibacterial and anti-biofilm activity of ampicillin and gentamicin using iron oxide nanoparticles. Method: The synthesis of lipoamino acid-coated IONs (LION14) was done by co-precipitation method. The LION14s were characterized by several techniques. The antimicrobial and biofilm inhibitory activities of nanoparticles at three-time intervals were investigated alone and in combination with ampicillin and gentamicin against Staphylococcus aureus and Escherichia coli. In-vitro cytotoxicity assay was performed to assess potential toxic effects of LION14 on mammalian cell line. Results: Detailed characterization of the LION14 confirmed the presence of about 7 nm sized magnetic nanoparticles coated with lipo-amino acid. The antimicrobial and biofilm inhibitory effects of ampicillin and gentamicin were increased in the presence of the appropriate concentration of LI-ON14s against tested microorganisms. The highest synergistic effect was observed for ampicillin against Escherichia coli. Also in the presence of antibiotics, the antibacterial and biofilm inhibitory effect of LION14 was significantly increased. The cytotoxicity results of LION14 showed the minimum cytotoxicity on the L929 cell line. Conclusion: The result showed that the combination of antibiotics with LION14 provides enhanced antimicrobial and anti-biofilm results for antibiotics along with acceptable biocompatibility. This synergistic effect could be used against biofilm forming bacteria and resistant microorganisms in the future.
Acta Biomaterialia, 2012
Biofilms on biomaterial implants are hard to eradicate with antibiotics due to the protection offered by the biofilm mode of growth, especially when caused by antibiotic-resistant strains. Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used in various biomedical applications, such as targeted drug delivery and magnetic resonance imaging. Here, we evaluate the hypothesis that SPIONs can be effective in the treatment of biomaterial-associated infection. SPIONs can be targeted to the infection site using an external magnetic field, causing deep penetration in a biofilm and possibly effectiveness against antibiotic-resistant strains. We report that carboxyl-grafted SPIONs, magnetically concentrated in a biofilm, cause an approximately 8-fold higher percentage of dead staphylococci than does gentamicin for a gentamicin-resistant strain in a developing biofilm. Moreover, magnetically concentrated carboxylgrafted SPIONs cause bacterial killing in an established biofilm. Thus magnetic targeting of SPIONs constitutes a promising alternative for the treatment of costly and recalcitrant biomaterial-associated infections by antibiotic-resistant strains.