Surface-enhanced Raman scattering biomedical applications of plasmonic colloidal particles (original) (raw)
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Surface-enhanced Raman scattering (SERS) techniques offer a number of advantages in molecular detection and analysis, particularly in terms of the multiplex detection of biomolecules. So far, many new SERS-based substrates and analytical techniques have been reported. For easy understanding, various SERS techniques are classified into the following four categories: adsorption-mediated direct detection; antibody- or ligand-mediated direct detection; binding catalyzed indirect detection; and tag-based indirect detection. Among these, recent successes of SERS tagging/encoding (nano/micro) materials and detection methods are highlighted, including our recent works. Some novel SERS-based strategies for the detection of several biological molecules are also introduced.
2017
Plasmonic nanostructures have attracted considerable interest in biomarker sensing with the goal of rapid diagnostics and personalized nanomedicine. Surface‐enhanced Raman scattering (SERS) is a versatile technique for the characterization of the plasmonic effect of the metallic nanostructures as well as a sensitive read‐out approach for biomarkers detection. In this contribution, we will give a review on the key optical properties of plasmonic nanostructures as SERS substrate for protein biomarkers detection. As a con‐ sequence, two approaches, label‐free and SERS labels will be discussed in details for pro‐ tein biomarkers sensing by using the plasmonic nanostructures as the substrate.
Plasmonics in Biology and Medicine, 2004
The intense color of noble metal nanoparticles has inspired artists and fascinated scientists for hundreds of years. These rich hues are due to the interaction of light with the nanostructure's localized surface plasmon (LSPR). Here, we describe three optical sensing modalities that are dependant on the effects of the LSPR. Specifically, we will demonstrate the use of LSPR supporting particles as analogues to fluorescent probes and labels for multiplex detection, sensing based on observation of changes in the LSPR spectrum caused by alteration of the local refractive index upon analyte binding, and the spectroscopic labeling of cells and tissues with Surface Enhanced Raman Scatting (SERS) active nanoparticles probes.
Drug Metabolism Reviews, 2014
In this review of the literature on surface-enhanced Raman scattering (SERS), we describe recent developments in SERS techniques in medical technology. The SERS technique has developed rapidly in the last few years as a result of fascinating advancements in instrumentation and the ability to interpret Raman data using high-processional, computer-aided programs. SERS has many advantages over ordinary spectroscopic analytical techniques -such as extremely high sensitivity, molecular selectivity, intense signal and great precision -that can be leveraged to address complex medical diagnostics problems. This review focuses on the SERS-active substrate, as well as major advances in cancer and bacteria detection and imaging. Finally, we present a perspective on anticipated future advancements in SERS techniques to address some of the most critical challenges in the areas of diagnostics, detection, and sensing.
Nano Research, 2018
Although nanotechnology has led to important advances in in vitro diagnostics, the development of nanosensors for in vivo detection remains very challenging. Here, we demonstrated the proof-of-principle of in vivo detection of nucleic acid targets using a promising type of surface-enhanced Raman scattering (SERS) nanosensor implanted in the skin of a large animal model (pig). The in vivo nanosensor used in this study involves the "inverse molecular sentinel" detection scheme using plasmonics-active nanostars, which have tunable absorption bands in the near infrared region of the "tissue optical window", rendering them efficient as an optical sensing platform for in vivo optical detection. Ex vivo measurements were also performed using human skin grafts to demonstrate the detection of SERS nanosensors through tissue. In this study, a new core-shell nanorattle probe with Raman reporters trapped between the core and shell was utilized as an internal standard system for self-calibration. These results illustrate the usefulness and translational potential of the SERS nanosensor for in vivo biosensing.
Surface-enhanced Raman scattering: a new optical probe in molecular biophysics and biomedicine
Theoretical Chemistry Accounts, 2010
Sensitive and detailed molecular structural information plays an increasing role in molecular biophysics and molecular medicine. Therefore, vibrational spectroscopic techniques, such as Raman scattering, which provide high structural information content are of growing interest in biophysical and biomedical research. Raman spectroscopy can be revolutionized when the inelastic scattering process takes place in the very close vicinity of metal nanostructures. Under these conditions, strongly increased Raman signals can be obtained due to resonances between optical fields and the collective oscillations of the free electrons in the metal. This effect of surface-enhanced Raman scattering (SERS) allows us to push vibrational spectroscopy to new limits in detection sensitivity, lateral resolution, and molecular structural selectivity. This opens up exciting perspectives also in molecular biospectroscopy. This article highlights three directions where SERS can offer interesting new capabilities. This includes SERS as a technique for detecting and tracking a single molecule, a SERS-based nanosensor for probing the chemical composition and the pH value in a live cell, and the effect of socalled surface-enhanced Raman optical activity, which provides information on the chiral organization of molecules on surfaces.
Surface-enhanced Raman scattering (SERS) has demonstrated single-molecule sensitivity and is becoming intensively investigated due to its significant potential in chemical and biomedical applications. SERS sensing is highly dependent on the substrate, where excitation of the localized surface plasmons (LSPs) enhances the Raman scattering signals of proximate analyte molecules. This paper reviews research progress of SERS substrates based on both plasmonic materials and nano-photonic structures. We first discuss basic plasmonic materials, such as metallic nanoparticles and nano-rods prepared by conventional bottom-up chemical synthesis processes. Then, we review rationally-designed plasmonic nano-structures created by top-down approaches or fine-controlled synthesis with high-density hot-spots to provide large SERS enhancement factors (EFs). Finally, we discuss the research progress of hybrid SERS substrates through the integration of plasmonic nano-structures with other nano-photonic devices, such as photonic crystals, bio-enabled nanomaterials, guided-wave systems, micro-fluidics and graphene.
SERS is a thirty years old physical phenomenon that has become one of the most exciting analytical techniques with a wide range of applications in physics, chemistry and biology, and the corresponding applied sciences. This ultrasensitive analytical tool covers the complete scale of sensitive analysis and diagnostics down to the limit of single molecule detection (SMD). There are now thousands of SERS publications in a wide range of scientifi c journals (for instance, SERS papers can be found in all the journals published by the ACS). The applications are relevant to trace analysis, environmental monitoring, nanobioscience among many others. The fi eld is intrinsically connected with the optical properties of nanostructures and SERS techniques are also used for nanostructure characterization, since it can be discussed in terms of surface plasmon resonance of nanoparticles, nanoshells or voids, leading to nanophotonics, plasmonics and single-molecule detection. Therefore, nowadays, no attempt or claim can be made to review the fi eld exhaustively in its entirety nor to cover all applications. Some of the above invited speakers contributed to the present issue. The speakers and the audience were able to exchange their views and newly acquired results in lively and sometimes heated discussions. The poster session was also quite active and successful. A total of 49 posters were displayed during the symposium. The following presentations received the Poster Awards. H. K. Park et al. "Silver-Particle-Based Surface-Enhanced Raman Scattering Spectroscopy for Biomolecular Sensing and Recognition" M. Meyer et al. "Can the Orientation of Molecules be Measured with SERS?" A. Maio et al. "SERRS measurement and Structural Characterization of J-and H-Aggregates in Langmuir-Blodgett (LB) Films Containing Merocyanine Dye"