Protective Efficacy and Immunogenicity of an Adenoviral Vector Vaccine Encoding the Codon-Optimized F Protein of Respiratory Syncytial Virus (original) (raw)

1 2 Adenoviral vectors (AdV) have received considerable attention for vaccine development 3 because of their high immunogenicity and efficacy. In previous studies it was shown that 4 DNA immunization of mice with codon optimized expression plasmids encoding the fusion 5 protein of the respiratory syncytial virus (RSV-F) resulted in enhanced protection against 6 RSV challenge compared to plasmids carrying the wild type cDNA sequence of RSV-F. Now 7 we constructed AdV carrying the codon optimized full-length (AdV-F) or the soluble form 8 (AdV-Fsol) of the RSV-F gene. BALB/c mice were immunized twice with AdV-F or AdV-9