Comparative Antioxidant Potential of Different Extracts of Celastrus paniculatus Willd. Seed (original) (raw)
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In vitro and in vivo antioxidant activities of the aqueous extract of Celosia argentea leaves
Indian Journal of Pharmacology, 2011
Strychnos henningsii Gilg is used traditionally for the treatment of various ailments in southern Africa traditional medicine. The antioxidant and free radical scavenging activity of aqueous extract of this plant was investigated both in -vivo and -vitro using spectroscopic method against 1,1-diphenyl-2picrylhydrazyl (DPPH), superoxide anions, hydrogen peroxide (H 2 O 2 ), nitric oxide (NO), 2,2'-azinobis [3-ethylbenzothiazoline-6-sulfonic acid] diammonium salt (ABTS) and the ferric reducing agent. Total phenols, flavonoid, flavonol and proanthocyanidin were also determined to assess their effects on the antioxidant activity of this plant. Free radical scavenging activity of the plant extract against H 2 O 2 , ABTS and NO was concentration dependent with IC 50 value of 0.023, 0.089 and 0.49 mg/ml respectively. However, S. henningsii exhibited lower inhibitory activity against DPPH with IC 50 value of 0.739 mg/ml. The reducing power of the extract was found to be concentration dependent. The administration of the aqueous extract at 250, 500 and 1000 mg/kg body weight to male Wistar rats significantly increased the percentage inhibition of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). Whereas, lipid peroxidation level in hepatotoxic rats decreased significantly at the dose of 500 and 1000 mg/kg body weight at the end of 7 days. The extract yielded high phenol content (48 mg/g tannic acid equivalent) followed by proanthocyanidin (8.7 mg/g catechin equivalent) flavonol (5.5 mg/g quercetin equivalent) and flavonoids (4.8 mg/g quercetin equivalent) respectively. A positive linear correlation was observed between these polyphenols and the free radical scavenging activities.
Medicines, 2019
Background: In this study, column chromatography was applied to separate active fractions from the ethyl acetate extract of Celastrus hindsii, a medicinal plant widely used in Southern China, Northern Vietnam, Myanmar, and Malaysia. Methods: Fourteen fractions from different dilutions of chloroform and methanol were separated by column chromatography and examined for biological activities. Results: It was found that a dilution of 50–70% methanol in chloroform yielded the highest total phenolics, flavonoids, and antioxidant activities (1,1-dipheny1-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging activity, and β-carotene bleaching method measured by lipid peroxidation inhibition). In addition, by gas chromatography-mass spectrometry (GC-MS) and electrospray ionization-mass spectrometry (ESI-MS) analyses, fifteen principal compounds from bioactive fractions belonging to fatty acids, amides, flavonoids, sterols, t...
Antioxidant properties of leaves and seeds hydromethanolic extracts from Celtis australis
The present study investigated the antioxidant activity of hydromethanolic extracts of leaves and seeds from Celtis Australis. The antioxidant property of both plant extracts was assessed by in vitro measurement of DPPH (1,1'-diphenyl-2picrylhydrazil) radical scavenging activity and conjugated Dienes (CD), TBARS (thiobarbituric acid reactive substances) and malondialdehyde (MDA) inhibition during linoleic acid peroxidation. At the acid concentrations (2, 10 and 20mg/ml), the radical scavenging activities were 46, 59 and 88% and 44, 57 and 86% respectively for leaves and seed extracts. In the same conditions the rates of conjugated Dienes inhibition were 64, 85 and 92% and 58, 82 and 91% respectively with leaves and seed extracts. In the same way, the rates of TBARS inhibition were 57, 78 and 87% and 55, 78 and 81% respectively with leaves and seed extracts. Both extracts induced a diminution of MDA generation. All the obtained results were significant (P < 0.05) and concentration-dependent. Similar results were exhibited by standard antioxidant BHT, used in 15, 20 and 25µg/ml. The plant extracts yielded higher polyphenolics content followed by phenols, flavaonoids and condensed tannins. A positive linear correlation was established between these compounds and the antioxidant activity of plant extracts. The results obtained from this study indicate that C. Australis is a potent source of antioxidants and thus could prevent many radical related diseases.
Journal of Applied Biology & Biotechnology, 2018
The study was performed to check the chemical, antioxidant, antimicrobial, and protein binding qualities of the hydroethanolic extract of Celastrus paniculatus (CP). Hydroethanolic extract of CP exhibited low antibacterial and antifungal activities. Excellent antioxidant activities (60-80%) were noticed for various antioxidant assays, namely, 2,2-diphenyl-1-picrylhydrazyl, metal chelating, ferric reducing ability of plasma, superoxide radical, and nitric oxide. Total phenolic content (TPC) and total flavonoid content (TFC) of the extract were 1.38 ± 0.15 mg/g (expressed in equivalents to gallic acid) and 8.91 ± 0.21 mg (expressed in equivalents quercetin), respectively. Moreover, at P ≤ 0.05, the significant linear correlation was noticed between the TFC/TPC and antioxidant activities favored which highlights the medicinal property of CP. Protein binding assay was performed, and an observed binding constant (1.11 ± 0.92 × 10 −5 /µM) was almost close to control (aspirin). Anti-inflammatory assay revealed the potent anti-inflammatory activity of the hydroalcoholic extract of CP. These significant findings of CP may provide compelling scientific evidence of its therapeutic potential.
The present study was carried out to compare the secondary metabolites, in vitro and in vivo antioxidant activities as well as the safety of ethyl acetate and methanolic extracts of Celosia argentea leaves in cadmium-induced oxidative stress in rats. The secondary metabolite screening was done by standard methods while the in vitro antioxidant activity of the extract was evaluated using ammonium thiocyanate, reducing power and diphenyl picryl hydrazyl (DPPH) radical scavenging models. In the in-vivo antioxidant and toxicological studies, thirty rats (Rattus novergicus) weighing 137.05 ± 5.84g were completely randomized into six groups (A-F) of five animals each. Animals in group A received orally 0.5ml of distilled water for 7 days while those in groups B, C, D, E and F received same volume corresponding to 8 mg/kg body weight (bw) of cadmium, in addition to simultaneous administration of distilled water, 100 mg/kg b.w of ascorbic acid, 100, 200 and 400 mg/kg b.w. of the extract respectively. Biochemical indices of in vivo antioxidant activities and toxicity were evaluated in the animals after the treatment period. The ethyl acetate extract of C. argentea contained saponins (1.67%), tannins (0.65%), cardenolide and dienolides (1.20%) and phenolics (0.42%) whereas the methanolic extract contained saponins (3.20%), tannins (0.65%), cardenolide and dienolides (0.006%) and phenolics (5.72%). Reducing sugar, steroids, and glycosides were only detected in the ethylacetate extract. The ethyl acetate extract and ascorbic acid, at 50 mg/ml, inhibited linoleic acid oxidation by 51.00 and 24.2% respectively whereas the methanolic extract produced 51.01% inhibition. Ethylacetate extract at 10, 50 and 100 mg/ml produced reducing power of 0.116, 0.092 and 0.127 nm whereas the methanolic extract produced 0.131, 0.185 and 0.183nm when compared with ascorbic acid that gave 0.092, 0.089 and 0.107 nm. The 100 μg/ml of both the ethyl acetate and methanolic extracts scavenged 82% and 30% respectively of the DPPH radical as against 65% in ascorbic acid. Both the extracts attenuated the cadmium chloride treatment related reduction in the activities of superoxide dismutase, catalase, alkaline phosphatase, gamma glutamyl transferase, alanine and aspartate transaminase as well as the levels of uric acid, albumin, total and conjugated bilirubin, total protein and the Cd elevated levels of malondialdehyde in the serum and tissues of the animals in a manner similar to that of the ascorbic acid treated animals and the non-Cd treated animals administered distilled water; with the ethyl acetate producing a better result. The totality of the results conferred antioxidant activity on the ethyl acetate extract and methanolic extract by the phenolic components of the extracts via induction of the antioxidant enzymes and scavenging of free radical. The extracts also reversed cadmium induced changes in the biomarkers of liver damage.
Journal of Ayurveda and Integrated Medical Sciences (JAIMS), 2020
More than 35,000 plant species are being used in various human cultures around the world for medicinal purposes. Medicated oil plays an important role for prevention and cure of diseases in Ayurveda. Celastrus paniculatus seed oil is such wonderful medicinally useful oil. It is commonly known as Black-Oil tree, Intellect tree, Climbing-staff plant and Jyotishmati and “Tree of life” in Ayurveda. It possesses Anti-depressant, Anti-Parkinson, Anti-Alzheimer’s, Neuroprotective, IQ improving activity. In tribal district (Jashpur) of Chhattisgarh, Indian people uses traditional method to extract C. paniculatus oil. It is commonly used by the tribes for the treatment of various diseases like headache, muscular spasm and local inflammations. The present study aim is documentation of traditional method of oil extraction used by ‘Uraanv’ tribe. This paper also dealt with the Physicochemical and qualitative phytochemical evaluation of C. paniculatus seed oil. Oil yield by traditional method wa...
Heliyon, 2020
This study investigated the phytochemical constituents and antioxidant properties of crude extracts of C. argentea at different maturity stages and seasons. Total phenols, flavonoids, and proanthocyanidin content from water, acetone and methanol extracts were evaluated spectrophotometrically. The antioxidant activities were measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2 0-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), Ferric reducing antioxidant power (FRAP) and Total antioxidant capacity (TAC) models. Results showed that the flowering stages in all the solvent extracts gave the highest polyphenolic content with the acetone extract significantly higher than the methanol and aqueous extracts (P < 0.05). The highest value for total polyhenolic content 80.75 AE 4.21 for the first trial and 89.69 AE 2.13 μg/mL in the second trial; while the flavonoids was 874.76 AE 7.87 and 946.19 AE 7.87 μg/mL in the first and second trials respectively; and proanthocyanidin content was 170.00 AE 0 and 100.90 AE 1.29 μg/mL. Overall, the aqueous extracts had the lowest content of all the phytochemicals. The antioxidant activities ranged from low to high at different growth stages of the plant. While low to no activity was observed in the aqueous extracts in all the assays, the methanol extracts of the flowering stages showeds the best activity in the first and second trials with IC 50 values of 104.10 AE 8.59 and 120.02 AE 13.37 μg/mL respectively in ABTS. Similar trend was obtained in the DPPH assay with the highest activity in the methanol flowering extract with IC 50 of 52.36 AE 0.76 μg/mL (first trial) and 49.36 AE 0.29 μg/mL (second trial). The FRAP and TAC also had the highest activity in the flowering stages in all solvents, but with the acetone extracts having the overall inhibition on both radicals. This study revealed that Celosia argentea phytoconsituents and antioxidant potential can be influenced by physiological and developmental stages of the plant.
Heliyon, 2020
This study was undertaken to investigate the nutritional value, chemical characterization and in-vitro antioxidant activity of Celosia cristata Linn. inflorescences, a culturally significant plant of Kashmir valley, India. The results revealed that the flower contained variety of vitamins (A, B-complex, C and E) with Vitamin E (tocopherol) showing the highest concentration. Among minerals, potassium was found to be present in significant amounts, the amino acid and fatty acid profile of the flower was also found to be satisfactory. The antioxidant activity of flower extract was evaluated by various in-vitro analytical methods: DPPH free radical scavenging activity, lipid peroxidation, reducing power, and metal chelating ability. Therefore, the present research brings into focus, the nutritional and antioxidant potential of C. cristata flower and its extract.
Investigation of Antioxidant Activity and Phytochemical Compositions of Celtis Tournefortii
Free Radicals and Antioxidants, 2017
Context: Phytochemicals are non-nutritive plant chemicals that have protective or disease preventive properties. Because they have antioxidant property and protect our cells against oxidative damage. Aim: This work aimed to determination of phytochemical composition which total phenol content(TPC), total flavonoid content(TFC), total flavones content, alkaloid, acidity, pH, total carotenoid content, amount of carotene, β-carotene concentration, anthocyanin, amount of saponin and tannin, ascorbic acid levels, mineral profile and antioxidant activity of fresh Celtis tournefortii due to the importance of secondary metabolites. Material and method: Celtis tournefortii was used for this study Phytochemical compositions were determined by spectrometric methods excluding phenolic acid and mineral profile. Antioxidant activity were determined by different antioxidant method. Consequently: Celtis tournefortii may be an antioxidant source.