Effect of training on the response of plasma vascular endothelial growth factor to exercise in patients with peripheral arterial disease (original) (raw)
Related papers
Circulating plasma VEGF response to exercise in sedentary and endurance-trained men
Journal of Applied Physiology, 2004
The skeletal muscle capillary supply is an important determinant of maximum exercise capacity, and it is well known that endurance exercise training increases the muscle capillary supply. The muscle capillary supply and exercise-induced angiogenesis are regulated in part by vascular endothelial growth factor (VEGF). VEGF is produced by skeletal muscle cells and can be secreted into the circulation. We investigated whether there are differences in circulating plasma VEGF between sedentary individuals (Sed) and well-trained endurance athletes (ET) at rest or in response to acute exercise. Eight ET men (maximal oxygen consumption: 63.8 ± 2.3 ml·kg-1·min-1; maximum power output: 409.4 ± 13.3 W) and eight Sed men (maximal oxygen consumption: 36.3 ± 2.1 ml·kg-1·min-1; maximum power output: 234.4 ± 13.3 W) exercised for 1 h at 50% of maximum power output. Antecubital vein plasma was collected at rest and at 0, 2, and 4 h postexercise. Plasma VEGF was measured by ELISA analysis. Acute exerc...
Journal of Applied Physiology, 2005
VEGF-A contributes to muscle tissue angiogenesis following aerobic exercise training. The temporal response of the VEGF-A isoforms and their target receptors has not been comprehensively profiled in human skeletal muscle. We combined submaximal exercise with and without reduced leg blood flow to establish whether ischemia-induced metabolic stress was an important physiological stimuli responsible for regulating the VEGF-A system in humans. Nine healthy men performed two 45-min bouts of one-leg knee-extension exercise, with and without blood flow restriction. Muscle biopsies were obtained at rest and 2 and 6 h after exercise. Expression (mRNA) of the VEGF-A splice variants and related receptors [VEGF receptor (VEGFR)-1, VEGFR-2, and neuropilin-1] was determined by using qPCR. VEGF-Atotal expression increased more robustly after exercise with reduced blood flow, and initially this principally reflected an increase in VEGF-A165. Six hours after exercise, there was a relatively greater ...
2011
Selective breeding for high voluntary wheel running in untrained mice has resulted in a ‘mini muscle’ (MM) phenotype, which has increased skeletal muscle capillarity compared with muscles from non-selected control lines. Vascular endothelial growth factor (VEGF) and thrombospondin-1 (TSP-1) are essential mediators of skeletal muscle angiogenesis; thus, we hypothesized that untrained MM mice with elevated muscle capillarity would have higher basal VEGF expression and lower basal TSP-1 expression, and potentially an exaggerated VEGF response to acute exercise.We examined skeletalmuscle morphology and skeletalmuscle protein expression of VEGF and TSP-1 in male mice from two (untrained) mouse lines selectively bred for high exercise capacity (MM and Non-MM), as well as one non-selected control mouse line (normal aerobic capacity). In the MM mice, gastrocnemius (GA) and plantaris (PLT) muscle capillarity (i.e. capillary-to-fibre ratio and capillary density) were greater compared with control mice (P<0.05). In Non-MM mice, only muscle capillarity in PLT was greater than in controlmice (P <0.001). The soleus (SOL) showed no statistical differences inmuscle capillarity among groups. In the GA,MMmice had 58% greater basal VEGF (P <0.05), with no statistical difference in basal TSP-1 when compared with control mice. In the PLT, MM mice had a 79% increase in basal VEGF (P <0.05) and a 39% lower basal TSP-1 (P <0.05) compared with the control animals. Non-MMmice showed no difference in basal VEGF in either the GA or the PLT compared with control mice. In contrast, basal TSP-1 was elevated in the PLT, but not in the GA, of Non-MM mice compared with control mice. Neither VEGF nor TSP-1 was significantly different in SOL muscle among the three mouse lines. In response to acute exercise, MM mice displayed a 41 and 28% increase (P <0.05) in VEGF in the GA and PLT, respectively, whereas neither control nor Non-MM mice showed a significant VEGF response to acute exercise. In contrast, TSP-1 levels were decreased by 90% in GA (P <0.05) but increased by 50% in PLT (P <0.05) in response to acute exercise inMMmice. The SOL showed no response to exercise for either VEGF or TSP-1 for any of the mouse lines. These data, with the exception of the Non-MM plantaris muscle, suggest that elevated capillarity is associated with altered balance between positive and negative angiogenic regulators (i.e. VEGF versus TSP-1, respectively). Based on the greater capillarity and significant VEGF response to exercise in MM mice, these data suggest that VEGF expression may, at least in part, be genetically determined.
Revista argentina de cardiología
background: The aim of this study was to assess the effect of acute and programmed physical exercise on plasma VEGF levels in chronic stable coronary artery disease patients. Following baseline evaluation, 21 patients <75 years underwent an exercise stress myocardial perfusion scan (acute), and were then randomly assigned to perform programmed rehabilitation exercise or continue with their normal therapy. VEGF assessed by SPECT imaging significantly decreased after stress ergometry (from 49.59±6.06 to 31.83±5.62 pg/ml; p=0.021). At one month, it increased (70.90±14.44 pg/ml) though not significantly with respect to baseline values (p=0.1) and significantly with respect to immediate post exercise values (p<0.01). No significant changes were observed in VEGF at 3 months or when results were compared according to the presence of ischemia or programmed exercise. Acute exercise induced a significant reduction in VEGF values, without differences between programmed exercise and the c...
Pakistan BioMedical Journal
Endostatin and Vascular Endothelial Growth Factor (VEGF) are important markers driving the angiogenic switch. It is clear that short periods of moderate to high intensity exercise significantly increase the concentration of endostatin and VEGF in plasma. Objective: To investigate concentration of circulatory endostatin in plasma and impact of different intensities of exercise encompassing from low to maximum on distribution of endostatin and VEGF concentrations in plasma. Methods: Eight healthy male volunteers were recruited through advertisements and personal contacts, after assessing their fitness through two pre-participation health screening questionnaires, PAR-Q and ACSM Health Fitness Facility pre-participation health screening questionnaire for performing maximal exercise to volitional exhaustion. All the volunteers attend the lab on 2 consecutive days. The blood was centrifuged at 1000 RPM for 15 minutes for endostatin and VEGF and at 3000 RPM for 15 minutes for lipid profil...
Brazilian Journal of Medical and Biological Research, 2008
Exercise-induced vessel changes modulate arterial pressure (AP) in male spontaneously hypertensive rats (SHR). Vascular endothelial growth factor (VEGF) is important for angiogenesis of skeletal muscle. The present study evaluated the time course of VEGF and angiogenesis after short-and long-term exercise training of female SHR and Wistar Kyoto (WKY) rats, 8-9 weeks (200-250 g). Rats were allocated to daily training or remained sedentary for 3 days (N = 23) or 13 weeks (N = 23). After training, the carotid artery was catheterized for AP measurements. Locomotor (tibialis anterior and gracilis) and non-locomotor skeletal muscles (temporalis) were harvested and prepared for histologic and protein expression analyses. Training increased treadmill performance by all groups (SHR = 28%, WKY = 64%, 3 days) and (SHR = 141%, WKY = 122%, 13 weeks). SHR had higher values of AP than WKY (174 ± 4 vs 111 ± 2 mmHg) that were not altered by training. Three days of running increased VEGF expression (SHR = 28%, WKY = 36%) simultaneously with an increase in capillary-to-fiber ratio in gracilis muscle (SHR = 19%, WKY = 15%). In contrast, 13 weeks of training increased gracilis capillary-to-fiber ratio (SHR = 18%, WKY = 19%), without simultaneous changes in VEGF expression. Training did not change VEGF expression and capillarity of temporalis muscle. We conclude that training stimulates time-and tissue-dependent VEGF protein expression, independent of pressure levels. VEGF triggers angiogenesis in locomotor skeletal muscle shortly after the exercise starts, but is not involved in the maintenance of capillarity after long-term exercise in female rats.
3 The Effect of Two Types of Physical Activity on Serum VEGF-A Response in Non-Athletic Men
2014
Corresponding Author : Tel : 09185403074, Email : m.baranchi@modares.ac.ir Sport Biosciences, Vol.6, No.1, Spring 2014 The aim of this study was to describe and examine the relationships in physical fitness, anthropometric and body composition profile male adolescent players of Iran national volleyball team. The sample of this study consisted of 40 players (Age: 17.9±0.3) present in the preparation camp of national adolescent volleyball team. Body composition factors (weight, BMI, WHR, body fat percentage, muscle mass) and anthropometric indexes (girths: waist, hip, abdomen, forearm, arm, thigh, calf; breadths: wrist, humerus, femur and lengths: stature, standing reach height, arm span, midstylion-dactylion, radiale-stylion, tibiale mediale, sphyrion tibiale) were measured. Also, physiological and physical fitness variables: heart rate, blood pressure, basal metabolic rate (BMR), long jump, bar fix, sit up, 20-m sprint, 4×9 m agility, spike jump, block jump, anaerobic power (15 s ...