Successful birth following transfer of frozen–thawed embryos produced from in-vitro matured oocytes (original) (raw)
Related papers
Journal of Reproduction and Development, 2007
The present report describes the birth of a healthy infant after cryopreservation of embryos produced from in vitro-matured oocytes retrieved from a woman at risk of developing ovarian hyperstimulation syndrome (OHSS) during conventional in vitro fertilization (IVF) cycles. A conventional long protocol including gonadotropin-releasing hormone agonist (GnRHa) and gonadotropins induced a risk of OHSS. Oocyte retrieval was performed on day 11 of the cycle, and 27 immature oocytes were obtained. Following incubation for 24 h in maturation medium, 74.1% (20/27) of the oocytes were at the metaphase II stage. Fourteen oocytes (14/20, 70.0%) were fertilized after intracytoplasmic sperm injection (ICSI) with her husband's spermatozoa and cultured for 3 days. On day 4 following oocyte retrieval, three embryos at the 8-16 cell stage were transferred into the woman's uterus, and five spare embryos were frozen. Since the fresh embryo transfer failed to result in pregnancy, three post-thaw embryos were transferred into the woman three months later. Transfer of the frozen embryos resulted in pregnancy with delivery of a healthy infant girl.
Frozen embryo transfers: implications of clinical and embryological factors on the pregnancy outcome
Human Reproduction, 2006
BACKGROUND: Frozen embryo transfers are characterized by impaired pregnancy outcome and increased incidence of pregnancy loss as compared with fresh IVF/ICSI embryo transfers. In this study, we performed a retrospective analysis of clinical and embryological factors that potentially influence the outcome of frozen embryo transfer. METHODS: We reviewed the outcome of 1242 frozen embryo transfers with respect to the age of the woman, the method of fertilization, embryo quality before and after freezing and the number of embryos transferred. RESULTS AND CONCLUSIONS: The pregnancy (positive hCG) and clinical pregnancy rates were 25.8 and 21.1%, respectively. A total of 107 (33.3%) of the 321 pregnancies identified by a positive hCG test miscarried either before (18.4%) or after (15%) the clinical recognition of gestational sac(s). The delivery rate for the frozen embryo transfers analysed was 17.2%. Our data revealed that the delivery rate after frozen embryo transfer was dependent on both the woman's age and the quality of embryos transferred, at the same time being unaffected by IVF/ICSI treatment. In addition, the increased woman's age at IVF/ICSI treatment was identified as the only parameter elevating the biochemical pregnancy rate, whereas the clinical abortion rate was found to be unrelated to the clinical or embryological parameters studied.
Clinical factors associated with live birth after single embryo transfer
Fertility and Sterility, 2012
Objective: To identify patient, cycle, and retrieval characteristics associated with embryo implantation and live birth in patients undergoing single embryo transfer (SET). Design: Analysis of prospectively collected IVF database. Setting: Academic IVF program. Patient(s): All patient cycles meeting criteria for SET between June 2004 and September 2010. Intervention(s): None. Main Outcome Measure(s): Clinical pregnancy and live birth. Result(s): Single embryo transfer was performed in 438 cycles, resulting in a clinical pregnancy rate of 76.2% and a live birth rate of 66.8% per transfer. Clinical pregnancy was associated with younger female age, R58% mature (metaphase II) oocytes at the time of retrieval, and increasing blastocyst expansion. Ongoing pregnancy was associated with younger female age and more advanced blastocysts. A diagnosis of uterine factor was negatively associated with live birth. Conclusion(s): Even in a favorable prognosis population, younger female age is associated with clinical pregnancy and live birth. Although all patients underwent blastocyst transfer, expanded and hatching blastocysts were strongly associated with pregnancy and live birth. A diagnosis of uterine factor was the only infertility diagnosis found to affect live birth after SET. Obesity did not negatively affect SET outcome. These findings may assist physicians in determining the best candidates for SET. (Fertil Steril Ò 2012;98:1152-6. Ó2012 by American Society for Reproductive Medicine.
Fertility and Sterility, 2008
Objective: To report a case of successful delivery of a healthy baby after intracytoplasmic sperm injection (ICSI) in a patient with no mature oocytes at the time of oocyte retrieval. Design: Case report. Setting: Department of Reproductive Medicine. Patient: After a controlled ovarian hyperstimulation cycle, a total of seven immature oocytes were collected. Intervention(s): Medical management. Main outcome measure(s): The timing of polar body extrusion was checked every 2 hours, and ICSI was performed as soon as the first polar body was extruded. Result(s): Following incubation in culture medium, five oocytes reached the metaphase II stage within 8-8.5 hours. Three oocytes were fertilized after ICSI, and two of three cleaved embryos were transferred on day 3. The embryo transfer was followed by a single pregnancy and the delivery of a healthy baby. Conclusion: This case report demonstrates that embryos obtained from in vitro matured oocytes retain the developmental competence for full-term. It illustrates the importance of regularly monitoring the polar body extrusion when all collected oocytes are immature. (Fertil Steril Ò 2008;89:991.e1-4.
Fertility and Sterility, 2010
To assess the efficacy of elective single embryo transfer (e-SET) compared to a double embryo transfer (DET) in a selected population including obstetrical and neonatal outcome of fresh and frozen-thawed cycles. Prospective nonrandomized study. Department of reproductive medicine. Elective single embryo transfer was proposed to women < 36 years with adequate ovarian function, in their first or second IVF or intracytoplasmic sperm injection (ICSI) attempt with ejaculated sperm, with at least 4 mature oocytes and 2 fertilized top quality embryos. Patients who refused e-SET had two top embryos transferred (DET group). Medical management and IVF-ICSI. Cumulative delivery rate, twin delivery rate, obstetrical and neonatal outcome. According to patients' decision, 53 women had an e-SET and 98 a DET. The cumulative delivery rate per patient was 54.7% in the e-SET group and 49.0% in the DET group (P>0.05). Twin delivery rate was significantly different between the two groups (3.5% versus 37.5% respectively, P<0.05). Neonatal outcome in twins resulting from IVF-ICSI was found to be poorer than in singletons, considering the mean gestational age, mode of delivery, birthweight, and risk of neonatal intensive care unit admission for the infants. In a selected population, the elective transfer of one embryo with high implantation potential helped to avoid twin pregnancies without decreasing delivery rate.
Ovarian pregnancy after in-vitro fertilization with embryo transfer: A case report
Case Report, 2024
Background: Ovarian pregnancy, a rare but life-threatening complication, has seen an increase in incidence with the development of assisted reproduction technology (ART). The exact mechanism of ovarian pregnancy after IVF remains unclear; however, factors such as reverse migration of embryos and in-vivo fertilization of anovulated oocytes have been proposed. Case Presentation: We present a case of a 42-year-old woman with poor ovarian reserve who underwent IVF-ET using donor follicles. Despite adherence to instructions against coitus during and after the IVF cycle, a transvaginal ultrasound revealed a right ovarian pregnancy, which ruptured during ultrasound examination. The patient underwent exploratory laparotomy, and had a satisfactory recovery. Conclusion: This case emphasizes the importance of considering ovarian pregnancy in the differential diagnosis of ectopic gestations post-IVF as early recognition and intervention are paramount for optimal patient outcomes. Surgical treatment with wedge resection of the ovary alongside the gestation or product of conception has been considered gold standard of care. Keywords: Ovarian pregnancy; Ectopic pregnancy; In vitro fertilization; Embryo transfer; Wedge resection; Oophorectomy
Obstetric outcome after in vitro fertilization with single or double embryo transfer
Human Reproduction, 2011
background: IVF children, including singletons, are known to have a poorer obstetric outcome than children born after spontaneous conception. With a broad introduction of single embryo transfer (SET), this scenario might change. This study compares the obstetric outcome after IVF with SET, elective SET (eSET), non elective SET (non-eSET) and double embryo transfer (DET) with outcomes in the general population. methods: All IVF children born in Sweden after IVF treatment during the years 2002-2006 (n ¼ 13 544 children) were included and compared with all non-IVF children born during the same time period (n ¼ 587 009 children). Data were collected from all 16 Swedish IVF clinics and cross-linked with the Swedish Medical Birth Registry. Main outcomes were preterm birth (,28, ,32 and ,37 w), verylow-birthweight (VLBW) and low-birthweight (LBW). Adjusted odds ratios were calculated.
Middle East Fertility Society Journal
Background Polycystic ovarian syndrome (PCOS) cases undergoing in vitro fertilisation (IVF) are widely at risk of ovarian hyperstimulation; therefore, elective freezing of all embryos to be transferred in a later cycle is preferred. We aimed to compare the pregnancy outcome between the fresh and the frozen embryo transfer (ET) in PCOS cases undergoing IVF with antagonist ovarian induction using human chorionic gonadotropin (HCG) as a trigger. Methods In this prospective randomised study, 110 infertile PCOS women underwent fresh ET (group A) or frozen ET (group B) with GnRH-antagonist protocol. The primary outcome was the chemical and clinical pregnancy rates. The secondary outcomes were the ongoing pregnancy rate, ovarian hyperstimulation syndrome (OHSS) rate, pregnancy loss rate, ectopic pregnancy rate, and congenital anomalies rate. Results There was no significant difference between both groups regarding chemical pregnancy rate (44.23% vs 47.27%, P = 0.752), clinical pregnancy ra...