UJI AKTIVITAS ANTIBAKTERI EKSTRAK KASAR ETANOL, KLOROFORM DAN N-HEKSANA ALGA COKLAT Sargassum vulgare ASAL PANTAI KAPONG PAMEKASAN TERHADAP BAKTERI Staphilococcus aureus dan Eschericia coli (original) (raw)

The antibacterial activity of ethanol, chloroform and n-hexane crude extract of brown algae S. vulgare from Kapong beach Pamekasan has been conducted against S. aureus and E. coli. The purpose of this research was to know the crude solvent extract showing antibacterial activities against S. aureus and E. coli and to identifity active compound groups content. The extraction of brown algae was performed by maseration ethanol, chloroform and n-hexane solvent. The crude extract were assayed an actibacterial activity by disk diffusion methode. The extract showing the best antibacterial activities were tested phytochemicals assay and TLC to identifity active compound groups. This result showed that chloroform crude extract of brown algae inhibited S. aureus in concentration of 1 % and E. coli in concentration of 5%. Phytochemical assay showed that chloroform crude extract was containing flavonoids and steroid groups. For the separation using TLC chloroform-methanol (99:1) yielded 9 spots with Rf 0,017−0,46 while chloroform-methanol (9:1) was generated 7 spots with Rf 0,17−0,91. Keywords: Brown algae S. vulgare, antibacterial test, phytochemical test, TLC