Middle-aged mice with enrichment-resistant stereotypic behaviour show reduced motivation for enrichment (original) (raw)

2010, Animal Behaviour

For captive animals, living in barren conditions leads to stereotypic behaviour that is hard to alleviate using environmental enrichment. This resistance to enrichment is often explained via mechanisms that decouple abnormal behaviour from current welfare, such as ‘establishment’: a hypothetical process whereby repetition increases behaviour’s predictability and resistance to change. If such hypotheses are correct, then animals with enrichment-resistant stereotypic behaviour should still find enrichments rewarding. Alternatively, this behaviour could reflect a failure to improve welfare: plausible because age and chronic stress increase neophobia and anhedonia. If this hypothesis is correct, animals with enrichment-resistant stereotypic behaviour should value enrichments less than conspecifics. We tested these hypotheses using C57BL/6 mice, Mus musculus, aged 10–11 and 6–7 months, raised in barren laboratory cages. We observed their behaviour in both these and large enriched cages. Enrichment was more effective on the younger animals. However, contrary to ideas about establishment, the spontaneous predictability of stereotypic behaviour did not increase with age; nor was enrichment less effective on more predictable or time-consuming forms. We assessed the reward value of enriched cages by allowing access via progressively weighted doors (maximum weight pushed corresponding to peak motivation). In older mice, those individuals whose stereotypic behaviour was least reduced by enrichment were also the least motivated to gain access to enrichment. This suggests that the welfare of middle-aged-animals, as well as their stereotypic behaviour, is harder to improve using environmental enrichment.

Effects of temporary access to environmental enrichment on measures of laboratory mouse welfare

Scientific Reports, 2024

Laboratory mice are typically housed in “shoebox” cages with limited opportunities to engage in natural behaviour. Temporary access to environments with increased space and complexity (playpens) may improve mouse welfare. Previous work by our group has shown that mice are motivated to access and use these environments, but it is unknown how other aspects of welfare are impacted. Female C57BL/6J, BALB/cJ, and DBA/2J mice (n = 21; 7 mice per strain) were housed in mixed-strain trios and given temporary access to a large playpen with their cage mates three times per week. Control mice (n = 21; 7 mice per strain) remained in their home cages. Home cage behaviour (development of stereotypic behaviour over time, aggression following cage-changing) and anxiety tests were used to assess how playpen access impacted welfare. Contrary to our predictions, we found increased time spent performing stereotypies in playpen mice; this difference may be related to negative emotional states, increased motivation to escape the home cage, or active coping strategies. Playpen access resulted in strain-dependent improvements in aggression and some measures of anxiety. Aggression was lower for C57BL/6J mice in the playpen treatment following cage changing than it was for C57BL/6J control mice, while playpen mice, and particularly the C57BL/6J strain, spent more time in the center of the open field test and produced fewer fecal boli during anxiety testing, supporting other research showing that strain differences play an important role in behaviour and stress resiliency.

Stereotypic behaviour in standard non-enriched cages is an alternative to depression-like responses in C57BL/6 mice

Behavioural brain research, 2016

Depressive-like forms of waking inactivity have been recently observed in laboratory primates and horses. We tested the hypotheses that being awake but motionless within the home-cage is a depression-like symptom in mice, and that in impoverished housing, it represents an alternative response to stereotypic behaviour. We raised C57BL/6 ('C57') and DBA/2 ('DBA') females to adulthood in non-enriched (n=62 mice) or enriched (n=60 mice) cages, observing home-cage behaviour during the active (dark) phases. We predicted that being still but awake would be reduced by environmental enrichment; more pronounced in C57s, as the strain most prone to learned helplessness; negatively related to stereotypic behaviour; and positively related to immobility in Forced Swim Tests (FST). Compared to enriched mice, non-enriched subjects did spend more time spent being inactive but awake, especially if they displayed relatively little stereotypic behaviour. C57 mice also spent more time aw...

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