Antimicrobial Effect of Different Root Canal Medicaments on Enterococcus Faecalis : in vitro Comparative Study (original) (raw)
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Effectiveness of 2% chlorhexidine on Enterococcus Faecalis in Root Canal Treatment
Root canal treatment plays an important role in saving the tooth. In Endodontics it is a routine treatment that involves removing the contaminated dentine, infected pulp, inflamed and dead pulp tissue by cleaning and shaping the canal with intracanal medication, disinfecting with irrigants to eliminate the all causative microorganisms. For the long-standing success of the endodontically treated tooth, it is necessary to eliminate persistent bacteria, such as Enterococcus faecalis. The ruinous components of Enterococcus Faecalis, including its capacity to tackle diverse microorganisms, attack dentinal tubules, and contradict nutritional difficulty. Using a good aseptic debridement procedure of diseased pulp and contaminated dentin with a combination of 2% chlorhexidine is the best way to eliminate E. faecalis in root canal procedure. Following a systematic review and metanalysis, the efficacy of 2% chlorhexidine (CHX) on Enterococcus faecalis was evaluated. Aim: The goal of this review was to determine and compare the efficacies of root canal disinfectant 2% chlorhexidine with 5.25% sodium hypochlorite (NaOCl), Calcium hydroxide (Ca(OH) 2),17% EDTA, MDTA. Materials and Methods: Literature research is performed in sources of data like MEDLINE, online library journal PubMed NIH, NCBI, WILEY ONLINE LIBRARY, ORAL HEALTH GROUP, Scopus ETC. Electronic information bases had been looked through utilizing the resulting keywords: Root canal irrigants, 2% chlorhexidine, Enterococcus faecalis, Calcium hydroxide NaOCl Root canal irrigation medicine. Our search was done on articles distributed to date. Results: After reviewing the titles and abstracts, many articles met the eligibility. The use of chemical agents with 5.25 percent sodium hypochlorite and 2 percent chlorhexidine resulted in microbial eradication at 100 percent compared to using only 5.25% sodium hypochlorite alone or calcium hydroxide alone which decreased by 97.7 percent compared to baseline microbial count and reduced by 68.42 percent at the same dilutions after mechanical cleaning respectively.. Conclusion: In this review study,2% Chlorhexidine has greater disinfection than calcium hydroxide and 5.25% sodium hypochlorite.
Antimicrobial Susceptibility of Enterococcus faecalis Isolated From Root Canal: An In Vitro Study
Avicenna Journal of Pharmaceutical Research
Background: One of the major causes of failure in root canal treatment is microorganisms like Enterococcus faecalis. Methods: This study aimed to examine the effectiveness of triple antibiotic paste (TAP) containing ciprofloxacin, metronidazole, and minocycline in comparison with modified TAP (MTAP) containing metronidazole, ciprofloxacin, and clindamycin and calcium hydroxide on Enterococcus strains isolated from root canal of tooth associated with pre-apical lesion. Moreover, we evaluated the E. faecalis resistance and antibiotic susceptibility. To do so, 25 patients with previous failure of endodontic treatment were enrolled in this study. Results: The antimicrobial effect of MTAP10%, MTAP1%, TAP10%, TAP1%, and CaOH was significantly better than the positive control group (P <0.05). There was no significant difference between antimicrobial effect of TAP1% and MTAP1%, and CaOH was the least effective one. The antimicrobial effect of TAP10% was significantly better than MTAP10% ...
New intracanal formulations containing doxycycline or chlorhexidine against Enterococcus faecalis
The journal of contemporary dental practice, 2014
The present study aims to evaluate the antimicrobial effect of two new intracanal preparations against E. faecalis. Thirty single-rooted human canine teeth were used. The crowns were removed and the roots were instrumented using a conventional technique. Three groups of ten teeth each were infected with 108 CFU/ ml of E. faecalis for 21 days. The root canals were flled with new intracanal medications containing 3% doxycycline hydrochloride (DX) or 2% chlorhexidine digluconate (CHX). Ten teeth received no medication (NM)-negative control. Microbial samples were obtained 21 days after contamination: 14 days under the effect of the intracanal medications and 7 days after replacing the medications by BHI broth. The samples were homogenized, diluted, seeded on BHI agar and incubated for 48h/36°C. The number of colony forming units (CFU/ml) was obtained and analyzed statistically. All intracanal dressings significantly reduced the number of bacterial cells in the root canal after 14 days ...
Journal of endodontics, 2006
The purpose of this in vitro study was to evaluate the antimicrobial effects of five antibiotics when added to Kerr Pulp Canal Sealer EWT against Enterococcus faecalis. Five antibiotics: amoxicillin, penicillin, clindamycin, metronidazole, and doxycycline, were added separately to Kerr sealer. Thirty brain heart infusion agar plates were inoculated with E. faecalis and sterile paper discs containing a sealer-antibiotic combination were randomly assigned to the inoculated plates. Kerr sealer on a sterile paper disc and a blank sterile paper disc served as the controls. Fifteen plates were incubated aerobically and the remaining 15 plates were incubated anaerobically at 37 degrees C. The zones of inhibition were measured at 48 hours. No significant differences (p > 0.05) were found between aerobic and anaerobic groups. The groups were combined and data analysis using a one-way ANOVA and Tukey's post hoc test was performed. Results revealed that sealer-antibiotic combinations co...
A New Irrigant against E. faecalis in Root Canal Disinfection
Biosciences Biotechnology Research Asia, 2014
Non surgical endodontic therapy uses biomechanical cleansing and shaping of the canal, which may be combined with the application of antimicrobial irrigants and intracanal medications, to eliminate these bacteria. The study evaluated the antimicrobial efficacy of Ocetenidine Dihydrochloride, a newer irrigant with Sodium Hypochlorite, a gold standard against Enterococcus faecalis in root canal disinfection. 30 single rooted intact pre-molar teeth were collected. The teeth were decoronated to measure 7mm long segments. The root apexs were enlarged to size 40 using K files. The root apex was sealed using light cure composite resin and surface was coated with nail varnish. Following sterilization of the blocks, they were infected with pure cultures of E.faecalis, which was grown in Muller-Hinton agar for a period of 7 days. The specimens were divided into 3 distinctive groups containing 10 teeth each: Group I with Octenidine, (0.1%),Group II with NaOCL (5%),Group III with no irrigation (control). Each block was carefully removed and was irrigated with 3ml of the irrigant for 1 min. After irrigation, the dentine debris were harvested using Gates Glidden drills No.2 and No.3. The antibacterial assessment was carried out by finding out the MIC and MBC values by serial dilution method, and the proportions of viable bacteria under epifluorescence microscope using Syto9 dye. Statistical analysis comparing the inter-group was done using MANN-WHITNEY U test and KRUSKAL-WALLIS test. From the results it was observed that the antimicrobial efficacy of Octenidine was found to be higher than 5% Sodium hypochlorite against E.faecalis and in creating a "bacteria-free" root canal system.
The Journal of Contemporary Dental Practice, 2013
Aim This study was conducted to evaluate the antimicrobial activity of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) and BioPure MTAD when used as a final rinse against Enterococcus faecalis. Materials and methods Sixty single-rooted premolars were biomechanically prepared, inoculated with E. faecalis and divided into various groups. These were then irrigated with the test irrigants and tested microbiologically for growth of E. faecalis immediately after irrigation and after 48 hours. Results Statistical analysis showed that there was a significant difference between the antibacterial activities of BioPure MTAD, 2% CHX and 5.25% NaOCl at 5 minutes; however, the antibacterial activities of the three irrigants were comparable after 2 days of irrigation Conclusion The present study concludes that BioPure MTAD is as effective against E. faecalis as 5.25% NaOCl and more effective than 2% CHX. Clinical significance E. faecalis is one of the most resistant intracanal species an...
Intracanal Antimicrobial against Enterococcus Faecalis
Aims: The efficacy of sodium hypochlorite associated with either chlorhexidine or calcium hydroxide, in reducing E. faecalis counts was evaluated by an in vitro infection-induced model. Materials and Methods: Thirty-four root-canal-treated human molar teeth were infected with E. faecalis for 14 days. After this period, 13 teeth were treated with chlorhexidine; 13 teeth with calcium hydroxide; and, eight teeth with saline. The teeth were sampled immediately after chemo-mechanical preparation, 14 days after intracanal medication, and 7 days without any intracanal antimicrobial. Results: A significant reduction of the number of E. faecalis within the root canal was observed with hypochlorite sodium plus calcium hydroxide or chlorhexidine, after 14 days of use. Conclusions: These antimicrobial associations can be use during endodontic treatment.
Root Canal Irrigation: Chemical Agents and Plant Extracts Against Enterococcus faecalis
The Open Dentistry Journal, 2016
Background:There are various microorganisms related to intra and extra-radicular infections and many of these are involved in persistent infections. Bacterial elimination from the root canal is achieved by means of the mechanical action of instruments and irrigation as well as the antibacterial effects of the irrigating solutions.Enterococcus faecaliscan frequently be isolated from root canals in cases of failed root canal treatments. Antimicrobial agents have often been developed and optimized for their activity against endodontic bacteria. An ideal root canal irrigant should be biocompatible, because of its close contact with the periodontal tissues during endodontic treatment. Sodium hypoclorite (NaOCl) is one of the most widely recommended and used endodontic irrigants but it is highly toxic to periapical tissues.Objectives:To analyze the literature on the chemotherapeutic agent and plant extracts studied as root canal irrigants. In particularly, the study is focused on their ef...