Produce Spherical Nanoparticles of Poly (butyl cyanoacrylate) as Nano Carrier by Emulsion Polymerization Method (original) (raw)
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Poly Alkyl Cyanoacrylate Nanoparticles as Delivery Vehicle in Combating Diseases
INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH, 2020
The major problems in drug delivery to specific diseased sites possess drug-resistance, drug-toxicity, biological barriers and non-specificity to the target-cells observed among patients worldwide. To overcome these barriers, the biodegradable poly alkyl cyanoacrylate (PACA) nanocarriers have been used, while a core made up of PACA is functionalized with biologically active ligands such as cargos, biotin and antibody for enabling specific targeting, and stabilized with outer polyethylene glycol or surfactants in combating cancer, neurodegenerative and infectious diseases. The review demonstrates mainly the synthesis, functionalizations and the biomedical applications of PACA nanoparticles against various diseases to consider them as potent delivery vehicle.
Asian Pacific journal of cancer prevention : APJCP, 2017
Cancer is one of the most important issues in modern medicine and the most common cause of death after cardiovascular diseases in many countries. Brain cancer is one of the most common causes of cancer death among men and women, ranking third. Chemotherapeutic drugs that aim to prevent uncontrolled proliferation of cells in tissues of the body and induce apoptosis of tumor cells are prominent candidates for development. Since cisplatin has an apoptosisinducing role, it is widely used as an anticancer agent. In this research, toxicity of cisplatin was studied with the C6 rat glioma cell lined using the MTT method. In addition, nanoparticles underwent SEM microscopic imaging. Particle average size, size distribution, polydispersity index (PDI) and zeta potential of poly butyl cyanoacrylate nanoparticles were found to be 222 nm, 0.470 ± 0.04 and 5.1 ± 0.2 mV, respectively. The results showed that nanoconjugates of cisplatin have more cytotoxic effects on C6 cells than the free drug (P&...
Colloid and Polymer Science, 2010
Classically, drug-loaded poly(alkylcyanoacrylate) colloidal carriers are prepared by the drug entrapment during emulsion polymerization. However, a number of chemically sensitive drugs are unstable in the conditions of polymerization or can be irreversibly inactivated by the highly reactive monomer. Furthermore, the particle size distribution and the molecular weight of formed polymer depend strongly on the polymerization conditions. Here, we investigate the nanoprecipitation approach for the preparation of pure and drug-load poly(butylcyanoacrylate) nanoparticles. This method allows the successful entrapment of lipophilic and chemically labile drugs by avoiding the contact with highly reactive monomers. The anticancer agent chlorambucil is chosen as the model drug for the incorporation and release studies. Pure and drug-loaded nanoparticles are successfully prepared using various stabilizers (Polysorbate 80, Pluronic F68, Dextran 40). The nanoparticles coated with Polysorbate 80 are of highest interest since they could overcome the blood–brain barrier and the multidrug resistance in cancer cells. Such nanoparticles can be easily prepared by the nanoprecipitation approach reported here.
The colloidal particles of poly(alkyl cyanoacrylate) (PACA) posses a great promise as drug carrier systems for delivery of anticancer drugs, antibiotics and other pharmaceuticals. Such particles are classically prepared by emulsion polymerization, starting from the respective monomer. Our recent studies demonstrate that pure and drug-loaded PACA colloidal particles could be successfully prepared by nanoprecipitation, starting from a presynthesized polymer. In this report, we consider the advantages and disadvantages of both methods (nanoprecipitation and polymerization) for the preparation of PACA colloids, loaded with anticancer drugs. The effects of preparation method on the characteristics of as-obtained drug-loaded PACA colloids are described. The possibilities for application of such particles for cancer treatment are discussed.
Cellular uptake and intracellular degradation of poly(alkyl cyanoacrylate) nanoparticles
Journal of nanobiotechnology, 2016
Poly(alkyl cyanoacrylate) (PACA) nanoparticles have shown promise as drug carriers both to solid tumors and across the blood-brain barrier. Efficient drug delivery requires both high cellular uptake of the nanoparticles and release of the drug from the nanoparticles. Release of hydrophobic drugs from PACA nanoparticles is primarily governed by nanoparticle degradation, and this process has been poorly studied at the cellular level. Here we use the hydrophobic model drug Nile Red 668 (NR668) to investigate intracellular degradation of PACA nanoparticles by measuring changes in NR668 fluorescence emission and lifetime, as the spectral properties of NR668 depend on the hydrophobicity of the dye environment. We also assess the potential of poly(butyl cyanoacrylate) (PBCA) and poly(octyl cyanoacrylate) (POCA) nanoparticles for intracellular drug delivery in the prostate cancer cell line PC3 and rat brain endothelial cell line RBE4 and the role of endocytosis pathways in PACA nanoparticle...
2016
The main purpose of this studywas preparation of a pharmaceutical formulation with etoposide loaded Poly Butyl Cyano Acrylate (PBCA) nanoparticles and evaluation of its effect on brain cancer. Etoposide loaded nanocarrier was synthesized using micro-emulsion polymerization method and characterized for shape morphology, particle size, zeta potential and drug-release properties. In the next step, BE (2)-C brain cell line was used to determine the rate of nanocarrieretoposide cytotoxicity. Etoposide loaded nanoparticles were characterized and size and zeta potential of nanoparticles containing drugs were measured as 225.3±21.7 nm and -26.3 ±1.5 mv, respectively. Drug loading and encapsulation values were estimated 8.7±0.15 and 97.3±2.1%, respectively. The release amount of drug from PBCA nanoparticles indicated the retention strength corresponding to the formulation. Cytotoxicity studies showed that the cell toxicity effect of drug loaded PBCA nanoparticles is almost two times higher t...
2021
We have investigated the biodistribution and tumor macrophage infiltration after intravenous injection of the poly(alkyl cyanoacrylate) nanoparticles (NPs): PEBCA (poly(2-ethyl-butyl cyanoacrylate), PBCA (poly(n-butyl cyanoacrylate), and POCA (poly(octyl cyanoacrylate), in mice. These NPs are structurally similar, have similar PEGylation, and have previously been shown to give large variations in cellular responses in vitro. The PEBCA NPs had the highest uptake both in the patient-derived breast cancer xenograft MAS98.12 and in lymph nodes, and therefore, they are the most promising of these NPs for delivery of cancer drugs. High-resolution magic angle spinning magnetic resonance (HR MAS MR) spectroscopy did not reveal any differences in the metabolic profiles of tumors following injection of the NPs, but the PEBCA NPs resulted in higher tumor infiltration of the anti-tumorigenic M1 macrophages than obtained with the two other NPs. The PEBCA NPs also increased the ratio of M1/M2 (an...
Drug delivery to resistant tumors: the potential of poly(alkyl cyanoacrylate) nanoparticles
Journal of Controlled Release, 2003
Simultaneous cellular resistance to multiple lipophilic drugs represents a major problem in cancer chemotherapy. This drug resistance may appear clinically either as a lack of tumor size reduction or as the occurrence of clinical relapse after an initial positive response to antitumor treatment. The resistance mechanism can have different origins either directly linked to specific mechanisms developed by the tumor tissue or connected to the more general problem of distribution of a drug towards its targeted tissue. The purpose of this paper is to summarize the results of the use of poly(alkyl cyanoacrylate) nanoparticles to overcome multidrug resistance (MDR) phenomena at both the cellular and the non-cellular level.
Synthesis and In vitro Cytotoxicity of a novel efficient Cisplatin-loaded poly N-butyl cyanoacrylate
Purpose: Typically, drug-loaded polymer colloidal carriers are synthesized by the drug entrapment during anionic emulsion polymerization. The purpose of this study was to trial the ability of Cisplatin-loaded Poly Butyl Cyanoacrylate (PBCA) nanoparticles. Methods: The cytotoxicity of Cisplatin-loaded PBCA nanoparticles was evaluated by MT assay. The Polymeric nanoparticles have been characterized using TEM, SEM, FTIR and DLS. Results: Polymeric nanoparticles with loaded and unloaded drug prepared by this method, have spherical structure with 350 and 230 nm in diameter and their zeta potentials were -3.2 and -10.7 mv respectively. The average amount of drug loaded on the nanoparticles was 70.88 ± 5.51%. MTT assay was carried out to evaluate the cytotoxicity property of the nanoparticles. Inhibition concentration values (IC 50 ) for Cisplatin-loaded Nanoparticles were 20.8 and 18.2 μM for 24 and 48 h respectively. Conclusion: It was shown that cisplatin-loaded nanoparticles strongly increased cytotoxicity in comparison to classic drug in the MCF-7 cell line.