Synthesis and characterization of curcumin loaded polymer/lipid based nanoparticles and evaluation of their antitumor effects on MCF-7 cells (original) (raw)
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Nanomaterials
Cancer remains a heavy health burden resulting in a high rate of mortality around the world. The presently used anticancer drugs suffer from several shortcomings, such as drug toxicity, poor biodegradability and bioavailability, and poor water solubility and drug resistance. Cancer is treated effectively by combination therapy whereby two or more anticancer drugs are employed. Most of the combination chemotherapies result in a synergistic effect and overcome drug resistance. Furthermore, the design of polymer-based nanocarriers for combination therapy has been reported by several researchers to result in promising therapeutic outcomes in cancer treatment. Curcumin exhibits good anticancer activity but its poor bioavailability has resulted in its incorporation into several polymer-based nanocarriers resulting in good biological outcomes. Furthermore, the incorporation of curcumin together with other anticancer drugs have been reported to result in excellent therapeutic outcomes in vi...
Al-Azhar Journal of Pharmaceutical Sciences, 2015
Curcumin has a wide spectrum of biological and pharmacological activities such as anti-inflammatory and anticancer activities but its main drawbacks (Low stability and poor bioavailability profiles) have restricted its therapeutic applications. In our previous study an optimized formula of solid lipid nanoparticles containing curcumin (C-SLNs) was prepared to overcome its drawbacks. The aim of the optimized C-SLN formula to increase curcumin's stability, and enhance its anticancer activity against A2780 ovarian cancer cells. The purpose of the current study was characterization of the optimized C-SLN formula through conducting morphological examination, stability, cytotoxicity and cellular uptake studies. The morphological examination using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) revealed the spherical nature of the optimized C-SLN formula. The stability of the optimized C-SLN formula at different temperatures was evaluated over the storage period of 6 months. The cytotoxicity and cellular uptake studies revealed enhanced antiproliferative activity of the optimized C-SLN formula compared to native curcumin solution in DMSO. The present study indicated the effectiveness of C-SLNs in enhancing the anticancer effect of curcumin in ovarian cancer cells in-vitro.
Pakistan journal of pharmaceutical sciences, 2014
Curcumin (diferuloylmethane) possesses low bioavailability due to its poor solubility, permeability and rapid metabolism. Solid Lipid Nanoparticle of curcumin was prepared by high-speed homogenization technique. Stearic acid was used as a lipid, tween 80 as surfactant and various co surfactants were used for the preparation of SLN. The prepared SLN was characterized using zeta sizer, TEM analysis and the average particle size was found to be in the range of 80 nm - 200nm. The entrapment efficiency of the SLN was ~58 to 85%. The characteristic FTIR peaks suggest that the stearic acid is compatible with curcumin. MTT assay was performed on the optimized formulation and the results are indicative that curcumin SLN showed better cytotoxicity in low dose while compared to plain curcumin. The developed Cu-SLN can find its better place in the anticancer therapy.
International Journal of Current Pharmaceutical Research, 2022
Objective: The present study was aimed at preparing stable lyophilized curcumin loaded nanostructured lipid carriers (NLCs). The optimized lyophilized curcumin loaded NLCs were characterized and evaluated for various quality control parameters. Methods: The optimized curcumin loaded NLCs were prepared by modified hot emulsification using precirol ATO 5 (PRE), capmul MCM C8 EP (CAP) as solid and liquid lipids, respectively. The combination of tween 80 (T80) and solutol HS 15 (SHS) were used as an emulsifier. The NLCs dispersion was lyophilized into powder form to improve the thermodynamic stability of the formulation. The lyophilized curcumin loaded NLCs were evaluated for particle size, size distribution, zeta potential, entrapment efficiency (EE), drug loading, assay, in vitro drug release, crystallinity and surface morphology studies. Results: The optimized lyophilized curcumin loaded NLCs have a mean particle size of 286.2±11.5 nm with a size distribution of 0.288±0.011, a zeta p...
Effect of Polymeric Nanoparticles of Curcumin on A549 Cell Line
International Journal of Current Pharmaceutical Research
Objective: Lung cancer cell line specially A549 is one of the most common malignant tumors. Curcumin is obtained from rhizomes of curcuma longa and does possess biological and pharmacological properties, among them anti-tumor activity is also one of the major findings. Here we try to developed a method for delivery of curcumin for cancer therapy. Methods: Due to low water solubility and bioavailability curcumin delivery designed as polymeric nanoparticles and prepared by emulsification homogenization method. Results: Characterization done by evaluating zeta size, potential, scanning electron microscopy. Release pattern also studied along with encapsulation and loading data. Nanoparticles were found to be spherical from morphological study and found to be below 200 nm. It does possess cytotoxic activity against lung cancer cell line and potent in comparison with its free from. Conclusion: We can conclude that polymeric nanoparticles are suitable form to delivery for antitumor activit...
Journal of Biomedical Nanotechnology, 2011
Curcumin is a polyphenol obtained from the plant Curcuma longa (called turmeric) that displays several pharmacological activities, including anti-inflammatory, antioxidant, antimicrobial and antitumoral activity, but clinical use has been limited by its poor solubility in water and, consequently, minimal systemic bioavailability. We have therefore formulated the drug into nanocarrier systems in an attempt to improve its therapeutic properties. This study evaluates the effect of intraperitoneally administered nanocapsules containing curcumin on subcutaneous melanoma in mice inoculated with B16-F10 cells, and on the cytotoxicity activity against B16-F10 cells in vitro. Phagocytic uptake of formulations was also evaluated upon incubation with macrophage J774 cells by fluorescence microscopy. Lipid and polymeric nanocapsules were prepared by the phase inversion and nanoprecipitation methods, respectively. The uptake of the lipid nanocapsules prepared using Solutol HS15 was significantly reduced in J774 cells. Curcumin, as free drug or as drug-loaded nanocapsules, was administrated at a dose of 6 mg/kg twice a week for 21 days. Free drug and curcuminloaded nanocapsules significantly reduced tumor volume (P < 0.05 vs. control), but no difference was found in the antitumor activity displayed by lipid and polymeric nanocapsules. This assumption was supported by the in vitro study, in which free curcumin as well as loaded into nanocapsules caused significant reduction of cell viability in a concentration-and time-dependent manner.
Journal of agricultural and food chemistry, 2017
Curcumin is a natural molecule with proved anticancer efficacy on several human cancer cell lines. However, its clinical application has been limited due to its poor bioavailability. Nanocarrier-based drug delivery approaches could make curcumin dispersible in aqueous media, thus overtaking the limits of its low solubility. The aim of this study was to increase the bioavailability and the antitumoral activity of curcumin, by entrapping it into nanostructured lipid carriers (NLCs). For this purpose here we describe the preparation and characterization of three kinds of curcumin-loaded NLCs. The nanosystems allowed the achievement of a controlled release of curcumin, the amounts of curcumin released after 24 h from Compritol-Captex, Compritol-Miglyol, and Compritol NLCs being, respectively, equal to 33, 28, and 18% w/w on the total entrapped curcumin. Considering the slower curcumin release profile, Compritol NLCs were chosen to perform successive in vitro studies on ovarian cancer ce...
Journal of biomedical nanotechnology, 2011
Curcumin is a polyphenol obtained from the plant Curcuma longa (called turmeric) that displays several pharmacological activities, including anti-inflammatory, antioxidant, antimicrobial and antitumoral activity, but clinical use has been limited by its poor solubility in water and, consequently, minimal systemic bioavailability. We have therefore formulated the drug into nanocarrier systems in an attempt to improve its therapeutic properties. This study evaluates the effect of intraperitoneally administered nanocapsules containing curcumin on subcutaneous melanoma in mice inoculated with B16-F10 cells, and on the cytotoxicity activity against B16-F10 cells in vitro. Phagocytic uptake of formulations was also evaluated upon incubation with macrophage J774 cells by fluorescence microscopy. Lipid and polymeric nanocapsules were prepared by the phase inversion and nanoprecipitation methods, respectively. The uptake of the lipid nanocapsules prepared using Solutol HS15 was significantly reduced in J774 cells. Curcumin, as free drug or as drug-loaded nanocapsules, was administrated at a dose of 6 mg/kg twice a week for 21 days. Free drug and curcuminloaded nanocapsules significantly reduced tumor volume (P < 0.05 vs. control), but no difference was found in the antitumor activity displayed by lipid and polymeric nanocapsules. This assumption was supported by the in vitro study, in which free curcumin as well as loaded into nanocapsules caused significant reduction of cell viability in a concentration-and time-dependent manner.
Journal of Cancer and Tumor International, 2021
Aims: The present study was aimed at preparing stable lyophilized curcumin loaded nanostructured lipid carriers (NLCs). The optimized lyophilized curcumin loaded NLCs were characterized and evaluated for various quality control parameters. Methodology: The optimized curcumin loaded NLCs were prepared by modified hot emulsification using compritol 888 ATO (CMPR), capmul MCM C8 EP (CAP) as solid and liquid lipids respectively. The combination of tween 80 (T80) and solutol HS 15 (SHS) were used as an emulsifier. The NLCs dispersion was lyophilized into powder form to improve the thermodynamic stability of the formulation. The lyophilized curcumin loaded NLCs were evaluated for particle size, size distribution, zeta potential, entrapment efficiency (EE), drug loading, assay, in-vitro drug release, crystallinity, thermal behavior and surface morphology studies. Results: The optimized lyophilized curcumin loaded NLCs have a mean particle size of 332.88 ± 5.9 nm with a size distribution of...
Anticancer Drug Delivery Systems Based on Curcumin Nanostructures: A Review
Pharmaceutical Chemistry Journal, 2020
In recent years, the application of nanostructures in biomedical and pharmaceutical fields has increased. The special designs and compositions make nanocomposites very useful alternatives to conventional materials. Curcumin is a promising anti-cancer agent that has a positive and significant effect on chemotherapeutic achievements. The anticancer properties of curcumin have been widely investigated in different forms such as nanoparticles and nanocomposite structures. Chitosan-based nanocomposites, magnetic nanoparticles, polymer nanocomposites and blends, and montmorillonite-and alginate-based nanocomposites have been used in loading curcumin for various purposes. The anticancer preparations of curcumin nanoparticles and drug release systems employing curcumin-loaded nanoparticles, electrospun nanofibers, and hydrogel nanocomposites have been investigated. This review provides a summary of the applications of nanostructures containing curcumin, especially in controlled drug release systems. The curcumin nanoparticles and nanocomposites are suitable candidates for anticancer applications. On the nano-scale, curcumin has better aqueous solubility and, if used in a nanocomposite, there is a good ability for manipulating the drug delivery system properties.