Peptidergic innervation of blood vessels and interstitial cells in the testis of the cat (original) (raw)
Journal of andrology
We studied the innervation of the cat testis using a panel of antisera against the following neuronal markers: protein gene product 9.5 (PGP), neuropeptide Y, C-terminal peptide of neuropeptide Y, galanin, vasoactive intestinal peptide (VIP), calcitonin gene-related peptide, and substance P. Immunoreactivity against PGP, a general neuronal label, demonstrated the arrangement of fibers from the superior spermatic nerve (SSN) in the testicular pedicle and the cephalic testicular pole, and those of the inferior spermatic nerve (ISN) along the vas deferens and the inferior testicular ligament. The testicular parenchyma exhibited a very rich innervation, mainly distributed to blood vessels and Leydig cell nests, but also in close association with seminiferous tubules. Numerous peptidergic fibers were present in the SSN and ISN, albeit in different proportions. Thus, VIP-immunoreactive fibers were almost absent in the SSN, but were the most abundant subpopulation of the ISN. The testicula...
Related papers
Elsevier, 2003
Summary. The distribution of autonomous nerves in the testis of the camel was studied by immunohistochemical methods. A total of 26 testes was collected during the different seasons of the year. As pan-neuronal markers, antibodies to protein gene product 9.5 and to ncurofilaments are superior to antibodies against neuron-specific enolase and acetylcholinesterase histochemistry for the description of the nerves in the camel testis. Testicular nerves reach the camel testis by three access-routes as (1) funicular contribution, (2) mesorchial contribution and (3) as caudal contribution. The main target for testicular nerves is the arterial vascular tree of the organ, whereas all veins of testis and pampiniform plexus are devoid of any innervation in the camel. In the wall of the arteries, the nerves form a plexus at the media-adventitia border. The density of the arterial plexuses increases along the vascular tree: smaller septal and mediastinal arteries are better innervated than albugineal arteries and the latter better than the A. testicularis. The nerves in the septula testis, in the mediastinum and between the Leydig cells show clear seasonal changes, being particularly abundant in autumn and particularly scarce in spring. The nerves that reach the camel testis are unmyelinated and represent in the vast majority postjunctional sympathetic neurons. Cholinergic fibers are absent in the camel testis. Neuropeptide Y is the dominating peptidergic transmitter in the testicufar nerves and colocalized with noradrenaline in the same axons. Vasoactive intestinal polypeptide-containing fibers reach the camel testis exclusively as parts of the caudal Correspondence to: K.-H. Wrobel E-mail: karl-heinz.wrobel@vkl.uni-regensburg.de nervous contribution via the ligamentous bridge between testis and epididymal tail and are restricted to the caudal pole of the testis. Calcitonin gene-related peptide-positive axons are not frequent in the camel testis; nevertheless, they seem to be the most important sensory pathway of this organ.
Cell and Tissue Research, 1995
The presence and distribution of peptidergic nerve fibers were studied in the testis and mesorchium of the toad by means of immunohistochemistry. Cryostat sections of the testis and whole-mount preparations of mesorchia were immunostained with antisera to calcitonin gene-related peptide (CGRP) and neuropeptide tyrosine (NPY). After leaving the mesorchium CGRP-immunoreactive (IR) fibers were seen predominantly running in between the seminiferous tubules. In addition, a small population of CGRP-IR nerve fibers formed thin plexuses around blood vessels. Conversely, NPY-like immunoreactivity predominated in nerve fibers that formed dense plexuses around vessels both in the mesorchium and testis. Additionally, some single NPY-IR nerve fibers could be seen in both structures studied. The functional significance of these peptidergic systems in the testis of the toad remains to be analyzed.
Loading Preview
Sorry, preview is currently unavailable. You can download the paper by clicking the button above.