PHYTOCHEMICAL AND PHARMACOLOGICAL SELECTION OF RANUNCULUS MURICATUS AS MEDICINAL WEED FROM DISTRICT LAHORE, PAKISTAN. (original) (raw)
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PHYTOCHEMICAL STUDY OF AERIAL PARTS OF RANUNCULUS MURICATUS FOR THE PHARMACOLOGICAL ACTIVE COMPOUNDS
Phytochemical study for the pharmacological activity is an important segment of drug designing. This initial work establishes a foundation for product development. The pharmacological active compounds are finally developed into a pharmaceutical preparation. This is used in actual clinical and therapeutical practice to cure miscellaneous pathological problems. Thus; we have aimed this study for the extraction of compounds using two solvents of different polarities, so that maximum number of compounds can be extracted. Analytical technique used for the isolation and identification of the various compounds is thin layer chromatography. Phytochemical analysis of aerial parts showed the presence of Tannins, saponin and cardiac glycosides. Cardioactive glycosides, Tannin and saponin were reported in aerial parts of Ranunculus muricatus for the first time. Therefore Ranunculus muricatus can be used for further isolation and structural determination of cardioactive compounds.
ANTIFUNGAL, CYTOTOXIC AND PHYTOTOXICITY OF AERIAL PART OF RANUNCULUS MURICATUS
OBJECTIVE: The present research is preliminary biological screening of aerial plant of Ranunculus muricatus (Ranunculaceae). Dichloromethane and methanol extracts of the aerial plant were investigated for their antifungal, phytotoxic and cytotoxic activities. METHOD: Anti-fungal, cytotoxicity and phytotoxicity activity was performed by Agar tube dilution assay, Brine shrimp lethality bioassay and lemna bioassay respectively. RESULT: Dichloromethane and methanolic extract exhibited significant phytotoxicity against Lemna minor having Paraquat as standard drug and incubation condition (28 ± 1ºC). None of extracts presented any significant cytotoxic activity having Imipenum and Etoposide as standard drug respectively. Both extract had non-significant antifungal activity but it has been noted that methanol extract showed 30% inhibition with linear growth at 70mm, when compared with control; only against Microsporum canis. CONCLUSION: Ranunculus muricatus showed significant phytotoxicity. The phytotoxicty assay is a valuable major screen for weedicide investigation. Additionally, modern studies are currently carried out to identify the allelopathic constituents by isolation, purification and structure elucidation to find out as effective herbicidal.
Antioxidant and phytochemical analysis of Ranunculus arvensis L. extracts
BMC research notes, 2015
Ranunculus arvensis L. (R. arvensis) has long been used to treat a variety of medical conditions such as arthritis, asthma, hay fever, rheumatism, psoriasis, gut diseases and rheumatic pain. Here, we screened R. arvensis for antioxidant activity, phytochemical and high performance liquid chromatography (HPLC) analyses. The chloroform, chloroform:methanol, methanol, methanol:acetone, acetone, methanol:water and water extracts of R. arvensis were examined for DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radical scavenging assay, hydrogen peroxide scavenging assay, phosphomolybdenum assay, reducing power assay, flavonoid content, phenolic content and high performance liquid chromatography analysis. Significant antioxidant activity was displayed by methanol extract (IC 50 34.71 ± 0.02) in DPPH free radical scavenging assay. Total flavonoids and phenolics ranged 0.96-6.0 mg/g of extract calculated as rutin equivalent and 0.48-1.43 mg/g of extract calculated as gallic acid equivalent respec...
International Journal of Research in Pharmaceutical Sciences
Ranunculus sceleratus Linn. is one of the well-known medicinal plants, being used from the ancient time in India and commonly called as “Jal dhaniya”. It belongs to Ranunculaceae family derived from two Latin Words “Rana” means frog and “unculus” means little and referred together as “little frog” and also species sceleratus for cursed. It is an aquatic perennial herb. It consists of a herbaceous hollow stem, firm tap root, branched rhizome and leaves having a smooth upper side. The green plant is toxic for livestock and uncomfortable to human skin. This plant can grow up to 0.60 m tall, and also used as a food, medicine and possess other uses. Ranunculus sceleratus Linn. is commonly spread in the temperate and cold region in Global distribution (Indonesia, Malaysia, Nepal, Sri Lanka and India). According to Bentham and Hooker classification “Genera Plantarum”, this plant belongs to the division Polypetale of Dicotyledones which processes more than 600 species. The Phytochemical scr...
In Vitro Biological Activities Of Ranunculus gracilis Clarke Rhizome
Journal of anatolian environmental and animal sciences, 2022
Ranunculus gracilis rhizomes were picked from Yenice Forests Karabük province in Turkey. Ethanol was chosen for extraction solvent. Disc diffusion method including filamentous and non-filamentous Gram-positive bacteria, Gram-negative bacteria, and yeast strains was used to evaluate the antimicrobial activity of the extract. 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and ferric reducing antioxidant power analysis were performed to determine antioxidant activity. The Folin-Ciocalteu method for determining the total phenolic amount and the AlCl3 method for the total flavonoid content of the extract was chosen. Mean diameters of inhibition zones (IZD) of the bacteria were found in the range of 8.2 mm to 24.45 mm. This value was measured as 17.82 mm and 18.69 mm for yeasts. The total antioxidant activity value of the extract was calculated as 7.08 mg AAE/g extract. The IC50 value was found as 9.097 mg/mL for DPPH free radical scavenging activity. The FRAP value indicated that the reducing power of 1 gram of sample was equivalent to 4.66 μmol of Trolox. The total phenolic content of ethanol extract of R. gracilis rhizomes was determined as 0.414 mg GAE/g, while the flavonoid content was calculated as 0.68 mgQE/g. This study is the first report demonstrating the biological activities of R. gracilis in the literature. The analyzed ethanolic extract of R. gracilis rhizomes demonstrated that the biological activity level could be considered significant according to the obtained results.
Habitats, phytochemical metabolites and phytoremediation potential of Ranunculus sceleratus L
Journal of Pure & Applied Sciences
Ranunculus sceleratus L. (Ranunculaceae) is an invasive alien widespread species commonly distributed in canals and drains banks. In Egypt, this plant exhibits extensive growth with occasional pure populations in the Nile Delta region. This study aimed to address the drivers for R. sceleratus abundance, phytochemical composition, and phytoremediation efficiency. The average abundance of R. sceleratus within 60 stands of 25 m2 each was 47.9. The average root and stem lengths were 31.5 and 60.1 cm, respectively. The abundance of R. sceleratus was positively correlated with pH, electric conductivity, organic carbon, Fe, Mn, and Zn, while negatively correlated with total dissolved phosphorus, Cu, Co, Cd, and Pb. The shoot had the highest values of all bioactive-metabolites, where phenols, flavonoids, tannins, alkaloids and saponins were recorded 23.16, 5.11, 14.78, 6.34 and 18.50 mg kg-1 dry weight, respectively. R. sceleratus had shoot and root bioaccumulation factors (BF) in the follo...
2010
Ranunculus arvensis, belongs to family Ranunculacea, have 2500 species throughout the world. Due to their local importance for various diseases and great biological potentials the plant was selected for further studies. Antioxidant activity was determined by DPPH scavenging method while phytotoxcicity was carried out through autoclaved petriplates growth inhibition. Antibacterial activity analysis was carried out by using microplates alamar blue assay. The Antioxidan, phytotoxic and antimicrobial results showed that this plant need detail phytochemical screening to isolate active chemical constituents which could be of scientific values.
Antimicrobial and Synergistic Studies of Ranunculus Muricatus L. Against Some Indigenous Bacteria
2014
In the present study, antibacterial activity of the whole plant methanolic extract of Ranunculus muricatus L., was analyzed against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Bacillus pumilus, Salmonella typhimurium and Pseudomonas aeroginosa. Methanol was regarded as an excellent solvent for antimicrobial activity. It was observed as best bactericidal at a minimal inhibitory concentration (MIC) of 1-10 µg/ml against all the bacterial cultures viz. B. pumilus, B. subtilis, S. aureus, E. coli, P. aeroginosa and S. typhimurium. Synergistic antibacterial activity of methanolic extracts was tested with respect to solvent extract of leaves of Ricinus communis, Nerium oleander, Withania somnifera, whole plant of Heliotropiaum curassavicum and fruits of Citrullus colocynthis. Synergistical study revealed the best antibacterial activity against B. subtilis and B. pumilus at a level of 1 µg/ml except E. coli and S. aureus.
In vitro antiinflammatory and antioxidant potential of root extracts from Ranunculaceae species
South African Journal of Botany, 2017
Among the ethanol extracts of roots of 25 Ranunculaceous plant species traditionally used in treatment of various inflammatory diseases, Helleborus purpurascens demonstrated the most promising inhibitory activity towards COX-1, COX-2 and 5-LOX enzymes (IC 50 detected as 5.64, 10.33 and 3.84 μg/mL, respectively). GC-MS analysis of subfractions obtained from H. purpurascens extract revealed that a complex of fatty acids (especially αlinolenic acid) might be responsible for the observed antiinflammatory activity. The extract of Thalictrum minus was found to be selectively inhibiting COX-2 and 5-LOX enzymes (IC 50 = 49.68 and 76.47 μg/mL, respectively) but had no inhibitory effect on COX-1 (IC 50 N 128 μg/mL). Moreover, T. minus extract also showed significant antioxidant effect (DPPH = 0.074 g TE/g, and ORAC = 0.518 g TE/g) as well as was relatively rich on phenolic compounds (TPC = 0.352 g GAE/g). Statistical analysis showed that phenolic compounds might be only partially responsible for antioxidant potential demonstrated by this plant. The results of our study suggest that the above-mentioned members of Ranunculaceae family may possibly serve as a prospective material for further development of novel and safer plant-based antiinflammatory and/or antioxidant agents.