TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing (original) (raw)

Abstract

Aims: To evaluate the expression of transforming growth factor β1 (TGF-β1) and fibroblast growth factor 2 (FGF-2) mRNA in stromal cells in response to injury in the presence of either TGF-β1 or FGF-2. It has been shown previously that heparan sulfate proteoglycans and FGF-2 are present transiently during wound repair in vivo and that an increase in TGF-β1 mRNA is detected rapidly after injury. Methods: Primary corneal fibroblasts were cultured to confluency, serum starved, and linear wound(s) were made in medium containing TGF-β1 or FGF-2. TGF-β1 and FGF-2 mRNA expression were evaluated using both northern blot analysis and in situ hybridisation. Both dose dependent and time course experiments were performed. Whole eye organ culture experiments were also carried out and growth factor expression was assessed.

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References (49)

  1. Trinkaus-Randall V. Cornea. In: Lanza R, Langer R, Chick W, eds. Principles of tissue engineering. Austin, Texas: RG Landes Company, 1997:383-402.
  2. Trinkaus-Randall V. Cornea. In: Lanza R, Langer R, Chick W, eds. Principles of tissue engineering, 2nd ed. Boston: MA Academic Press, 2000:471-91.
  3. Brown CT, Vural M, Johnson M, et al. Age-related changes of scleral hydration and sulfated glycosaminoglycans. Mech Ageing Dev 1994;77:97-107.
  4. Brown CT, Nugent MA, Lau FW, et al. Characterization of proteoglycans synthesized by cultured corneal fibroblasts in response to transforming growth factor beta and fetal calf serum. J Biol Chem 1999;274:7111-19.
  5. Richardson TP, Trinkaus-Randall V, Nugent MA. Regulation of basic fibroblast growth factor binding and activity by cell density and heparan sulfate. J Biol Chem 1999;274:13534-40.
  6. Hassell JR, Cintron C, Kublin C, et al. Proteoglycan changes during restoration of transparency in corneal scars. Arch Biochem Biophys 1983;222:362-9.
  7. Cintron E, Gilula LA, Murphy WA, et al. The widened disk space: a sign of cervical hyperextension injury. Radiology 1981;141:639-44.
  8. Funderburgh JL, Chandler JW. Proteoglycans of rabbit corneas with nonperforating wounds. Invest Ophthalmol Vis Sci 1989;30:435-42.
  9. 9 Trinkaus-Randall V, Nugent MA. Biological response to a synthetic cornea. J Control Release 1998;53:205-14.
  10. Assoian RK, Komoriya A, Meyers CA, et al. Transforming growth factor-beta in human platelets: identification of a major storage site, purification, and characterization. J Biol Chem 1983;258:7155-60.
  11. Assoian RK, Fleurdelys BE, Stevenson HC, et al. Expression and secretion of type beta transforming growth factor by activated human macrophages. Proc Natl Acad Sci U S A 1987;84:6020-4.
  12. Gailit J, Welch MP, Clark RA. TGF-beta 1 stimulates expression of keratinocyte integrins during re-epithelialization of cutaneous wounds. Invest Dermatol 1994;103:221-7.
  13. Shipley GD, Pittelkow MR, Wille JJ, Jr, et al. Reversible inhibition of normal human prokeratinocyte proliferation by type beta transforming growth factor-growth inhibitor in serum-free medium. Cancer Res 1986;46:2068-71.
  14. Attisano L, Wrana JL, Lopez-Casillas F, et al. TGF-beta receptors and actions. Biochim Biophys Acta 1994;1222:71-80.
  15. Cross M, Dexter TM. Growth factors in development, transformation, and tumorigenesis. Cell 1991;64:271-80.
  16. Massague J. The transforming growth factor-beta family. Annu Rev Cell Biol 1990;6:597-641.
  17. Grotendorst GR, Smale G, Pencev D. Production of transforming growth factor beta by human peripheral blood monocytes and neutrophils. J Cell Physiol 1989;40:396-402.
  18. Kay EP, Lee MS, Seong GJ, et al. TGF-betas stimulate cell proliferation via an autocrine production of FGF-2 in corneal stromal fibroblasts. Curr Eye Res 1998;17:286-93.
  19. Massague J. TGF-beta signal transduction. Annu Rev Biochem 1998;67:753-91.
  20. Massague J. TGF-beta signaling: receptors, transducers, and Mad proteins. Cell 1996;85:947-50.
  21. Derynck R, Feng XH. TGF-beta receptor signaling. Biochim Biophys Acta 1997;1333:105-50.
  22. Nugent MA, Iozzo RV. Fibroblast growth factor-2. Int J Biochem Cell Biol 2000;32:115-20.
  23. Arese M, Chen Y, Florkiewicz RZ, et al. Nuclear activities of basic fibroblast growth factor: potentiation of low-serum growth mediated by natural or chimeric nuclear localization signals. Mol Biol Cell 1999;10:1429-44.
  24. Patry V, Bugler B, Maret A, et al. Endogenous basic fibroblast growth factor isoforms involved in different intracellular protein complexes. Biochem J 1997;326:259-64.
  25. Powell PP, Klagsbrun M. Three forms of rat basic fibroblast growth factor are made from a single mRNA and localize to the nucleus. Cell Physiology 1991;148:202-10.
  26. Song QH, Singh RP, Richardson TP, et al. Transforming growth factor-beta1 expression in cultured corneal fibroblasts in response to injury. J Cell Biochem 2000;77:186-99.
  27. Haq F, Trinkaus-Randall V. Injury of stromal fibroblasts induces phosphorylation of focal adhesion proteins. Curr Eye Res 1998;17:512-23.
  28. Winkles JA, Friesel R, Alberts GF, et al. Elevated expression of basic fibroblast growth factor in an immortalized rabbit smooth muscle cell line. Am J Pathol 1993;143:518-27.
  29. Grushkin-Lerner LS, Kewalramani R, Trinkaus-Randall V. Expression of integrin receptors on plasma membranes of primary corneal epithelial cells is matrix specific. Exp Eye Res 1997;64:323-34.
  30. Singer RH, Lawrence JB, Villnave C. Optimization of in situ hybridization using isotopic and nonisotopic detection methods. Biotechniques 1986;4:230-50.
  31. Song QH, Singh RP, Trinkaus-Randall V, et al. Injury and EGF mediate the expression of alpha6beta4 integrin subunits in corneal epithelium. J Cell Biochem 2001;80:397-414.
  32. Trinkaus-Randall V, Tong M, Thomas P, et al. Confocal imaging of the alpha 6 and beta 4 integrin subunits in the human cornea with aging. Invest Ophthalmol Vis Sci 1993;34:3103-9.
  33. Wu XY, Svoboda KK, Trinkaus-Randall V. Distribution of F-actin, vinculin and integrin subunits (alpha 6 and beta 4) in response to corneal substrata. Exp Eye Res 1995;60:445-58.
  34. Beales MP, Funderburgh JL, Jester JV, et al. Proteoglycan synthesis by bovine keratocytes and corneal fibroblasts: maintenance of the keratocyte phenotype in culture. Invest Ophthalmol Vis Sci 1995;40:1658-63.
  35. Clark Richard AF. Wound repair: overview and general considerations. In: Clark Richard AF, ed. The molecular and cellular biology of wound repair, 2nd ed. New York: Plenum Press, 1996:293-5.
  36. Sobrin L, Liu Z, Monroy DC, et al. Regulation of MMP-9 activity in human tear fluid and corneal epithelial culture supernatant. Invest Ophthalmol Vis Sci 2000;411:1703-9.
  37. Tervo T, van Setten GB, Paallysaho T, et al. Wound healing of the ocular surface. Ann Med 1992;24:19-27.
  38. Vesaluoma MH, Tervo TT. Tenascin and cytokines in tear fluid after photorefractive keratectomy. J Refract Surg 1998;14:447-54.
  39. Bennett NT, Schultz GS. Growth factors and wound healing: part II. Role in normal and chronic wound healing. Am J Surg 1993;166:74-81.
  40. Schultz GS, Strelow S, Stern GA, et al. Treatment of alkali-injured rabbit corneas with a synthetic inhibitor of matrix metalloproteinases. Invest Ophthalmol Vis Sci 1992;33:3325-31.
  41. Duncan MR, Frazier KS, Abramson S, et al. Connective tissue growth factor mediates transforming growth factor beta-induced collagen synthesis: down-regulation by cAMP. FASEB J 1999;13:1774-86.
  42. Nugent MA, Edelman ER. Transforming growth factor beta 1 stimulates the production of basic fibroblast growth factor binding proteoglycans in Balb/c3T3 cells. J Biol Chem 1992;267:21256-64.
  43. Nathan A, Nugent MA, Edelman ER. Tissue engineered perivascular endothelial cell implants regulate vascular injury. Proc Natl Acad Sci U S A 1995;92:8130-4.
  44. Andresen JL, Ledet T, Ehlers N. Keratocyte migration and peptide growth factors: the effect of PDGF, bFGF, EGF, IGF-I, aFGF and TGF-beta on human keratocyte migration in a collagen gel. Curr Eye Res 1997;16:605-13.
  45. Masur SK, Dewal HS, Dinh TT, et al. Myofibroblasts differentiate from fibroblasts when plated at low density. Proc Natl Acad Sci U S A 1996;93:4219-23.
  46. Petroll WM, Jester JV, Bean JJ, et al. Myofibroblast transformation of cat corneal endothelium by transforming growth factor-beta1, -beta2, and -beta3. Invest Ophthalmol Vis Sci 1998;39:2018-32
  47. Amento EP, Beck LS. TGF-beta and wound healing. Ciba Found Symp 1991;157:115-23.
  48. Roberts AB, Sporn MB. Physiological actions and clinical applications of transforming growth factor-beta (TGF-beta). Growth Factors 1993;8:1-9.
  49. Shah M, Revis D, Herrick S, et al. Role of elevated plasma transforming growth factor-beta1 levels in wound healing. Am J Pathol 1999;154:1115-24.