Simultaneous determination of methocarbamol and ibuprofen or diclofenac potassium using mean centering of the ratio spectra method (original) (raw)
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Analytical Chemistry Letters, 2016
A facile and accurate spectrophotometric method was developed for the simultaneous assay of ibuprofen (IBP) and methocarbamol (MTC) binary pharmaceutical mixture without prior tedious separation processes. The method was based on measuring the third and the fourth derivative amplitudes of each drug in presence of the other, using zero-crossing technique. The third derivative amplitudes were measured at 234.6 nm and at 226.8 nm for the simultaneous assay of MTC and IBP, respectively. On the other hand, the fourth derivative amplitude values were measured at 228 nm and at 233 nm for the simultaneous assay of MTC and IBP, respectively. The method was validated according to ICH guidelines. The linear calibration graphs were constructed over the concentration ranges of 3.0-30.0 and 5.0-50 μgmL-1 for MTC and IBP, respectively with sensitivities comparable to HPLC-UV methods. The detection limits of the third derivative measurements were 0.262 and 0.257 μgmL-1 for MTC and IBP, respectively, while for the fourth derivative measurements, the detection limits were 0.356 and 0.189 μgmL-1 for MTC and IBP, respectively. The proposed method was applied to assay the studied drugs in their pharmaceutical combined tablets. The results were efficiently compared to those obtained by the official USP reference methods.
Objective: Two simple, accurate, precise, reproducible and an economical spectrophotometric methods were developed for the simultaneous estimation of ibuprofen and famotidine in pharmaceutical bulk and synthetic mixture. Methods: The first method was developed on the basis of Q-absorbance ratio method (method I) for analysis of both the drugs. Wavelengths selected for analysis in Q-absorbance ratio method were 263 nm (λmax of ibuprofen) and 273.80 nm (iso-absorptive wavelength) in 0.1N NaOH. The second method was based on derivative spectrophotometric method (method II) involving the determination of both the drugs at their respective zero crossing point. The determinations were made at 252.8 nm (zero crossing point of famotidine) and 304 nm (zero crossing point of ibuprofen) in 0.1N NaOH. Results: Both the method obeys Beer-Lambert's law in the concentration range of 150-750 μg/ml for ibuprofen and 5-25 μg/ml for famotidine. The assay result of synthetic mixture was found to be...
Journal of Liquid Chromatography & Related Technologies, 2013
Accurate, sensitive, and precise high-performance thin-layer chromatographic (HPTLC) method was developed and validated for the determination of Methocarbamol (ME) and its related substance (guaifenesin (GU)) in two ternary mixtures with ibuprofen (IB) and diclofenac potassium. The method depends on separation and quantification of the studied drugs on TLC silica gel 60 F 254 plates using ethyl acetate-acetone-triethylamine-formic acid (62:35:6:0.3, by volume) as the developing system followed by densitometric measurement of the bands at 222 nm for the first mixture containing methocarbmol, IB, and GU and at 278 nm for the second mixture containing methocarbmol, diclofenac potassium, and GU. The proposed methods were successfully applied for the determination of ME, IB, and diclofenac potassium in the presence of ME-related substance (GU) either in bulk powder or in their pharmaceutical formulations.
Spectrophotometric Determination of Ibuprofen in Visible Region of Spectrum
2013
Ibuprofen reacts with α – naphthylamine and sodium nitrite to give orange color having maximum absorbance at 460 nm. The reaction is specific for Ibuprofen and provides a basis for its spectrophotomertic determination. The color reaction obeys Beer’s law from 0.1 mg to 10 mg/10 ml of Ibuprofen. The relative standard deviation is 1.25%. The quantitative assessments of tolerable amount of other drugs have also been studied.
Method Development for Visible Spectrophotometric Analysis of Ibuprofen in Pharmaceuticals
Ibuprofen is a prominent member of the group of non-steroidal anti-inflammatory drugs (NSAIDs), with good antiinflammatory action, a very effective analgesic, with increased antipyretic effect. The aim of this research was to exactly quantify pure Ibuprofen content in tablets of a pharmaceutical, by a spectrophotometric analysis method in the Visible range. Ibuprofen was quantitatively converted to a bright orange dye with a yellowish shade, by a color reaction with alphanaphthylamine in the presence of sodium nitrite, in an absolute ethanol medium. Following the analysis, it was found 397.952 milligrams of pure Ibuprofen content / film-coated tablet of the pharmaceutical product. This value was very close to Ibuprofen content declared by the pharmaceutical manufacturer (400 milligrams), with a mean deviation of only 0.512 percent from the officially declared amount of active substance. The value found fits perfectly within the normal limits provided by the European and International Pharmacopoeias standards, taken over by the Romanian Pharmacopoeia, 10th Edition. The spectrophotometric analysis method was then successfully subjected to statistical analysis.
Simple and selective extractive spectrophotometric methods for the determination of quetiapine hemifumarate (QF) were developed and validated. The methods were based on the formation of yellow ion-pair complexes between QF and acidic dyes namely bromcresol purple (BCP) and bromcresol green (BCG) at room temperature in phosphate buffer (pH 3.0). The formed complexes were extracted with chloroform and the absorbances were measured at 406.5 nm for BCP and at 416 nm for BCG complexes. The compositions of the ion-pairs were found as 1:1 by mole-ratio method. The reaction conditions such as concentration, pH, color formation time, temperature and chromogen stability were optimized. Good linear relationship was obtained between the absorbance and the concentration of QF in the range of 0.5 -20 Hg/mL for both BCP and BCG (r > 0.9974). LOD values were found as 0.12 and 0.16 \ig/mLfor BCP and BCG complexes, respectively. Intra-day precisions were found less than 1 % in the methods. The developed methods were applied successfully to the determination of QF in tablets marketed in Turkey.
International journal of Pharmacy and Pharmaceutical Sciences, 2014
Objective: To develop a simple and cheap UV spectrophotometric method for the simultaneous quantitative estimation of Mebeverine hydrochloride (135mg) and Chlordiazepoxide (5mg) in MEVA C Capsules and validate as per ICH guidelines. Methods: The optimized method uses a diluent 100% Triethylammonium phosphate buffer (pH 3.0) for the estimation of assay of Mebeverine hydrochloride and Chlordiazepoxide in Capsules which are analyzed at a detection wavelength of 260nm. Results: The developed method exhibited linearity in the range of 10-40μg/ml for Mebeverine hydrochloride and 2.5-10μg/ml for Chlordiazepoxide. The precision for Mebeverine hydrochloride and Chlordiazepoxide is exemplified by relative standard deviation of 0.499% and 1.75 respectively. Percentage Mean recovery for Mebeverine hydrochloride and Chlordiazepoxide were found to be in the range of 98-102, during accuracy studies. The limit of detection (LOD) for Mebeverine hydrochloride and Chlordiazepoxide were found to be 321ng/ml and 3.7ng/ml respectively, while limit of quantitiation (LOQ) for Mebeverine hydrochloride and Chlordiazepoxide were found to be 973ng/ml and 11.2ng/ml respectively. Conclusion: A simple and a cheap UV spectrophotometric method was developed and validated for the simultaneous quantitative estimation of Mebeverine hydrochloride and Chlordiazepoxide in capsules as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries.
Journal of Pharmaceutical and Biomedical Visible Spectrophotometric Determination of
2013
Paracetamol (acetaminophen) is a widely used over-the-counter analgesic, antipyretic and a mild anti inflammatory drug. In several developing countries some of pharmaceutical industries sale fake, counterfeit and substandard drugs which affect the health of people. The present study investigates the comparison for the quantitative determination of various brands of paracetamol tablet using UV-Visible spectrophotometric, potentiometric and trimetric methods. Four brands (Pacimol, Paracip, Parazest, and Crocin) of paracetamol tablets having 500 mg strength were purchased from various pharmacy shops within Pari chowk and Jagat market in Greater Noida, India. Weight variation test was performed before the assay of paracetamol samples. The result of tablets weight variation (Mean ± S.D) of Crocin, Parazest, Paracip and Pacimol brand was 0.66 ± 0.014, 0.64 ± 0.010, 0.58 ± 0.007 and 0.55 ± 0.009 respectively. All brands showed different mean weight which indicates the use of different excipients in the different brands. The ranges of the amount of paracetamol content (g/tab) for paracetamol samples analyzed using UV-Visible spectrophotometric, potentiometric and trimetric methods were from 0.49195-0.52010, 0.48300-0.52100 and 0.48106-0.50110 respectively. The results indicated that all four brands of paracetamol tablets have sufficient quantity and amount is approximately similar to the company's recommended or claimed value. Similarly, the ranges of percentage content (assay) of the analyzed samples using UV-Visible spectrophotometric, potentiometric and trimetric methods were from 98.69-104.20%, 96.60-104.20%, and 96.21-100.22% respectively. The assay results indicated that variation among all brands and this may show that different manufacturer formulates the different brands are under the IP specification. However, all of the brands of the tablets under the study were complied with the IP specification and passed for weight variation test and assay conducted on it. Hence, the drug control authority of the government should be continuously monitored the safety, quality, and efficacy of paracetamol tablet through post marketing surveillance practices, and the proper internal quality control of the pharmaceutical companies need to take further necessary steps to ensure the continuity in the establishment of the product quantity and quality.
Determination of binary mixture of ibuprofen and famotidine by different spectrophotometric methods
European Journal of Chemistry, 2016
Four simple and specific spectrophotometric methods were developed and validated for the simultaneous determination of binary mixture of ibuprofen and famotidine, using unified regression equation. The proposed spectrophotometric procedures including, derivative ratio, ratio subtraction, dual wavelength and mean centering of ratio spectra do not require any separation steps. Accuracy, precision and linearity ranges of the proposed methods were determined and the specificity was assessed by analysing synthetic mixtures of both drugs. The methods were applied to a pharmaceutical formulation and the results obtained showed that there is no significant difference between the proposed methods and the reported one regarding both accuracy and precision.