Effect of N-dodecyl-N,N-dimethylamine N-oxide on unilamellar liposomes (original) (raw)

The interaction of N-dodecyl-N,N-dimethylamine N-oxide (C12NO) with unilamellar liposomes composed of EYPC was systematically investigated using turbidimetric and fluorescence spectroscopic methods. A three-stage model of surfactant-membrane interaction was employed to describe the phase transformations occurring with varying surfactant concentrations. Initial binding of surfactant monomers leads to membrane perturbation, progressively resulting in increased permeability as higher concentrations induce the formation of mixed micelles. Observations suggest that significant cytotoxic effects and structural modifications to the liposomes arise under specific C12NO concentrations, emphasizing the importance of using complementary analytical techniques for understanding surfactant behavior.