ANTIMICROBIAL ACTIVITY OF SESBANIA GRANDIFLORA (L (original) (raw)
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Aim: The antimicrobial effect of aqueous and ethanolic extracts from leaves and stem of Sesbania grandiflora (L) against four clinical isolates, namely, Staphylococcus aureus, Escherichia coli, Salmonella enteric and Pseudomonas aeruginosa is the major aim of this research work. Methodology: Using well agar diffusion method, the sensitivity patterns of both crude and different fractional concentrations of ethanolic and aqueous leaves and stem extracts of S. grandiflora were Original Research Article Abubakar et al.; BMRJ, 8(2): 424-433, 2015; Article no.BMRJ.2015.135 425 tested. Cold maceration method of extraction was used to extract the plant materials. Fifty grammes of the ground dry sample was placed in 500 cm 3 of distilled water and 95% ethanol for 72 hours. After which the supernatant was decanted off, then evaporated to dryness in (Buchi, water bath UK) at 40°C and the crude extract collected was weighed and stored by refrigeration at temperature of 4°C for further uses. Results: The extracts showed significantly varying inhibitory activities against the test organisms as evidenced by the zone diameter of inhibition. The aqueous and ethanolic leaves extract were found to be active against S. aureus in all the concentrations with zone diameter of inhibition ranging between 9.0±00 mm to 24.0±0.21 mm while the ethanolic stem extract was found to be active against S. enterica with zone diameter of inhibition ranging between 11±0.30 mm to 23±0.21 mm. The fractions obtained from the column chromatography were found to be active against S. aureus, with zone diameter of inhibition ranging between 8±0.00 mm to 21±0.00 mm. Ampicillin and Chloramphenicol were used as positive control. Both the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts ranged between 125 mg/ml to 31.25 mg/ml and 62.5 mg/ml to 15.63 mg/ml respectively. The phytochemical screening divulged the presence of alkaloids, flavonoids, tannins, steroids and terpenoids in both the plant extracts, pholobatannins were absent in aqueous stem extract while anthraquinonnes were absent in both aqueous and ethanolic leaves and stem extracts respectively. Conclusion: S. grandiflora may represent new sources of antimicrobials with stable, biologically active components that can establish a scientific base for the use of plants in modern medicine. This research also justified the scientific basis for the use of the crude leaves extract in the traditional system of medicine for the treatment of diseases especially in treatment of typhoid fever and gastrointestinal infections.
The aim of the present study is to evaluate the antibacterial activity of ethanolic and aqueous leaf extract of Sesbania grandiflora Linn on selected bacterial species. Sesbania grandiflora (Linn) is a medicinal herb belongs to the family Leguminosae .The various parts of the plant like roots, bark, leaves, flower and fruit are known to possess different pharmacological properties. The leaf extract of Sesbania possess good anticonvulsant and anxiolytic activity. The leaves of Sesbania is also found to possess hepatoprotective and potent anti oxidant and anti urolithiatic activity .The Ethanolic and Aqueous extract of leaves were screened for the antimicrobial activity by agar well diffusion method. The test organisms used were Staphylococcus aureus, E.coli, Klebsiella pneumoniae, Psuedomonas aeruginosa and Bacillus subtilus. The results obtained from the study showed that the ethanolic extract has significant antibacterial activity on Staphylococcus aureus and E.coli compared with standards.
Biological activities and antibacterial biomarker of Sesbania grandiflora bark extract
Drug discoveries & therapeutics, 2017
In the present study, the fractionated extracts of Sesbania grandiflora bark were prepared and evaluated for their biological activities. The ethyl acetate fractionate (EAF) showed high antioxidant activity along with free radical scavenging and reducing mechanisms. The free radical scavenging antioxidant activity of EAF was 69.3 ± 3.6% where its Trolox equivalent antioxidant capacity was 13.6 ± 0.7 mM/mg. EAF exhibited the reducing power equivalent to ferrous sulfate at 152 ± 2 mM/mg and equivalent to gallic acid at 1.05 ± 0.01 mM/mg. In addition, EAF presented high potential on inhibition of bacterial growth with the minimum bactericidal concentration less than 1 mg/mL. Further isolation of EAF using normal-phase open column of silica gel 60, showed that the fractions eluted with the mixture of chloroform and methanol at the ratios of 4:1, 3:2, and 2:3 possessed antibacterial activity. The recovery activity of total different active fractions was 5% EAF, 20 times less than that of...
International Journal of Pharma and Bio Sciences, 2019
Sesbania sesban, belonging to family Leguminosae, is a well-recognized medicinal plant. It is widely distributed in our country. Sesbania sesban is an erect, branched small tree up to 6 m tall with soft wood and paripinnate leaves. Flowers are yellow with brown streaks on the corolla. Fruits are sub cylindrical and shortly beaked. Seeds are green or brown and usually mottled. The plant is used as carminative, antihelmintic, astringent, anti-inflammatory, antimicrobial, antifertility, demulcent and purgative. It is also given as a medicine against fever andulcers. The medicinal value of plants depends up on the chemical compounds that produce in in the human body during a specific reaction. chemical substances responsible for such reactions are called secondary metabolites. The most important of these bioactive compounds are alkaloids, terpenoids, steroids, flavonoids, tannins and phenolic compounds. By keeping these properties of bioactive compounds of Sesbania sesban in view the present investigation was undertaken to screen phytochemical constituents. Preliminary phytochemical screening of leaf in three different extracts (ethanol, methanol and aqueous extract) revealed the presence of carbohydrates, tannins, saponins, glycosides, steroids, flavonoids, phenols, terpenoids, alkaloids and glycosides which indicated antimicrobial activity of the Sesbania sesban leaves. The other objective was to investigate antimicrobial activity of three solvent extracts against gram negative pathogenic bacteria viz, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. Among the three extracts, ethanol showed the maximum activity. Zone of inhibition caused by ethanol against Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae at (50µl, 100µl, 150µl) is 25%, 24.5% and 18.8% higher, respectively than caused by methanol and aqueous extracts of Sesbania leaves. Ethanol extract of Sesbanialeaves potentially act as antimicrobial agent.
Anti-bacterial and anti-fungal evaluation of bark extracts of Sesbania grandiflora L
International journal of health sciences
The aim of the present study is to evaluate the antibacterial and antifungal activity of bark extracts of Sesbania grandiflora L. The antibacterial screening was done by Paper disc method and antifungal screening by Disc Diffusion method using pet ether, chloroform, aqueous and methanolic extracts. The results showed strong potential of both the activity of methanolic extracts as compared with that of the other extract.
ANTIMICROBIAL ACTIVITY OF SESBANIA GENUS PLANTS
ijetrm journal, 2021
Background: Sesbania genus plants are traditionally being use for medicinal purposes in India. Present study was designed to check in-vitro efficacy of these plants against selected bacterial and fungal strains. Methodology: Hydroalcohol extracts of these plants were used for antimicrobial screening. Antibacterial activity was tested against four pathogenic bacterial strains i.e., Escherichia coli and Staphylococcus aureus while antifungal activity was tested against two fungal strains i.e., Candida albicans and Aspergillus niger Results: Hydroalcohol extracts of Sesbania genus plants showed high inhibitory activities against E. coli and S. aureus. As antifungal activities, hydroalcohol extracts shows excellent activity against Aspergillus niger and Candida albicans. Conclusions: These findings provide scientific evidence of traditional use of medicinal plants and indicate the potential of these plants for the development of antimicrobial agents.
Phytochemical study on Sesbania grandiflora
2013
Sesbania grandiflora Linn. (Family: Fabaceae) is widespread distributed in West Bengal, Assam, Karnataka and North-Eastern parts. The present study intended with various phytochemical screening and toxicity studies on Sesbania grandiflora. The present study was done with the help of soxhlet extraction, Kirby Bauer well diffusion assay, Minimum Inhibitory concentration assay (MIC) and Colony forming unit (CFU). Preliminary phytochemical evaluation of the aqueous extracts revealed that presence of carbohydrate, proteins, flavonoids, alkaloids, tannins and glycosides. As per the antimicrobial investigations in the presence study the CLP (Crude Leaf Powder) of Sesbania grandiflora exhibited low level antibacterial activity but the NPL (Nanosized leaf powder) exhibited highest level of antibacterial activity.
Antibacterial activities of Sesbania grandiflora extracts
Drug Discoveries & Therapeutics, 2011
Silkworm has been proposed recently as an animal model for the study of therapeutic effects of drug candidates. Little is known regarding the factors influencing the physiology of the silkworm, and this might become the major effect of the therapeutic result interpretation. In the present study, the effect of a diet of mulberry leaves as compared to an artificial diet on Thai silkworm growth development as well as the antibiotic therapeutic responses of bacterial infected silkworms is explored. The silkworms fed with mulberry leaves as well as those with artificial diets were weighed and the numbers of survival were counted. The mulberry leavesfed silkworms (MFS) showed faster growth rate (0.2 g body weight day −1) than the artificial diet-fed silkworms (AFS). The MFS exhibited bigger body size and greater body weight than the AFS. It was found that the median lethal dose (LD 50) of Staphylococcus aureus cells needed to kill 1-g body weight silkworms was 10 8 cells ml −1 for both MFS and AFS. However, the median effective dose (ED 50) of the antibiotics (ampicillin, ceftriaxone, cephalexin, and gentamicin) for AFS was two times higher than those for MFS. The results indicate that some elements in mulberry leaf affect the therapeutic effect of the antibiotics in the silkworm model. This study suggests that high attention on silkworm diet should be paid to the research relevant to drug therapeutic evaluation using silkworms as an animal model.
Medicinal plants have been considered as important therapeutic aid for alleviating ailments of man kind. In spite of tremendous development in the field of allopathy. Medicinal plants and their derivatives still remain one of the major roles in medicinal therapy. The world's health organization also has recognized the important of traditional medicine and has been active in creating strategies guidelines and standard for botanical medicines. Sesbania grandiflora Linn belongs to the family Leguminosae and has been widely used in Ayurveda. Agati is a widely available, fast growing plant, generally popular for animal fodder. The plants parts used are Root, Bark, Leaf, Flower and Fruit. Sesbania grandiflora known as agati, syn. Aeschynomene grandiflora or hummingbird tree/scarlet wisteria is a small tree in the genus Sesbania, commonly it is known as caturay, katurai, Chamorro, corkwood tree, scarlet wisteria, sesban, vegetable hummingbird in English, agathi, in Tamil, hadga in hindi. In Ayurveda the plant has been used for the treatment of head ache, for fever 1,2 as tonic, in cataract and as astringent. The leaves of the plant have been reported to have anxiolytic 3-9 and anticonvulsant effect while the flowers have been reported to have antimicrobial activity 10 , hypolipidemic, antiulcer 11 and anti-inflammatory effects. In view of the above considerations the present study was designed to investigate the antibacterial activity of ethanolic root extract of Sesbania grandiflora.
Medicinal and Therapeutic Value of Sesbania grandiflora
2019
There are around 60 global species belonging to the genus Sesbania which are commonly found to be grown in Africa, Australia, and Asia. The leaves of Sesbania grandiflora have been used in local traditional medicine since ancient times. Major chemical constituents are alkaloids, flavonoids, glycosides, tannin, anthraquinone, steroid, pholobatannins, and terpenoids. Isovestitol, medicarpin, sativan (isoflavonoids) and betulinic acid (tannin substance) are the major constituents responsible for antibacterial and antifungal, antioxidant, anti-urolithiatic, anticonvulsant and anxiolytic, and hepatoprotective properties. Also, the plant extract contains alkaloids, phenolics, tannins, triterpenoids, and sterols. All parts of S. grandiflora are used in traditional medicine and phytochemical investigations have been conducted on extracts of the leaves, seeds and roots of S. grandiflora to provide scientific validation of its properties. The juice of the leaves of S. grandiflora has been rep...