Enhanced Removal of a Pesticides Mixture by Single Cultures and Consortia of Free and Immobilized Streptomyces Strains (original) (raw)
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Environmental science and pollution research international, 2017
The current study aimed to evaluate the removal of a pesticide mixture composed of the insecticides chlorpyrifos (CP) and diazinon (DZ) from liquid medium, soil and a biobed biomixture by a Streptomyces mixed culture. Liquid medium contaminated with 100 mg L(-1) CP plus DZ was inoculated with the Streptomyces mixed culture. Results indicated that microorganisms increased their biomass and that the inoculum was viable. The inoculum was able to remove the pesticide mixture with a removal rate of 0.036 and 0.015 h(-1) and a half-life of 19 and 46 h(-1) for CP and DZ, respectively. The sterilized soil and biobed biomixture inoculated with the mixed culture showed that Streptomyces was able to colonize the substrates, exhibiting an increase in population determined by quantitative polymerase chain reaction (q-PCR), enzymatic activity dehydrogenase (DHA) and acid phosphatase (APP). In both the soil and biomixture, limited CP removal was observed (6-14%), while DZ exhibited a removal rate ...
Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains
International Journal of Environmental Science and Technology, 2014
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Growth and Adaptation of Four Streptomyces Isolates in the Media Containing Propoxur
Actinomycetes growth in the media containing carbamates is an interesting subject associated to its adaptation and metabolism behavior. In this work, four isolates of Streptomyces spp. had been approved to grow and demean propoxur, a carbamates pesticide commonly uses to control pest insect. The Streptomyces spp. cultures were incubated for seven days in the media containing propoxur, and incubated in the rotary (50 rpm) shaker-bath at 35 0 C. Microbial population calculated based on culture dry weight throughout separating supernatant and biomass sedimentation in the media with centrifugation work. Propoxur degradation evaluated during the isolates refined in yeast extract media containing starch (YSB) and without starch (YB), then the propoxur (0, 200, 600, 1200, and 1800 ppm) were added to the media. After a period of incubation, propoxur content in the media determined with hydrolysis to become 2-isopropoxyphenol method, and with diazotized 3-aminopyridine processes turn into az...
Isolation of four aquatic streptomycetes strains capable of growth on organochlorine pesticides
Bioresource Technology, 2003
Ninety-three wild-type isolates identified as actinomycetes were tested against 11 organochlorine pesticides (OPs): aldrin, chlordane, DDD, DDE, DDT, dieldrin, heptachlor, and heptachlor epoxides, lindane, and methoxychlor. Qualitative screening agar assays displayed 62-78% tolerance of strains to OPs. Four strains designed M4, M7, M9 and M15 were selected based on multi-OPtolerance, and identified as members of the streptomycetes group. Different growth profiles were observed in cultures of the four selected streptomycetes cultured in synthetic medium containing 5-50 lg l À1 aldrin or chlordane or lindane. Increase of aldrin removal by the selected microorganisms was concomitant with the 4.8-36.0 lg l À1 pesticide concentration range. After 72 h of streptomycete M7 growth in synthetic medium containing 48.0 lg l À1 aldrin, the remaining OP concentration in the supernatant was approximately 10% of the initial concentration. Also, in stationary growth phase less than 2.5 lg l À1 aldrin residual concentration was detected in the medium.
Pesticide removal by a mixed culture of bacteria and white-rot fungi
Journal of the Taiwan Institute of Chemical Engineers, 2012
Combining activated sludge cultures with microbes harboring specific degradation pathways could constitute a relevant process for the removal of toxic and recalcitrant organic substances from wastewater. Enhanced removal of three widely used recalcitrant pesticides from their liquid mixture was demonstrated by implementing a non-acclimated mixed culture of bacteria and white rot fungus. During an incubation period of 14 days, the mixed fungusbacteria culture achieved 47, 98, and 62% removal of aldicarb, atrazine and alachlor from the liquid phase, respectively. This compared favorably to batches containing only nonacclimated fungus or previously published removal rates with non-acclimated bacterial cultures. Biosorption along with biodegradation was responsible for the removal of the pesticides from the liquid phase. Potential application modes of the studied biodegradation process were also discussed.
Applied Microbiology and Biotechnology, 1993
We investigated the degradation of 2,4,6trichlorophenol (2,4,6-TCP) by cells of Streptomyces rochei 303 immobilized on various carriers. Polycaproamide fibre was chosen as the optimal carrier for immobilization. The cells immobilized on this carrier degraded high-concentrations of individual chlorophenols and their mixtures: from mono-to pentachlorophenol including the most persistent meta-substituted derivatives. During continuous fermentation in a column with continuous substrate and air flow at a maximal degraded concentration of 2,4,6-TCP of 1 g/1 and the specific flow rate of 0.08 h-1, the efficiency of degradation was 720 mg 2,4,6-TCP/day (36 mg 2,4,6-TCP/day per gram of carrier). The above system of immobilized cells was operated continuously without any loss of activity for 2.5 months, the amount of degraded 2,4,6-TCP being 54 g. At a lower concentration of the reagent (150 mg/1) the system was operated without any decrease in its degradability and without any additional carbon source for 11 months.
International Biodeterioration & Biodegradation, 2014
Streptomyces strains isolated from pesticide-contaminated sediments of Santiago del Estero, Argentina were able to grow in the presence of 1.66 mg L À1 of methoxychlor (MTX). Streptomyces sp. A14 showed the best growth in the presence of MTX in culture medium at 30 C and pH 7. When soil microcosms were contaminated with MTX and inoculated with Streptomyces sp. A14, a decrease in MTX was detected in both concentrations assayed (8.33and 16.60 mg kg À1 ). This actinobacterium was able to remove the pesticide, reaching its maximum removal percentages (40% and 76%) after 28 days of incubation. Methoxychlor was gradually converted into 1,1-dichloro-2,2-bis(4-methoxyphenyl)ethane, 1,1-dichloro-2,2-bis(4-methoxyphenyl)ethylene, 1-chloro-2,2-bis(4-methoxyphenyl)ethane, and 2,2-bis(4hidroxyphenyl)acetonitrile, indicating that MTX is dominantly degraded by dechlorination, dehydrogenation and CN-replacement, resulting in the production of several major degradation products. This is an interesting proposal as an alternative method for soil cleanup. This is the first report of methoxychlor degradation using a native Streptomyces strain under aerobic conditions.
INACTIVATION OF STREPTOMYCES GRISEUS BY COMMON WATER TREATMENT DISINFECTANTS
Actinomycetes are known to be odor -causing bacteria. The present project used Streptomyces griseous subsps. Griseous ATCC ® 3343 for different inactivation procedures and to spike them into an existing pipe -loop apparatus. Streptomycetes are members of the Actinomycetales family. The objectives define in this project were to obtain inactivation rates for streptomyces using different disinfectants, compare Actinomycetes inactivation with literature values and other bacteria and finally to determine the fate of Actinomycetes spiked into a laboratory pipe-loop PVC apparatus with subsequent chlorination. Several studies have demonstrated this subspecies produced very strong and foul odors. Among the metabolites produced by Streptomycetes are Geosmin and 2-Methylisoborneol (MIB). The maximum geosmin concentration achieved during this project was 6.63 ng/L, while the maximum MIB concentration was 125.23 ng/L. During the length of this research it was observed that stressful conditions altered physical properties such as shape, size and color of the colonies. Odorproduction was also affected by media variations and their insertion into the polyvinyl chloride (PVC) pipe -loop. Actinomycetes were found on several samples sites within Arizona State University Main Campus and on a canal wall prior to the Deer Valley Water Treatment Plant.
The ability of Actinomycetes strains to degrade pollutant matters and to reduce or eliminate pathogens microorganisms from domestic wastewater of an industrial site (oilfield of Tsimiroro-Madagascar) at the laboratory scale is demonstrated in the present work. Two most active Actinomycetes isolates (Streptomyces albidoflavus strain DSM 40455T and Streptomyces antibioticus strain NBRC 12838T) against test-pathogens were selected for the purification treatment. The analysis of physico-chemical (COD, BOD, pH, conductivity, color, TDS, nitrite, nitrate, phosphate and chloride rates) and microbiological parameters (sulphite reducing anaerobe, fecal coliforms, fecal Streptococcus and Escherichia coli rates) allowed to evaluate the quality of the wastewater. Physico-chemical results revealed that purified water is qualitatively improved view that 60.86% of TDS, 71.61% of its color, 25.55% of its chloride rate, 45.32% of its nitrate rate, 99.9% of its nitrite rate, 26.25% of its phosphate rate, 46.53% of its initial COD and 58.11% of its BOD were eliminated at the end of the treatment. Only, the conductivity increased compared with the guideline values for all treatment. The process improved also microbiological quality of the wastewater with total elimination of fecal Streptococcus and diminution of fecal coliforms, sulphite reducing anaerobe and Escherichia coli concentrations. The experiment proved that biological treatment using Actinomycetes strains is a promising, less expensive and simple technology for wastewater recycling ensuring thus their reuse for other activities. Cite This Article: Andriambeloson Onja, Rabenirina Harilanto, Rajoelisoa Andriamalala, Ramaroson Luciano, and Rasolomampianina Rado, " Purifying Potential of Streptomyces albidoflavus Strain DSM 40455T and Streptomyces antibioticus Strain NBRC 12838T in Wastewater Treatment.