Role of gallium and silver from phosphate-based glasses on in vitro dual species oral biofilm models of Porphyromonas gingivalis and Streptococcus gordonii (original) (raw)
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Bio-medical materials and engineering, 2014
Gallium and silver incorporated phosphate-based glasses were evaluated for antibacterial effect on the growth of Pseudomonas aeruginosa, which is a leading cause of opportunistic infections. The glasses were produced by conventional melt quenching methods at 1100°C for 1 h. Glass degradation studies were conducted by weight loss method. Disc diffusion assay and cell viability assay displayed statistically significant (p ≤ 0.0005) effect on P. aeruginosa growth which increased with decreasing calcium content in the glasses. The gallium ion release rates (1.83, 0.69 and 0.48 ppm·h(-1)) and silver ion release rates (2.97, 2.84 and 2.47 ppm·h(-1)) were found to account for this variation. Constant depth film fermentor was used to evaluate the anti-biofilm properties of the glasses. Both gallium and silver in the glass contributed to biofilm growth inhibitory effect on P. aeruginosa (up to 2.68 reduction in log 10 values of the viable counts compared with controls). The glasses were foun...
Dental Materials, 2008
Objectives. The purpose of this study was to evaluate the in vitro antibacterial and biological activity of silver-incorporated bioactive glass system SiO 2-CaO-P 2 O 5-Ag 2 O (AgBG). The bacteriostatic and bactericidal properties of this new quaternary glass system along with the ternary sol-gel glass system SiO 2-CaO-P 2 O 5 (BG) have been studied using Escherichia coli as a test microorganism. The AGBG system thus appears to be a promising material for dental applications, since similar effects might be produced on a film of bacteria and mucous that grows on the teeth. Methods. The SiO 2-CaO-P 2 O 5-Ag 2 O and SiO 2-CaO-P 2 O 5 glass systems were synthesized by the sol-gel technique and characterized for their physicho-chemical properties. The antibacterial activity and biological properties were evaluated by determining the minimum inhibitory concentrations (MICs). Release of Ag + into the culture medium was measured by inductively coupled plasma (ICP) analysis. Results. The in vitro antibacterial action of the SiO 2-CaO-P 2 O 5-Ag 2 O was compared with that of its ternary counterpart glass system. The concentrations of Ag-bioglass, in the range of 0.02-0.20 mg of Ag-bioglass per millilitre of culture medium, were found to inhibit the growth of these bacteria. The Ag-bioglass not only acts bacteriostatically but it also elicited a rapid bactericidal action. A complete bactericidal effect was elicited in the early stages of the incubation at Ag-bioglass concentration of 20 mg/ml and the ternary glass system had no effect on bacterial growth or viability. The antibacterial action of Ag-bioglass was exclusively attributed to the leaching of Ag + ions from the glass matrix. Significance. One of the major advantages of incorporating silver ions into a gel glass system is that the porous glass matrix can allow for controlled sustained delivery of the antibacterial agent to dental material, used even under anaerobic conditions such as deep in the periodontal pocket. This glass system also provides long-term action required for systems which are constantly at risk of microbial contamination.
Effect of Silver-Doped Phosphate-Based Glasses on Bacterial Biofilm Growth
Applied and Environmental Microbiology, 2008
Silver-containing phosphate-based glasses were found to reduce the growth of Pseudomonas aeruginosa and Staphylococcus aureus biofilms, which are leading causes of nosocomial infections. The rates of glass degradation (1.27 to 1.41 μg·mm −2 ·h −1 ) and the corresponding silver release were found to account for the variation in biofilm growth inhibitory effect.
Potential use of gallium-doped phosphate-based glass material for periodontitis treatment
Journal of Biomaterials Applications, 2015
This study aimed at evaluating the potential effect of gallium-incorporated phosphate-based glasses towards periodontitis-associated bacteria, Porphyromonas gingivalis, and matrix metalloproteinase-13. Periodontitis describes a group of inflammatory diseases of the gingiva and supporting structures of the periodontium. They are initiated by the accumulation of plaque bacteria, such as the putative periodontal pathogen Porphyromonas gingivalis, but the host immune response such as elevated matrix metalloproteinases are the major contributing factor for destruction of periodontal tissues. Antibacterial assays of gallium-incorporated phosphate-based glasses were conducted on Porphyromonas gingivalis ATCC 33277 using disc diffusion assay on fastidious anaerobe agar and liquid broth assay in a modified tryptic soy broth. In vitro study investigated the effect of gallium on purified recombinant human matrix metalloproteinase-13 activity using matrix metalloproteinase assay kit. In vivo bi...
Antimicrobial Agents and Chemotherapy, 2007
Staphylococcus aureus can cause a range of diseases, such as osteomyelitis, as well as colonize implanted medical devices. In most instances the organism forms biofilms that not only are resistant to the body's defense mechanisms but also display decreased susceptibilities to antibiotics. In the present study, we have examined the effect of increasing silver contents in phosphate-based glasses to prevent the formation of S. aureus biofilms. Silver was found to be an effective bactericidal agent against S. aureus biofilms, and the rate of silver ion release (0.42 to 1.22 g ⅐ mm ؊2 ⅐ h ؊1 ) from phosphate-based glass was found to account for the variation in its bactericidal effect. Analysis of biofilms by confocal microscopy indicated that they consisted of an upper layer of viable bacteria together with a layer (ϳ20 m) of nonviable cells on the glass surface. Our results showed that regardless of the silver contents in these glasses (10, 15, or 20 mol%) the silver exists in its ؉1 oxidation state, which is known to be a highly effective bactericidal agent compared to that of silver in other oxidation states (؉2 or ؉3). Analysis of the glasses by 31 P nuclear magnetic resonance imaging and high-energy X-ray diffraction showed that it is the structural rearrangement of the phosphate network that is responsible for the variation in silver ion release and the associated bactericidal effectiveness. Thus, an understanding of the glass structure is important in interpreting the in vitro data and also has important clinical implications for the potential use of the phosphate-based glasses in orthopedic applications to deliver silver ions to combat S. aureus biofilm infections.
Role of Bio-synthesized silveR in ContRolling PeRiodontoPathiC BaCteRia
Egyptian dental journal
The microbial etiology of gingivitis and periodontitis provides the rationale for use of adjunctive antimicrobial agents in the prevention and treatment of periodontal diseases. objectives: To assess the possible inhibiting effect of bio-synthesized nano-silver (Bio/Ag NPs) on a selected suspected periodontopathic bacterium (Actinomyces oris). Methodology: subgingival plaque samples were obtained out of 35 systemic-free patients with chronic periodontitis following the removal of supragingival plaque and calculus. The collected samples were immediately transferred for Actinomyces oris lab culturing. Besides, isolation and identification of Bio/Ag NPs producing bacteria, the sequence analysis used for the obtained DNA nucleotides sequence was identified by PCR using 16S rRNA gene revealed that the examined strain is P. aeruginosa (GU393321). Nano silver powder was examined by x-ray diffraction and scanning electron microscope (SEM) for characterizing its composition and particle size respectively. The activity of the bio-synthesized Bio/Ag NPs (0.1, 0.3 and 0.5 ppm) was examined against the suspected bacterium using the agar diffusion method. Three doses, 100 µl each, of 0.1, 0.3 and 0.5 ppm bio-nano-silver solutions were dispensed into standardized agar wells before planting the bugs using sterilized cotton sticks. The plates were incubated for 24 h and then assessed for the presence of bacterial growth inhibition zones. Results: The lowest concentrations of bio-nano-silver (0.1 and 0.3 ppm) failed to inhibit the growth of the selected bacterium. However the higher concentration (0.5 ppm) showed a significant bacterial inhibition in 2cm in diameter zone compared with three different types of routinely used antibiotics.
PloS one, 2012
This paper reports the effect of soda-lime-glass-nAg coating on the viability of an in vitro biofilm of Streptococcus oralis. Three strains (ATCC 35037 and two clinical isolates from periodontitis patients) were grown on coated with glass, glass containing silver nanoparticles, and uncoated titanium alloy disks. Two different methods were used to quantify biofilm formation abilities: crystal violet staining and determination of viable counts. The influence of the surface morphology on the cell attachment was studied. The surface morphology was characterized by scanning electron microscopy (SEM) and using a profilometer. SEM was also used to study the formation and the development of biofilm on the coated and uncoated disks. At least a .99.7% inocula reduction of biofilm respect to titanium disks and also to glass coated disks was observed in the glass-nAg coated disks for all the studied strains. A quantitative evaluation of the release of silver was conducted in vitro to test whether and to what extend the biocidal agent (silver) could leach from the coating. These findings suggest that the biofilm formation of S. oralis strains is highly inhibited by the glass-nAg and may be useful for materials which require durable antibacterial effect on their surfaces, as it is the case of dental implants.
Anti-biofilm activity of silver nanoparticle-containing glass ionomer cements
Dental Materials, 2020
Objective. To develop a silver nanoparticle (AgNP) formulation for incorporation into glass ionomer cements (GICs) which minimises biofilm growth on restoration surfaces. Methods. GICs, Fuji IX, Ketac Molar, and Riva Selfcure were modified with 6, 10 and 24 g per GIC capsule of ␣-lipoic acid-capped AgNPs. Monoculture biofilms of Streptococcus mutans were cultured (72 h) on GIC specimens (n = 3) and biofilm accumulation was quantified using a viability stain with confocal laser scanning microscopy. Compression strength and flexural strength (CS & FS) were measured according to ISO 9917-1:2007 (n = 8, n = 25). GIC colour was measured at 0, 1, and 14 days following AgNP incorporation using a digital spectrophotometer. Silver release from AgNP-modified GIC specimens was monitored at 1, 3, 7 and 14 days using inductively coupled plasma-mass spectrometry. Results. AgNP-modified Fuji IX demonstrated the greatest reduction in biofilm accumulation, with 10 g Ag/capsule inhibiting biofilm formation by 99%. Ketac Molar and Riva Selfcure required 24 g Ag/capsule to achieve 78% biofilm reduction. AgNP-modified GICs demonstrated significantly higher CS and FS than sintered silver-containing GICs, and possessed equivalent or higher strength values when compared to unmodified GICs. The colour shades of AgNP-modified GICs were more comparable to VITA shades of non-modified GICs than were sintered silver-containing GICs. The silver (≥99.6%) remained within the GIC for at least two weeks following incorporation. Significance. AgNP-modified GICs exhibited significant antibiofilm activity and retained mechanical properties equivalent or superior to non-modified GICs. AgNP-modified GICs could reduce bacterial colonisation on and around restorations thereby reducing restoration failure caused by secondary caries.
Efficacy of a silver colloidal gel against selected oral bacteria in vitro
F1000Research
Background: It is necessary to develop new strategies to protect against bacteria such as Streptococcus mutans, Streptococcus sanguis, and Streptococcus salivarius, which contribute to tooth decay and plaque formation. Our current study investigated the efficacy of a colloidal silver gel in inhibiting biofilm formation by these principal oral bacteria, in vitro. The aim of this study was to assess the efficacy of a colloidal silver gel formulation for inhibiting bacterial biofilm formation (Ag-gel) by the principal bacteria that cause plaque formation and tooth decay. Methods: The effect of Ag-gel on viability of S. mutans, S. sanguis, and S. salivarius was assessed by quantifying their colony forming units (CFU) in presence or absence of the test gel. The effect of this formulation on biofilm-forming ability of these bacteria was studied through scanning electron microscopy. Results: Using the CFU assays, over 6 logs of inhibition (100%) were found for S. mutans, S. sanguis, and S....