Application of Response Surface Methodology for Optimization of Culture Medium in Begonia malabarica for In vitro Regeneration (original) (raw)

Abstract

The aim of the present study was to optimize the culture medium components in the growth media of Begonia malabarica in order to enhance the shoot multiplications for extends its applicability to the commercial production of secondary metabolites. Optimization of medium components such as sucrose, 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) was performed by Central Composite Design- Response Surface Methodology (CCD-RSM) to induce shoot multiplication. The maximum shoot multiplication were found to be Murashige and Skoog (MS) basal medium supplemented with 30 g/l of sucrose, 0.5 mg/l of BAP and 0.55 mg/l of NAA with 8.9 number of bud per explant. It was observed that the model predicted values of almost all points were nearer to experimental values. The fully grown explants were transferred to rooting medium at the concentration of 0.5 mg/l NAA. The rooted plantlets were acclimatized with sand, garden soil and farmyard manure at the ratio of 1:1:1 in environmental growth chamber. The present work reveals shoot multiplication efficacy on MS medium at optimized concentrations of growth regulators without expending more media constituents.

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