Validation of diagnostic accuracy using digital slides in routine histopathology (original) (raw)
Related papers
Validation of digital pathology imaging for primary histopathological diagnosis
Histopathology, 2015
Digital Pathology (DP) offers advantages over glass slide microscopy (GS), but data demonstrating a statistically valid equivalent (i.e. non-inferior) performance of DP against GS is required to permit its use in diagnosis. Seventeen pathologists re-reported 3,017 cases by DP. Of these 1,009 were re-reported by the same pathologist and 2,008 by a different pathologist. Re-examination of 10,138 scanned slides (2.22 terabytes) produced 72 variances between GS and DP reports, including 21 clinically significant variances. Ground truth lay with GS in 12 and DP in 9 cases. These results are within the 95% confidence interval for existing intra- and inter- observer variability, proving DP is non-inferior to GS. In three cases the digital platform was deemed responsible for the variance, including a gastric biopsy where Helicobacter pylori only became visible on slides scanned at the x60 setting, and a bronchial biopsy and penile biopsy where dysplasia was reported on DP but not present on...
Validation of Whole-Slide Imaging for Histolopathogical Diagnosis: Current State
Pathobiology, 2016
Rapid advances in informatics and technological improvements have led to the development of high-throughput whole-slide imaging (WSI) scanners able to produce high-quality digital images, which allow achieving a correct diagnosis of the biopsies using virtual viewers. This technology is currently prepared to be introduced in the departments of pathology for routine diagnosis. The aim of this review is to analyze the current evidence regarding the use of WSI in primary or routine diagnosis in the different subspecialties of pathology. An increasing number of studies have shown almost perfect inter- and intraobserver agreement between the diagnoses obtained with WSI and the classical diagnoses based on conventional light microscopy. The only exception seems to be cytology, which still requires some technological development. Although validation studies are needed in some areas of pathology, growing evidence indicates that WSI is a reliable tool for routine diagnosis. Pathologists have...
Diagnostic Pathology, 2016
Introduction/ Background UNIM Ltd. have created the SAAS platform DPathology that can be used for saving and studying histological slides and it doesn’t require an installation of a special software. You can use the platform with all the modern internet browsers. The SAAS platform gives all the specialists a chance to analyze remotely digital histological slides. It increases the accuracy of diagnostics and speeds up the medical assessment Aims To indicate the importance of collecting rare cases and expert assessment via digital microscopy Using the Digital Pathology© platform to carry out educational and competitive diagnostic measures. Methods Fourteen rare cases from different sub-specializations field in pathology were selected by UNIM LTD with expert’s pathologists from the Czech Republic and Italy and additionally validated in Norwayand theUK (blind method). The slides were digitized and introduced withclinical information to 250 specialists registered to take part in the comp...
The advent of digital pathology : A depth review
Journal of Entomology and Zoology Studies, 2019
Digital pathology is an image based information environment which is enabled by computer technology that allows for the management of information generated from a digital slide. Digital pathology is enabled in part by virtual microscopy, which is the practice of converting glass slides into digital slides that can be viewed, managed, shared and analyzed on a computer monitor. Growing demand for accurate and reliable diagnosis along with issues of patient safety is pushing traditional diagnosis towards an update. Over the last two-three decades the field of optics has made great advancements in the form of ever-improving optics and digital cameras. Persistent gains in computer processing power, data transfer speeds, advances in software and cloud storage solutions have enabled the use of digital images for a wide variety of purposes in pathology. High-resolution images are generated from whole glass slides which can be analyzed and managed using software. Digital Pathology has become a useful and valuable tool in clinical and research pathology. A fully digital workflow would mean that image analysis could be performed on any pathology image without the need for specific image preparation. Image analysis software is already widely available, and has FDA regulatory approval. The digital decade will likely redefine how pathology is practiced and the role of the pathologist.
Veterinary Pathology, 2019
Digital slides created by whole-slide imaging scanners can be evaluated by pathologists located in remote sites, but the process must be validated before this technology can be applied to routine cytological diagnosis. The aim of this study was to validate a whole-slide imaging scanner for cytological samples. Sixty cytological samples, whose diagnoses were confirmed by gold-standard examinations (histology or flow cytometry), were digitalized using a whole-slide imaging scanner. Digital slides and glass slides were examined by 3 observers with different levels of cytopathological expertise. No significant differences were noted between digital and glass slides in regard to the number of cases correctly diagnosed, or the sensitivity, specificity, or diagnostic accuracy, irrespective of the observers’ expertise. The agreements between the digital slides and the gold-standard examinations were moderate to substantial, while the agreements between the glass slides and the gold-standard...