AFLP and SRAP markers linked to the mj gene for root-knot nematode resistance in cucumber (original) (raw)
Root-knot nematode (RKN) disease infection dynamics is a serious problem in vegetable and fruit production (Qi et al. 2018) and significantly hampers the yield. The most important pathogens present in the root zone area of plants are nematodes that minimise the plant growth and production. Meloidogyne is the most common genus among the nematodes of greenhouse vegetable crops (Tileubayeva et al. 2021). Nematodes are devastating pests that infect most cultivated plant species causing considerable agricultural losses throughout the world (Eder et al. 2018). Meloidogyne incognita attacks the most economically important fruits and vegetable crops, including Citrullus lanatus (Bello et al. 2021), Solanum lycopersicum (Eder et al. 2021) and Cucumis sativus (Kayani et al. 2017) and causes enormous losses around the world (Jones et al. 2013). The intense economic importance of rootknot nematodes (Meloidogyne spp.) in agriculture has elicited the interest of a large number of researchers and institutions to study them. The total yield losses due to this nematode are about
Journal of Heredity, 2014
Root-knot nematodes limit carrot production around the world by inducing taproot forking and galling deformities that render carrots unmarketable. In warmer climates, Meloidogyne javanica and Meloidogyne incognita are most prevalent. In F 2 and F 3 progeny from the cross between an Asian carrot resistant to M. javanica, PI 652188, and a susceptible carrot, resistance response was incompletely dominant with a relatively high heritability (H 2 = 0.78) and provided evidence for a single gene, designated Mj-2, contributing to resistance. Molecular markers linked to the previously described root-knot nematode resistance gene, Mj-1 on chromosome 8 derived from "Brasilia," demonstrated that Mj-2 does not map to that same locus but is on the same chromosome.
Journal of Heredity, 2013
Root-knot nematodes limit carrot production around the world by inducing taproot forking and galling deformities that render carrots unmarketable. In warmer climates, Meloidogyne javanica and Meloidogyne incognita are most prevalent. In F 2 and F 3 progeny from the cross between an Asian carrot resistant to M. javanica, PI 652188, and a susceptible carrot, resistance response was incompletely dominant with a relatively high heritability (H 2 = 0.78) and provided evidence for a single gene, designated Mj-2, contributing to resistance. Molecular markers linked to the previously described root-knot nematode resistance gene, Mj-1 on chromosome 8 derived from "Brasilia," demonstrated that Mj-2 does not map to that same locus but is on the same chromosome.
TAG Theoretical and Applied Genetics, 2000
Inheritance studies have indicated that resistance to the root-knot nematode (Meloidogyne javanica) in carrot inbred line 'Brasilia-1252' is controlled by the action of one or two (duplicated) dominant gene(s) located at a single genomic region (designated the Mj-1 locus). A systematic search for randomly amplified polymorphic DNA (RAPD) markers linked to Mj-1 was carried out using bulked segregant analysis (BSA). Altogether 1000 ten-mer primers were screened with 69.1% displaying scorable amplicons. A total of approximately 2400 RAPD bands were examined. Four reproducible markers (OP-C2 1700 , OP-Q6 500 , OP-U12 700 , and OP-AL15 500) were identified, in coupling-phase linkage, flanking the Mj-1 region. The genetic distances between RAPD markers and the Mj-1 locus, estimated using an F 2 progeny of 412 individuals from 'Brasilia 1252' × 'B6274', ranged from 0.8 to 5.7 cM. The two closest flanking markers (OP-Q6 500 and OP-AL15 500) encompassed a region of 2.7 cM. The frequency of these RAPD loci was evaluated in 121 accessions of a broadbased carrot germplasm collection. Only five entries (all resistant to M. javanica and genetically related to 'Brasilia 1252') exhibited the simultaneous presence of all four markers. An advanced line derived from the same cross, susceptible to M. javanica but relatively resistant to another root-knot nematode species (M. incognita), did not share three of the closest markers. These results suggest that at least some genes controlling resistance to M. incognita and M. javanica in 'Brasilia 1252' reside at distinct loci. The low number of markers suggests a reduced amount of genetic divergence between the parental lines at the region surrounding the target locus. Nevertheless, the low rate of recombination indicated these markers could be useful landmarks for positional cloning of the resistance gene(s). These RAPD markers could also be used to increase the Mj-1 frequency during recurrent selection cycles and in backcrossing programs to minimize 'linkage drag' in elite lines employed for the development of resistant F 1 hybrids.
Root-knot Nematode Resistance in Cucumber and Horned Cucumber
HortScience, 1993
Cucumber (Cucumis sativus L.) and horned cucumber (C. metuliferus Naud.) germplasm were evaluated for their resistance to root-knot nematodes (Meloidogyne spp.). All 24 C. metuliferus cultigens evaluated were resistant to all root-knot nematodes tested-M. incognita (Kofoid and White) Chitwood race 3, M. arenaria (Neal) Chitwood race 2, and M. hapla Chitwood. All 884 C. sativus cultigens (cultivars, breeding lines, and plant introduction accessions) tested were resistant to M. hapla and few to M. incognita race 3. Only 50 of 884 C. sativus cultigens evaluated were somewhat resistant to M. arenaria race 2 and M. incognita race 3. A retest of the most resistant C. sativus cultigens revealed that LJ 90430 [an accession of C. sativus var. hardwickii (R.) Alef.] and `Mincu' were the only cultigens that were moderately resistant to M. arenaria race 2. LJ 90430 was the only cultigen, besides the two retested C. metuliferus cultigens, that was resistant to M. javanica (Treub) Chitwood. A...
Chilean journal of agricultural research, 2022
The root-knot nematode Meloidogyne spp. causes yield losses of up to 68% on tomato (Solanum lycopersicum L.) crops. Genetic resistance in the host plant makes the crop sustainable and it can breakdown when there is a high population density of the pathogen. The objective of this study was to determine the nematode population density that allow determining the resistance potential of tomato germplasm associated with the Mi-1 resistance gen. The Mi-1 gene was evaluated with the molecular marker SCAR Mi-23 and specific primers in the genotypes COLY007, IAC1687, LA0445, IAC1622 and two commercial controls (susceptible and resistant). The damage scale and the number of individuals recovered (eggs and juveniles) were assessed, with different population densities of the pathogen inoculated (0, 1000, 2000, 3000, 4000 and 5000 individuals plant-1), in a split plot design, with six replicates and a plant as the experimental unit. The genotype IAC1687 and the resistant commercial control presented the resistance allele of the Mi-1 gene and were classified as moderately resistant to a density of 1000 individuals plant-1. Despite having the Mi-1 gene, the COLY007 genotype was classified as moderately susceptible and with densities greater than 1000 individuals plant-1 can break resistance in all genotypes evaluated. Additionally, it is necessary to correlate the genotypic and phenotypic responses to guarantee the success of the selection supported by molecular markers such as SCAR Mi-23 and identify promising genotypes that could be included in a long-term breeding and also used as rootstocks in an integrated management of root-knot nematode.
Screening Cultigens of Cucumber and Horned Cucumber for Resistance to Root Knot Nematodes
HortScience, 1990
Root knot, caused by Meloidogyne spp. is the most important disease of cucumber (Cucumis sativus L.) in North Carolina, causing an average annual yield loss of 12 %. A greenhouse study was conducted 10 screen 924 cultigens (728 accessions, 136 cultivars and 36 breeding lines of C. sativus, and 24 accessions of C. metuliferus Naud.] for resistance to 3 species of root knot nematodes, M. incognita r. 3, M. arenaria r. 2 and M. hapla, Plants were grown from seed in 150-mm diameter clay pots. Two-week-old seedlings were inoculated with 5000 nematode eggs per plant, then evaluated for resistance 9 weeks later. All cultigens evaluated were resistant to M. hapla. Little resistance was found in the cultigens of C. sativus to M. incognita r. 3 and M. arenaria r. 2. Most of the cultigens evaluated were susceptible to both. `Southern Pickler' was resistant to both nematodes (1 % average galls). `Green Thumb and LJ 90430 were resistant to M. arenaria r. 2, Two check cultigens, `Sumter' ...
The Plant Journal, 1992
A dominant allele at the Milocus on chromosome 6 of tomato (Lycopersicon esculentum Mill) confers resistance to three species of root-knot nematodes (Meloidogyne). The resistance, which is associated with a localized necrotic response, was originally introduced into tomato from the wild species Lycopersiconperuvianum. As a step towards the molecular cloning of Mi, we have identified closely linked DNA markers from both cDNA and genomic DNA libraries as restriction fragment length polymorphisms (RFLPs). DNA from tomato populations segregating for nematode resistance was analyzed to generate a highresolution genetic map of this region. Additional information on gene order was obtained by comparing the size of the introgressed L pemvianum chromosomal segment within a collection of nematode-resistant tomato lines. Among the four cDNA markers that are tightly linked to Mi, three are dominant, i.e. L peruvianumspecific. One cDNA marker corresponds to a gene family comprising 20-30 members, one of which is diagnostic for all nematode-resistant genotypes tested. The presence of non-homologous sequences around the Migene may contribute to the suppression of recombination in this region of the genome in crosses heterozygousfor Mi. The potential of 'walking' from closely linked markers to Mi is discussed.