The combined effect of IDA and glutaraldehyde on the properties of human erythrocytes (original) (raw)
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The effect of selected membrane active substances on erythrocyte deformability
Bratislavske lekarske listy
Aminoguanidine improved the erythrocyte filterability by 4%, pyridoxyliden-aminoguanidine by 11% and pyridoxal by 13% in healthy subjects. In diabetic patients the aminoguanidine effect on erythrocyte filterability was improved by 7%, PAG effect by 9% and pyridoxal effect by 15% in comparison to the control group. The other investigated haematological variables in both groups were within the range of the physiological standard. All of the tested substances demonstrated a mild protective influence on erythrocyte elasticity both in healthy subjects and diabetic patients. Significant elasticity improvement was obtained only by pyridoxal (p<0.01) in patients with diabetes mellitus. (Fig. 4, Ref. 18.).
The effects of glutaraldehyde and osmium tetroxide on the erythrocyte membrane
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1982
A nitroxide spin label probe technique was applied to study the interaction between glutaraldehyde or osmium tetroxide (OsO4) and the membranes of horse erythrocytes, ghosts and liposomes prepared from erythrocyte lipids. Two major conclusions have been established: (1) Reaction of the fixation reagents with the membrane is selective. OsO 4 reacts predominantly with lipids and glutaraldehyde with membrane proteins. (2) The lipid-protein interactions change after pretreatment by OsO 4 or glutaraldehyde.
Charge-independent effects of drugs on erythrocyte morphology
Biochemical Pharmacology, 1986
The effects of ~hlorp~m~ne, tetracaine, i~domethacin, barbitone and benzyl alcohol on human erytbrocyte shape have been examined. Cationic and anionic drugs produced stomatocy~es and echinocytes respectively as expected for cells in isotonic saline. Particular attention has been directed here to some features of drug induced morphology change which are independent of the charge of the drug. It was found that (i) the direction (increase or decrease) of the extent of morphological change as temperature was increased from 20 to 37", (ii) the exposure time for maximum shape change (O-2 min), and (iii) the time course of cell morphology (O-30 min) were different for similarly charged drugs. The influence of low concentrations of the drugs on the thermal fragmentation patterns of the cells has been determined. A single index has been derived which allows comparison of the morphological
Biochemical Pharmacology, 1985
The influence of the anionic drugs indomethacin, barbitone, salicylate and the cationic drugs chlorpromazine and tetracaine on the morphology of human erythrocytes suspended in solutions of different chloride concentration (thus altering cell membrane potential) and constant osmolality, has been examined. As expected, the anionic and cationic drugs produced echinocytes and stomatocytes respectively in 145 mM NaCl. The cationic drugs induced fewer stomatocytes in 60 mM chloride than in 145 mM chloride at 37". Tetracaine induced echinocytes in 60 mM chloride at 20". Indomethacin and barbitone produced echinocytes in 145 mM chloride and stomatocytes in 60 mM chloride. Salicylate no longer produced echinocytes when the chloride concentration was reduced. Cells exposed to salicylate in 60mM chloride were less cupped than the control cells. We suggest that the distribution of the charged form of the drug across the membrane is in equilibrium with the distribution of chloride ions. Changes in the intracellular drug concentration when the extracellular chloride is varied could then account for the observed shape changes in a manner which is consistent with the bilayer couple hypothesis for drug-membrane interactions.
Thrombosis Research, 1985
Electron spin resonance spectroscopy of probed samples was used to determine the structural changes in human erythrocyte membranes prior to and at intervals following ingestion of either 10 grains acetylsalicylic acid, 10 grains sodium salicylate, or 50 mg indomethacin by both male and female subjects. Analysis of erythrocytes from female subjects indicated a time-dependent disordering of the membrane over the eight hour period following aspirin ingestion while the cells of male subjects showed a slight membrane ordering over the same time period. Erythrocytes drawn from females at the beginning of the menstrual cycle showed the greatest amount of membrane disordering at one hour following aspirin ingestion, but by eight hours, the membrane structure had returned to that of control. The time dependent disordering in membrane structure of cells from females in the middle of the menstrual cycle was biphasic. Ingestion of indomethacin induced only slight membrane changes in both male and female subjects over the times examined. Ingestion of sodium salicylate by either men or women did not induce significant changes in erythrocyte membrane order. Washed erythrocytes when mixed with salicylate, aspirin, or indomethacin were either identical to control cells or slightly more ordered. This study suggests that aspirin-induced alterations in membrane structure may depend upon steroid hormone levels.
American Journal of Hematology, 1981
Membrane structure in intact human erythrocytes was analyzed by electronspin-resonance (ESR) spectroscopy. The spin probes 5-doxy1 stearate and 5-doxy1 stearate methyl ester revealed thermally-induced structural transitions in the membrane at 37OC and 15OC. The addition of propranolol, diazepam, chlorpromazine, or Pluronic F68 all caused a decrease in the temperature of the upper transition, but did not markedly alter the temperature of the lower transition. In addition, diazepam caused a significant decrease in the ordering or packing of the membrane-lipid acyl chains. It is proposed here that the protection from hypotonic hemolysis that has been reported in the presence of these drugs is mediated by a structural rearrangement in the erythrocyte membrane involving a change in protein-lipid interactions.
Canadian Journal of Physiology and Pharmacology, 2010
The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p < 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p < 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice...
The Open Biology Journal, 2012
The practical importance for the pharmaceutical and cosmetics industries of the interactions between biological membranes and surfactant molecules has led to intensive research within this area. The interactions of non-ionic surfactant n-octyl-β-D-glucopyranoside (OG) with the human and rat erythrocyte membranes were studied. The in vitro hemolytic and antihemolytic activities were determined by employing a method in which both erythrocytes were added to the hypotonic medium containing OG at different concentrations, and the amount of haemoglobin released was determined. noctyl-β-D-glucopyranoside was found to have a biphasic effect on both types of erythrocyte membrane. We also investigated the interactions of OG with the erythrocyte membrane in isotonic medium; the dose-dependent curves show similar behaviour in both human and rat erythrocytes. Our results showed that OG has greater antihemolytic potency on rat than on human erythrocytes; furthermore, rat erythrocytes were more sensitive than human erythrocytes to hypotonic shock. How the different lipoprotein structure of these erythrocytes determines a difference in antihemolytic activity is discussed.