An Ultra Performance Liquid Chromatography for ISMN (original) (raw)

A highly sensitive, accurate and rapid analytical method based on reverse-phase liquid chromatography/ electrospray ionization tanden mass spectroscopy (RP-LC-ESI-MS/MS) has been developed and validated for the determination of the Isosorbide 5-Mononitrate in human plasma using Isosorbide 13 C6 5-Mononitrate as Internal Standard. The method was validated over a linear range of 7.506 -1926.642 ng/ml, R2 0.9989. After addition of IS, analytes were extracted from the plasma samples by solid phase extraction using HLB cartridge. Chromatographic separations were achieved reversed phase column (Thermo Hypurity C8, 100x4.6 mm, 5 µm particle size) using a mobile phase consisting 1 mM Ammonium acetate (Acidified) and acetonitrile (25: 75, v/v (%)), flow rate 0.60 (mL/min.). Detection utilized a tandem MS/MS, the analytes were ionized using an ESI source in the positive ion mode prior to detection by Multiple Reaction Monitoring (MRM) mode. The analytes were monitored at the following transitions (m/z) 249.90 → 59.00 for Isosorbide 5-Mononitrate and (m/z) 255.90 → 58.90 for Isosorbide 13 C6 5-Mononitrate respectively. The proposed method was fully validated in terms of linearity, accuracy, precision, specificity, sensitivity, recovery and stability, giving results within the acceptable range.

Sign up for access to the world's latest research.

checkGet notified about relevant papers

checkSave papers to use in your research

checkJoin the discussion with peers

checkTrack your impact