Enhanced Biotransformation Capacity of Rhodiola rosea Callus Cultures for Glycosid Production (original) (raw)
Related papers
Plant Science, 2004
Compact callus aggregates (CCA) of Rhodiola rosea were established. The growth curve of CCA culture was determined. No secondary compounds are produced in callus. Cinnamyl alcohol was added to the media in several concentrations between 0.05 and 5 mM in order to stimulate the production of cinnamyl glycosides. The optimal concentration of the precursor was determined. The consumption of cinnamyl alcohol and the production of cinnamyl glycosides were followed by daily sampling. The chemical analyses of the samples were performed by HPLC/MS. Among the cinnamyl glycosides rosin was produced in high amounts. A three to sixfold rosin content was achieved in the treated calli comparing to the content of wild growing plants. Rosavin was produced in traces only.
Rosavin as a product of glycosylation by Rhodiola rosea (roseroot) cell cultures
1999
The paper discusses glycosylation of trans-cinnamyl alcohol to obtain the biologically active compound rosavin and possibly other cinnamoylglycosides. Cell suspension cultures of Rhodiola rosea were established from callus of leaf origin cultured under light in a modified Murashige and Skoog medium. Under these conditions, no rosavin was formed. However, when trans-cinnamyl alcohol (2.5 mM; in MeOH) was added to the medium, after 72 h incubation cells transformed over 90% of the cinnamyl alcohol into a number of unidentified products. The structure of potential rosavin accumulated in intracellular spaces was elucidated as [3-phenyl-2-propenyl-O-(6'-Oα-L-arabinopyranosyl)-β-D-glucopyranoside] by means of chemical and spectral analysis using TLC, HPLC, UV, LSIMS and NMR methods. Rosavin yields of 0.03-1.01% dry weight were obtained. The actual amount depended on the cell strain cultured and the biotransformation period.
Study of Biotransformation Compounds in Callusar Culture of Rhodiola Rosea Specie
2015
Rhodiola rosea L. is a well known species of plants, which has been used medicinally for decades, but study of its pharmacological effects and the compounds responsible for it use still continues. We present data about induced accumulation of secondary metabolites and as well the results of biotransformation of cinamic alcohol in callus culture of R. rosea of Carpathian origin under the influence of same stress factors. The presence of secondary metabolites was investigated by HPLC-MS analysis. The obtained results can be used for selection of valuable genotypes and their future cultivation in artificial conditions.
2008
s:135. 29. Krajewska-Patan A, Furmanowa M, Dreger M, Górska-Paukszta M, Łowicka A, Mścisz A, Mielcarek s, Baraniak M, Buchwald W, Mrozikiewicz PM. enhancing the biosynthesis of salidroside by biotransformation of p-tyrosol in callus culture of Rhodiola rosea L. herba Pol 2007; 53:55-64. 30. Krajewska-Patan A, Dreger M, Łowicka A, Górska-Paukszta M, Mścisz A, Mielcarek s, Baraniak M, Buchwald W, Furmanowa M, Mrozikiewicz PM. chemical investigations of biotransformed Rhodiola rosea callus tissue. herba Pol 2007; 53: 77-87. 31. György z, tolonen A, Pakonen M, neubauer P, hohtola A. enhancing the production of cinamyl glucosides in compact callus aggregate cultures of Rhodiola rosea by biotransformation of cinnamyl alcohol. Plant sci 2004, 166:229-36. 32. György z, tolonen A, neubauer P, hohtola A. enhanced biotransformation capacity of Rhodiola rosea callus cultures for glycoside production. Plant cell tiss organ cult 2005; 83:129-35. 33. Mikołajczak P, okulicz-Kozaryn i, Kamińska e, n...
Proceedings of the Bulgarian Academy of Sciences
Rhodiola rosea L. is a high-altitude medicinal plant with various health-beneficial effects, associated mainly with the phenolic substances salidroside and rosavins (rosavin, rosin and rosarin). The intensive collection of R. rosea rhizomes and roots to be used in folk medicine and pharmacy has led to a decrease of the species natural resources. New opportunities for species conservation and accelerated synthesis of biologically active substances have been provided by various systems for in vitro and ex vitro cultivation. In this study, the salidroside and rosavins content was determined using HPLC analysis in in vitro cultures of R. rosea and further monitored in adapted regenerants grown ex vitro. The targeted secondary metabolites were not detected in the calli and in the roots of 1-month-old regenerants obtained on different nutrient media. The roots/rhizomes of adapted regenerants grown ex vitro in a greenhouse for 1 year and in the mountain for 1, 2 and 3 years produced increa...
Acta Biologica Cracoviensia Series Botanica, 2000
The study examined tyrosol glucosyltransferase activity and the efficiency of salidroside production in natural and transformed root cultures of Rhodiola kirilowii (Regel) Regel et Maximowicz. Neither enzyme activity nor salidroside accumulation were detected in natural and transformed root cultures maintained in media without tyrosol. To induce TGase activity in biotransformation reactions, tyrosol was added to natural and transformed root cultures on the day of inoculation. The first peak of TGase activity (0.23 U/μg) was detected on day 9 in natural root culture, accompanied by the highest salidroside content (15.79 mg/g d.w.), but TGase activity was highest (0.27 U/μg) on day 15. In transformed root culture, day 18 showed the highest TGase activity (0.15 U/μg), which coincided with the highest salidroside content (2.4 mg/g d.w.). Based on these results, tyrosol was added to the medium on the days of highest previously detected activity of TGase: day 15 for natural root cultures and day 18 for transformed root cultures. This strategy gave significantly higher yields of salidroside than in the cultures supplemented with tyrosol on the day of inoculation. In natural root culture, salidroside production reached 21.89 mg/g d.w., while precursor feeding in transformed root cultures caused a significant increase in salidroside accumulation to 7.55 mg/g d.w. In all treatments, salidroside production was lower in transformed than in natural root cultures.
Rhodiola rosea L.: from golden root to green cell factories
Phytochemistry Reviews, 2016
Phytochemical composition of Rhodiola rosea Medicinal use and ethnopharmacology of Rhodiola rosea: a focus on the molecular mediators Clinical trials Preservation strategies of Rhodiola rosea Rhodiola rosea in vitro systems as a source of pharmaceutically valuable secondary metabolites. Biosynthetic pathway of salidroside and cinnamyl alcohol glycosides Biotechnological tools for secondary metabolites enhancement Conclusions and future perspectives
The Scientific World Journal, 2012
At present, more than 50 000 plant species are used in phytotherapy and medicine. About 2/3 of them are harvested from nature leading to local extinction of many species or degradation of their habitats. Biotechnological methods offer possibilities not only for faster cloning and conservation of the genotype of the plants but for modification of their gene information, regulation, and expression for production of valuable substances in higher amounts or with better properties.Rhodiola roseais an endangered medicinal species with limited distribution. It has outstanding importance for pharmaceutical industry for prevention and cure of cancer, heart and nervous system diseases, and so forth. Despite the great interest in golden root and the wide investigations in the area of phytochemistry, plant biotechnology remained less endeavoured and exploited. The paper presents research on initiation ofin vitrocultures inRhodiola roseaand some otherRhodiola species. Achievements in induction o...