Expression of a green fluorescence protein-carrier protein into mouse spermatozoa (original) (raw)
2002, Biochemical and Biophysical Research Communications
Intra-testicular inoculation of an adenoviral vector carrying the fusion gene Aequorea victoria green fluorescence protein/rat-liver glycogen synthase (GFP/LGS) resulted in the presence of GFP/GLS in spermatozoa from 7 days to, at least, 16 days after inoculation. The GFP/LGS was detected in the sperm heads after an ''in vitro'' fertilization procedure, either before or after the oocyte penetration. Our results indicate that spermatozoa carrying GFP/LGS protein conserved their fertilizing ability and were also detectable after oocyte penetration. This technique will allow to develop an easy system to follow the fate of mature sperm proteins.
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