The seed nuclear proteome (original) (raw)

Transcriptome- and proteome-wide analyses of seed germination

Transcriptome- and proteome-wide analyses of seed germination, 2008

Using post-genomic technologies, it is now possible to understand the molecular basis of complex developmental processes. Inthe case of seed germination, recent transcriptome- and proteome-wide studies led to new insights concerning the building up of the germination potential during seed maturation on the mother plant, the reversible character of the first phases of the germinationprocess enabling the imbibed embryo to recapitulate the late maturation program for mounting defense response when confrontedto environmental fluctuations, the timing of expression of genes playing a role in controlling radicle emergence, the role of planthormones as abscisic acid and gibberellins in seed germination, and finally the global changes in proteome activity induced byredox regulation occurring in seed development and germination. In this way, post-genomic technologies help facilitating the advent of a systems approach to uncover novel features of seed quality, which can lead to potential applications, for example inselection programs.

Proteome of seed development and germination (Book chapter)

Chapter 13. Proteome of Seed Development and Germination, 2008

Seeds are the main propagation unit for plant growth and they are also preponderant for food supply due to their accumulated compounds. Proteomics have been instrumental to characterize mechanisms involved in the transition from the developing to the germinating seeds. These studies provided a comprehensive picture of metabolic control and switches during development, dormancy, germination and seedling establishment. A dynamic proteomic approach, based on the characterization of the de novo synthesized proteome, revealed many additional features compared to classical proteomics.

Discovering Seed Proteome: Challenges and Perspectives

For centuries, crop plants have represented the basis of the daily human diet. Among them, cereals and legumes, accumulating oils, proteins and carbohydrates in their seeds, distinctly dominate modern agronomic practice. Indeed, these plants play an essential role in the food industry and fuel production. Therefore, the seeds of crop plants are intensively studied by food chemists, biologists, biochemists, and nutritional physiologists. Accordingly, not only seed development and germination, but also age- and stress-related alterations in seed vigor, longevity, nutritional value and safety can be addressed by a broad panel of analytical, biochemical and physiological methods. Currently, functional genomics is one of the most powerful tools, giving direct access to characteristic metabolic changes, accompanying plant development, senescence and response to biotic or environmental stress. Among individual methodological platforms, proteomics represents one of the most effective ones, ...