Biochemistry-lab-manual-spring 2009 (original) (raw)

Techniques in Molecular Biology Lab Manual 2

Shortage of adequate reference books, teaching materials (modules), lab manuals, chemicals and equipment’s are the bottle necks in biotechnology. It is understood that the availability of well complied teaching materials promote quality education. This manual tries to address and compile some of the routinely used protocols in techniques in molecular biology for easy access. The aim of this manual is not only to bring together the protocols, but also to understand some of the basics behind following the methodologies. This material primarily prepared for the course technique in molecular biology, but have indefinite role in the practical aspects genetic engineering, forensic science, marker, enzyme technology and etc. In view of the fact that, lack of well-organized text (reference) book with full practical aspect (lab manual) as per harmonized curriculum preparing this material for stated course gate great concern. From this teaching material the lecturer and students will gate benefit accordingly. . The target is to give students a view of techniques in molecular biology, especially those who have just ventured into the field of biotechnology and other life sciences. The protocols are made easy to follow with each step explained in layman,’ simple terms. Even though the field of biotechnology is exhaustive, an effort has been made to list some of the protocols that could serve as a foundation for starting any molecular investigation. This material made to enclose both theoretical part and practical aspects which equip the user with various techniques employed in biotechnology. We would like to thank all the members of Biotechnology staff of Wachemo University for their valuable advice. We also present our appreciation to Wachemo University press for unending support

EXPERIMENTAL BIOCHEMISTRY THEORY, EXPERIMENT, ANALYSIS AND REPORTING F 2007 Version

ACKNOWLEDGEMENTS This manual has benefited tremendously from feedback and input provided by students and teaching assistants from the Department of Molecular and Structural Biochemistry at North Carolina State University. We thank them for their patience as the manuscript was being refined and many helpful suggestions. We thank the following specifically for their contributions: Trino Ascencio-Ibanez-who developed the ideas and techniques that were incorporated into the viral Southern blot lab Emily Blake-who helped refine the format and wrote parts of the following sections: the Excel explanation, some Prelab questions, Terms, Methods and Report Writing. Benjamin Bobay-who helped adapt and flesh out the methodology in amino acid pH titration lab Audrey Goodell-who adapted methodology to develop the affinity chromatography technology in the LDH lab Jesse Gore-who wrote the pH meter section and assisted with global editing Daniel Kahl-who developed portions of the PCR and viral South...

An undergraduate biochemistry laboratory course with an emphasis on a research experience

Biochemistry and Molecular Biology Education, 2003

In their junior or senior year, biochemistry majors at the University of Detroit Mercy are required to take a two-credit biochemistry laboratory course. Five years ago, the format of this course was changed from structured experiments to a more project-based approach. Several structured experiments were included at the beginning of the course because not all students were familiar with computer-based statistics and graphics programs or fundamental biochemical techniques. Finally, students were given an enzyme purification assignment and a test/control project. In the latter project, students were provided with tissue from test and control animals and were expected to propose and test a parameter that they hypothesized might be different between the two tissues. For both projects, student teams were required to search the literature, submit and orally defend their proposals, perform the experiments, and submit a report. For all experiments except the test/control project, students submitted a written report in the style of a journal paper. A poster presentation was required for the test/control project. In course evaluations, the students cited a high degree of satisfaction with the project-based approach.