Modulation of dab (Limanda limanda, L.) macrophage respiratory burst activity (original) (raw)

Fish & Shellfish Immunology, 1996

Abstract

ABSTRACT DabLimanda limandakidney macrophages were isolated by adherance to tissue culture plates and their respiratory burst activity subsequently analysed. Several seeding cell concentrations were tested, with 107and 2×107cells ml−1giving significant respiratory burst activity. Respiratory burst activity of the isolated macrophages was also investigated following a preincubation for 24h or 48h with a variety of concentrations of human recombinant tumor necrosis factor α(rTNFα),β-glucan, Concanavalin-A (Con-A) or lipopolysaccharide (LPS). All four stimulants were able to up-regulate dab macrophage respiratory burst activity at particular concentrations, especially after a 48h incubation when 6·25 and 12·5iuml−1rTNFα, 0·125, 0·25 and 0·5μgml−1β-glucan, 1·25 and 2·5μgml−1Con-A and 3·125, 6·25, 12·5, 25 and 50μgml−1LPS induced significant increases. LPS and Con-A gave the largest relative increases in activity. However, the highest concentrations tested (50 and 100iuml−1rTNFα, 2, 4 and 8μgml−1β-glucan, 80μgml−1Con-A and 200μgml−1LPS) were inhibitory. The data are discussed in relation to the mode of action of each compound and the usefulness of such assays in pollution monitoring.

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